Hyaluronan

Manufactured by Lifecore Biomedical

Sourced in United States

Hyaluronan is a naturally-occurring polysaccharide that is a key component of the extracellular matrix in various tissues. It plays a crucial role in maintaining the structural and functional integrity of these tissues. Lifecore Biomedical's hyaluronan product is a high-quality, pharmaceutical-grade material that can be used in a variety of laboratory applications.

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Lab products found in correlation

Epigallocatechin gallate (egcg), by Merck Group (2 mentions) Gelatin type a, by Merck Group (2 mentions) Hrp conjugated secondary antibody, by Cytiva (1 mentions) Anti cd44, by R&D Systems (1 mentions) 3h cholesteryl hexadecyl ether 3h che, by PerkinElmer (1 mentions) Dapi 4 6 diamidino 2 phenylindole, by Thermo Fisher Scientific (1 mentions) Solvable, by PerkinElmer (1 mentions) 1 2 dipalmitoyl sn glycero 3 phosphocholine, by Avanti Polar Lipids (1 mentions) Doxorubicin, by LC Laboratories (1 mentions) Cholesterol, by Avanti Polar Lipids (1 mentions) Fucoidan, by Merck Group (1 mentions) Fucus vesiculosus, by Merck Group (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions) Phosphate buffered saline (pbs), by Merck Group (1 mentions) 5 5 dimethyl 1 pyrroline n oxide dmpo, by Merck Group (1 mentions) Ascorbic acid, by Merck Group (1 mentions) Poly l lysine coated glass coverslips, by BD (1 mentions) Cy7 n hydroxysuccinimide nhs ester, by Lumiprobe (1 mentions) Lysotracker blue, by Thermo Fisher Scientific (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)

8 protocols using hyaluronan

CD44-Mediated Hyaluronan Signaling

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Anti-CD44 was obtained from R&D systems, UK. HRP-conjugated secondary antibodies were obtained from Amersham, UK and Hyaluronan (MW220 kDa) from Lifecore Biomedical (MN, USA). Cells were stimulated with 100 μg/ml HA for indicated times. All other reagents were purchased from Sigma (Poole, UK) unless otherwise stated.

McFarlane S., Coulter J.A., Tibbits P., O'Grady A., McFarlane C., Montgomery N., Hill A., McCarthy H.O., Young L.S., Kay E.W., Isacke C.M, & Waugh D.J. (2015). CD44 increases the efficiency of distant metastasis of breast cancer. Oncotarget, 6(13), 11465-11476.

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Lipid-Based Nanoparticle Preparation

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1,2-Dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), 1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine (DPPE), and cholesterol were purchased from Avanti Polar Lipids (Alabaster, AL, USA). ³H-Cholesteryl hexadecyl ether (³H-CHE) was purchased from PerkinElmer (Boston, MA, USA). DPPE-mPEG5000 sodium salt was a kind gift from Lipoid GmbH (Newark, NJ, USA). Hyaluronan of various MWs (5–8, 50–60, and 175–350 kDa) was purchased from Lifecore Biomedical (Chaska, MN, USA). Hexadecyltrimethylammonium bromide (CTAB), 1-ethyl-3-(3-(dimethylamino)propyl)carbodiimide (EDC), and N-hydroxysulfosuccinamide (NHSS) were purchased from Sigma-Aldrich (St. Louis, MO, USA). The fluorescent dyes DiD (1,1′-dioctadecyl-3,3,3′,3′-tetramethylindodicarbocyanine, 4-chlorobenzenesulfonate salt) and DAPI (4, 6-diamidino-2-phenylindole) were obtained from Invitrogen (Carlsbad, CA, USA). Doxorubicin was obtained from LC Laboratories (Woburn, MA, USA). Scintisafe 30% cocktail scintillation fluid was purchased from Fisher Scientific (Pittsburgh, PA, USA). Solvable was purchased from PerkinElmer. All other chemicals were purchased from Sigma-Aldrich and were of standard analytic grade or higher.

Qhattal H.S., Hye T., Alali A, & Liu X. (2014). Hyaluronan Polymer Length, Grafting Density, and Surface Poly(ethylene glycol) Coating Influence in Vivo Circulation and Tumor Targeting of Hyaluronan-Grafted Liposomes. ACS Nano, 8(6), 5423-5440.

