Total RNA isolation was performed using the ReliaPrep RNA Cell Minprep System (Promega). RNA quality was evaluated using the NanoDrop 2000 spectrophotometer (Thermo Fisher). Total RNA was reverse‐transcribed into cDNA using GoScript Reverse Transcription System. qRT‐PCR was performed using the QuantStudio 7 Flex Real‐Time PCR System (Life Technologies, Waltham, MA, USA), and the GoTaq qPCR Master (Promega, Madison, WI, USA) according to the manufacturer's instructions. Primer sequences used for qRT‐PCR are provided in Supporting Information Table 4.
Reliaprep rna cell minprep system
Manufactured by Promega
The ReliaPrep RNA Cell Miniprep System is a lab equipment product designed for the isolation of total RNA from various cell types. It provides a simple and efficient method for extracting high-quality RNA from small sample sizes.
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Lab products found in correlation
3 protocols using reliaprep rna cell minprep system
1
Gene Expression Analysis by qRT-PCR
2
Gene Expression Profiling of Cell Lines
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The SurePrint G3 Human Gene Expression 8 × 60K v2 Microarray kit from Agilent were used for the gene expression assay of each cell line with two biological replicates. RNA samples were prepared from by ReliaPrep RNA Cell Minprep System (Promega). Data were analyzed using Genespring software.
3
Total RNA Isolation and qRT-PCR Analysis
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Total RNA isolation was performed using the ReliaPrep RNA Cell Minprep System (Promega). RNA quality was evaluated using the NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific), and reverse transcription was performed using the GoScript Reverse Transcription System. Quantitative RT-PCR was performed using the QuantStudio 7 Flex Real-Time PCR System (Thermo Fisher Scientific) and the GoTaq qPCR Master (Promega) according to the manufacturer’s instructions. Primer sequences used for quantitative RT-PCR are provided in Table S1.
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