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Rabbit anti gm130

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Rabbit anti-GM130 is a primary antibody that recognizes the Golgi matrix protein GM130. GM130 is a cis-Golgi protein that plays a role in the structure and function of the Golgi apparatus. This antibody can be used to detect and localize GM130 in various applications, such as Western blotting and immunofluorescence.

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Lab products found in correlation

Chicken anti gfp, by Abcam (4 mentions) Rabbit anti rab5, by Abcam (3 mentions) Mouse anti gm130, by BD (3 mentions) Fluorescent conjugated secondary antibody, by Jackson ImmunoResearch (3 mentions) Mouse anti cytochrome c, by BD (3 mentions) Lsm 710 confocal microscope, by Zeiss (3 mentions) Lsm 780 confocal microscope, by Zeiss (3 mentions) Lsm 900 confocal microscope, by Zeiss (3 mentions) Mouse anti kdel, by Abcam (2 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (2 mentions) Mouse anti golgin 97, by Thermo Fisher Scientific (2 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (2 mentions) Alexa 594, by Thermo Fisher Scientific (2 mentions) Alexa 488, by Thermo Fisher Scientific (2 mentions) Lsm 700, by Zeiss (2 mentions) Ripa buffer, by Thermo Fisher Scientific (2 mentions) Mouse anti ha tag, by Merck Group (1 mentions) Goat anti rabbit dylight 488, by Thermo Fisher Scientific (1 mentions) Anti β cop, by Merck Group (1 mentions) Goat anti mouse immunoglobulin g igg, by Jackson ImmunoResearch (1 mentions)

Close spelling variants of this product

GM130 (58 citations) Anti-GM130 (33 citations) Rabbit anti- (5 citations) Rabbit anti-GM130 antibody (3 citations)

29 protocols using rabbit anti gm130

1

Comprehensive Antibody Characterization Protocol

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Mouse anti-HA tag, anti–β-COP, and anti-tubulin were from Sigma-Aldrich, and anti-GFP was from Santa Cruz Biotechnology (Dallas, TX). Rabbit anti–GM-130 was from Abcam (Cambridge, England). Mouse anti-human ERManI was a kind gift from Richard Sifers (Baylor College, Houston, TX) (used only for human cells) or was from Santa Cruz Biotechnology (used for mouse cells). Rabbit anti-calnexin was from Sigma-Aldrich, and anti-dsRED was from MBL. Goat anti-mouse immunoglobulin G (IgG) linked to horseradish peroxidase (HRP) and goat anti-rabbit IgG-HRP were from Jackson Labs (West Grove, PA). Goat anti-mouse Dylight 594 and goat anti-rabbit Dylight 488 were from Thermo Scientific (Barrington, IL). Rabbit polyclonal anti-H2 N-terminus was previously described (Tolchinsky et al., 1996 (link)), Goat anti-mouse IgG linked to agarose beads was from Sigma-Aldrich. Rabbit anti-Cab45 was described before (Scherer et al., 1996 (link)).
Benyair R., Ogen-Shtern N., Mazkereth N., Shai B., Ehrlich M, & Lederkremer G.Z. (2015). Mammalian ER mannosidase I resides in quality control vesicles, where it encounters its glycoprotein substrates. Molecular Biology of the Cell, 26(2), 172-184.
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2

Immunofluorescence Staining of Viral Infection

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Freshly grown MDBK cell monolayers on glass coverslips were infected with the indicated viruses. At different time points, the cells were fixed in 4% paraformaldehyde, permeabilized with 0.2% Triton X-100, and blocked with 2% bovine serum albumen and 1% goat serum. After washing, the cells were incubated with mouse anti-HA (1:500 dilution) and rabbit anti GM130 (Abcam, Cambridge, UK) (1:250 dilution) antibodies. After subsequent washing, the cells were stained with Cy3-conjugated goat anti-mouse (1:1,000 dilution) and fluorescein isothiocyanate (FITC)-conjugated goat anti-rabbit (1:1,000 dilution) antibodies. After three washes, nuclei were stained with 4, 6-diamidino-2-phenylindole (DAPI). Then, the coverslips were mounted onto slides using anti-fade mounting medium. Images were examined and captured with a Zeiss LSM 510 laser-scanning confocal microscope (Carl Zeiss, Jena, Germany).
Raza S., Deng M., Shahin F., Yang K., Hu C., Chen Y., Chen H, & Guo A. (2016). A bovine herpesvirus 1 pUL51 deletion mutant shows impaired viral growth in vitro and reduced virulence in rabbits. Oncotarget, 7(11), 12235-12253.
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3