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Hyaluronan and Fucoidan Bioactive Compounds

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Hyaluronan (molecular weight 200 kDa) was purchased from Lifecore Biomedical, LLC (Chaska, MN, USA). Fucoidan was purchased from ChamBio (Taichung, Taiwan). Fucoidan with high molecular weight (purity ≥ 95%, molecular weights 20~200 kDa) was purified from Fucus vesiculosus (Sigma-Aldrich, St Louis, MO, USA). Type A gelatin (molecular weight 25 kDa), 3-(4,5-dimethyl-thiazol-yl)-2,5-diphenyltetrazolium bromide (MTT), fetal bovine serum (FBS), bovine serum albumin (BSA), phosphate-buffered saline (PBS), FLA, R6G, DAPI, and EGCG were purchased from Sigma-Aldrich (St. Louis, MO, USA). All chemicals and reagents were of analytical grade.

Ho C.H., Chu P.Y., Peng S.L., Huang S.C, & Lin Y.H. (2020). The Development of Hyaluronan/Fucoidan-Based Nanoparticles as Macrophages Targeting an Epigallocatechin-3-Gallate Delivery System. International Journal of Molecular Sciences, 21(17), 6327.

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Antioxidant Hyaluronan Characterization

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Hyaluronan (M_w = 1.69 MDa, M_w/M_n = 1.64) was obtained from Lifecore Biomedical Inc., Chaska, MN, USA (content of transition metals: copper <1 ppm, iron 6 ppm). NaCl p.a. and CuCl₂·2H₂O p.a. were purchased from Slavus Ltd., Bratislava, Slovakia. Ascorbic acid used was obtained from Merck KGaA, Darmstadt, Germany. Aqueous solutions of MnTE-2-PyP⁵⁺, 19.6 mM, MnTnHex-2-PyP⁵⁺, 7.23 mM, and MnTnBuOE-2-PyP⁵⁺, 5.69 mM were obtained from Batinic-Haberle’s lab, Duke University School of Medicine, North Carolina, USA. MnTM-4-PyP⁵⁺ was obtained from the University of Aveiro, Aveiro, Portugal and was prepared via alkylation of metal-free ligand followed by its metalation. 5,5-Dimethyl-1-pyrroline N-oxide (DMPO, ≥97%) was purchased from Sigma-Aldrich Chemie GmbH, Steinheim, Germany. Deionised high-purity grade water, with conductivity of ≤0.055 µS/cm, was made by using the TKA water purification system (Water Purification Systems GmbH, Niederelbert, Germany).

Valachová K., Rapta P., Moura N.M., Batinic-Haberle I, & Šoltés L. (2021). Ortho Isomeric Mn(III) N-Alkyl- and Alkoxyalkylpyridylporphyrins—Enhancers of Hyaluronan Degradation Induced by Ascorbate and Cupric Ions. International Journal of Molecular Sciences, 22(16), 8608.

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Characterization of MDA-1986 Oral Carcinoma Cells

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All chemicals and cell culture supplies were obtained from Fisher Scientific (Pittsburgh, PA) and used as received unless stated otherwise. The internal standard solution and multi-element tuning solution for ICP-MS were purchased from VHG Labs (Manchester, NH). All antibodies were purchased from Ab cam (Cambridge, MA) or Santa Cruz Biotechnology (Santa Cruz, CA) and used as received. Hyaluronan was purchased from Lifecore Biomedical (Chaska, MN). The poly-L-Lysine coated glass coverslips were purchased from BD Biosciences (Franklin Lakes, NJ). The Cy7 N-hydroxysuccinimide (NHS) ester was purchased from Lumiprobe (Hallandale Beach, FL). The Lysotracker blue™ was purchased from Invitrogen. The human oral squamous carcinoma cell line, MDA-1986, was a gift from Dr. Jeffrey Myers (University of Texas, M.D. Anderson Cancer Center; Houston, TX).

Cai S., Alhowyan A.A., Yang Q., Forrest W.C., Shnayder Y, & Forrest M.L. (2014). Cellular Uptake and Internalization of Hyaluronan-based Doxorubicin and Cisplatin Conjugates. Journal of drug targeting, 22(7), 648-657.

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Multifunctional Nanocarrier for Targeted Imaging and Therapy

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Hyaluronan and FU were purchased from Lifecore Biomedical, LLC (Chaska, MN, United States) and Chambio, Co., Ltd. (Taichung, Taiwan). Oleic-acid-coated IO nanoparticles (5 nm in diameter) dispersed in chloroform were obtained from AC Diagnostics, Inc. (Fayetteville, AR, United States). The N-hydroxylsuccinimide (NHS) functionalized methoxy polyethylene glycol (molecular weight 5,000 Da) was obtained from Nanocs, Inc. (New York, NY, United States). The VivoTag^®-S 750 (VT750) is an amine reaction (NHS ester) near-infrared fluorochrome (excitation/emission at 750/775) purchased from PerkinElmer, Inc. (Waltham, MA, United States). Type A gelatin (molecular weight 25,000 Da), dimethyl sulfoxide, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), rhodamine 6G (R6G), fluoresceinamine (FA), fluorescein isothiocyanate (FITC), 4′,6-diamidino-2-phenylindole (DAPI), phosphate-buffered saline (PBS), puromycin, EGCG, and Triton X-100 were purchased from Sigma-Aldrich (St Louis, MO, United States). Fetal bovine serum (FBS), Roswell Park Memorial Institute Medium, penicillin-streptomycin were from Gibco (Brooklyn, New York, United States). All other chemicals and reagents were of analytical grade.

Peng S.L., Lai C.H., Chu P.Y., Hsieh J.T., Tseng Y.C., Chiu S.C, & Lin Y.H. (2020). Nanotheranostics With the Combination of Improved Targeting, Therapeutic Effects, and Molecular Imaging. Frontiers in Bioengineering and Biotechnology, 8, 570490.

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Layilin Regulation of Intestinal Barrier

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Female C57BL/6J mice were obtained from Jackson Laboratories (Bar Harbor, ME). The Lieber-DeCarli high-fat liquid diet (#710260) was from Dyets, Bethlehem, PA. Caco-2 cells were purchased from the American Type Culture Collection (Manassas, VA). Hyaluronan with a molecular weight distribution of 21–40kDa (HA35)(catalog # HA20K-5) was from Life Core Biomedical (Chaska, MN). Primary antibodies were purchased from the following sources: rabbit anti-ZO-1 (Life Technologies, 61–7300), guinea pig anti-occludin (Hycult HP9047), rabbit anti-ß-actin (Cell Signaling, 4967), rabbit anti-Layilin (Bioss, 1842R). Secondary antibodies included donkey anti-rabbit 488 (A21206) and goat anti-guinea pig 568 (A11075) from Molecular Probes, Thermofisher Scientific (Waltham, MA) and goat anti-guinea pig horse radish peroxidase (sc-2438) from Santa Cruz (Dallas, TX). On-target plus smart pool siRNA directed against Layilin was obtained from Dharmacon (Lafayette, CO). Caco-2 cells were transfected using DharmaFECT 2 (Lafayette, CO). Caco-2 cells were cultured in 6.5mm transwell filters (Corning, 3.0 μm pore size) purchased from Thermofisher Scientific (Waltham, MA). Transwell filters for imaging studies were coated with 200 μl of a 0.004% solution of rat-tail collagen (Sigma-Aldrich, St Louis, MO) and transwells used for TEER were coated with type I collagen (Corning, #354236) dissolved in ethanol.

Bellos D.A., Sharma D., McMullen M.R., Wat J., Saikia P., de la Motte C.A, & Nagy L.E. (2019). Specifically-sized hyaluronan (35 kDa) prevents ethanol-induced disruption of epithelial tight junctions through a Layilin-dependent mechanism in Caco-2 cells. Alcoholism, clinical and experimental research, 43(9), 1848-1858.

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Synthetic Compounds for Cancer Research

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Hyaluronan (5000 g/mol) was from Lifecore Biomedical; near infrared dye Dy-700 was from Life Technologies. Vorinostat (SAHA, MK0683), and gefitinib (ZD1839) were obtained from Selleckchem (Munich, Germany). γ-benzyl-L-glutamate Ncarboxyanhydride (NCA-BLG) was purchased from Isochem (France) and use as received.

Jeannot V., Gauche C., Mazzaferro S., Couvet M., Vanwonterghem L., Henry M., Didier C., Vollaire J., Josserand V., Coll J.L., Schatz C., Lecommandoux S, & Hurbin A. (2018). Anti-tumor efficacy of hyaluronan-based nanoparticles for the co-delivery of drugs in lung cancer. Journal of controlled release : official journal of the Controlled Release Society, 275.

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