Immunofluorescence Labeling of Drosophila Tissues

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The chitin-binding probe was prepared in our laboratory as described [91 (link)]. Chitin-546 was used at 1:10 dilution. The following primary antibodies were used: Chicken anti-GFP (1:200) (Abcam), Guinea Pig anti-Uif (1:300) [69 (link)], Mouse anti-Gasp (1:5) (DSHB), Guinea Pig anti-Cor (1:500) [66 (link)], Rabbit anti-Verm (1:100) [27 (link)], Rabbit anti-Kune-kune (1:200) [43 (link)], Mouse anti-DLG (1:1) (DSHB), Mouse anti-Spectrin (1:10) (DSHB), Mouse anti-Cnx99A (1:5) (DSHB), Rabbit anti-GM130 (1:200) (Abcam), Mouse anti-KDEL (1:200) (Abcam), Rabbit anti-Rab5 (1:200) (Abcam), Rat anti-Rab11 (1:100) [92 (link)], and Mouse anti-Rab7 (1:10) (DSHB). Antibodies generated in this study: Rabbit anti-Osi19 (1:300), Rat anti-Osi15 (1:25), Guinea Pig anti-Osi9 (1:10).
The following secondary antibodies (Invitrogen life technologies) were used: AlexaFluor 488 anti-chicken IgG, anti-rat IgG, anti-mouse IgG, anti-rabbit IgG, anti-guinea pig IgG; AlexaFluor 543 anti-rat IgG, anti-rabbit IgG, anti-guinea pig IgG, anti-mouse IgG; AlexaFluor 594/546 anti-mouse IgM; AlexaFluor 647 anti-rat IgG, anti-mouse IgG, anti-rabbit IgG, anti-guinea pig IgG; AlexaFluor 647 plus anti-mouse, anti-rabbit; AlexaFluor 488 plus anti-mouse, anti-rabbit), and AlexaFluor 405 anti-Guinea Pig. All secondary antibodies were used at 1:200 dilution.
Scholl A., Ndoja I., Dhakal N., Morante D., Ivan A., Newman D., Mossington T., Clemans C., Surapaneni S., Powers M, & Jiang L. (2023). The Osiris family genes function as novel regulators of the tube maturation process in the Drosophila trachea. PLOS Genetics, 19(1), e1010571.
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4

Drosophila Whole Mount and Tissue Section Imaging

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For whole mount staining, we dissected fly tissue samples in ice cold PBS and fixed them with 4% paraformaldehyde at room temperature for 30 min as described [6] (link). For tissue sections, we fixed fly tissues with 4% paraformaldehyde, dehydrated the tissues with acetone, and embedded them in LR white resin (Electron Microscopy Sciences). Images were captured with a Zeiss LSM 710 confocal microscope. Antibodies were used at the following concentrations: rabbit anti-Rab5 (abcam), 1∶500; rabbit anti-Avl [84] (link), 1∶500; rabbit anti-Rab7 [76] (link), 1∶500; mouse anti-PDI (abcam), 1∶100; rabbit anti-GM130 (abcam), 1∶500; rabbit anti-Rab11 (abcam, [6] (link)), 1∶500; chicken anti-GFP (abcam), 1∶1,000; mouse anti-Rh1 [4C5, Developmental Studies Hybridoma Bank (DSHB)], 1∶50; mouse anti-Wash (P3H3, [131] (link)), 1∶20; guinea pig anti-Vps26, 1∶1,000 (this study); biotin-conjugated PNA (Vector Labs), 1∶1,000; Alexa 488-conjugated phalloidin (Invitrogen), 1∶100; and Alexa 405-, Alexa 488-, Cy3-, or Cy5-conjugated secondary antibodies (Jackson ImmunoResearch), 1∶600.
Wang S., Tan K.L., Agosto M.A., Xiong B., Yamamoto S., Sandoval H., Jaiswal M., Bayat V., Zhang K., Charng W.L., David G., Duraine L., Venkatachalam K., Wensel T.G, & Bellen H.J. (2014). The Retromer Complex Is Required for Rhodopsin Recycling and Its Loss Leads to Photoreceptor Degeneration. PLoS Biology, 12(4), e1001847.
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5

Antibody sourcing for cell biology

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Several antibodies were generous gifts: rabbit anti-sec31/Vp137 from Fred Gorelick and Chris Shugrue (Yale University, New Haven, CT); rabbit anti-p58 ERGIC serum and monoclonal from Jaakko Saraste (University of Bergen, Norway); rabbit anti-GLUT4 (P-1; Ploug et al., 1990 (link)) from Thorkil Ploug (University of Copenhagen, Denmark) and rabbit anti-tubb6 (Randazzo et al., 2019 (link)) from Jim Ervasti (University of Minnesota-Twin Cities, Minneapolis, MN). Mouse anti-GM130 was purchased from BD Biosciences (Franklin Lake, NJ), rabbit anti-GM130, anti-calsequestrin, and anti-α-tubulin from Abcam (Cambridge, MA), mouse anti-α-tubulin DM1A and rabbit anti-p58 from Sigma-Aldrich (St. Louis, MO), rat anti-α-tubulin from Novus Biologicals (Centennial, CO).
Oddoux S., Randazzo D., Kenea A., Alonso B., Zaal K.J, & Ralston E. (2019). Misplaced Golgi Elements Produce Randomly Oriented Microtubules and Aberrant Cortical Arrays of Microtubules in Dystrophic Skeletal Muscle Fibers. Frontiers in Cell and Developmental Biology, 7, 176.
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6

Antibody Staining of Drosophila Wing Discs

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Antibody staining of wing imaginal discs or cells was performed using standard protocols. The following primary antibodies were used: rat anti-Bip (MAC143, Abcam), mouse anti-Wg (4D4; DSHB), guinea pig anti-Sens31 (link), rabbit anti-Wls21 (link), rabbit anti-GFP Alexa Fluor 488 (Molecular Probe), mouse anti-lacZ (Abmart), Rat anti-KDEL (Abcam), and Rabbit anti-GM130 (Abcam). The nuclei were stained by Hoechst 33258 (Sigma). The primary antibodies were detected by fluorescent-conjugated secondary antibodies from Jackson ImmunoResearch Laboratories. Confocal images were collected using a Lecia TCS SP5 confocal microscope with 40X/1.25 oil objectives. Adult thoracic bristle and wing images were obtained using a Nikon SMZ1500 microscope. Z-section images were taken with a Zeiss LSM 780 confocal microscope with 40X/1.3 oil objectives for the colocalization analysis. For determination of the Pearson’s correlation coefficient, the JACoP plugin48 (link) for ImageJ was applied. Images were processed with ImageJ and Adobe Photoshop.
Zhang P., Zhou L., Pei C., Lin X, & Yuan Z. (2016). Dysfunction of Wntless triggers the retrograde Golgi-to-ER transport of Wingless and induces ER stress. Scientific Reports, 6, 19418.
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7

Characterizing Hepatitis C Virus Replication

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Daclatasvir (DCV) (Selleck Chemicals) was dissolved in DMSO and diluted in media to the final concentration used for the assays. Wortmannin (Sigma) was dissolved in DMSO and used at the concentrations described in figure legends. Digitonin (Sigma) was dissolved in PBS and made fresh before each use.
Primary antibodies used include: rabbit anti-GM130 (Abcam Cat. No: ab52649), mouse anti-PI4P (Echelon biosciences Cat. No: ZP004), mouse anti-NS5A (9E10: a kind gift from Charles Rice, Rockefeller University), mouse anti-NS5B (Enzo life sciences Cat. No: ALX-803-061), rabbit anti-Actin (Sigma Cat. No: A2066), mouse anti-HA (Covance Cat. No: MMS-101R). Alexafluor-488 conjugated Wheat germ agglutinin (WGA) (Invitrogen) was diluted to a 1mg/ml stock solution in PBS.
Chukkapalli V., Berger K.L., Kelly S.M., Thomas M., Deiters A, & Randall G. (2014). Daclatasvir inhibits hepatitis C virus NS5A motility and hyper-accumulation of phosphoinositides. Virology, 476, 168-179.
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8

Immunofluorescence Staining of Malaria Parasites

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The dyes and primary antisera employed in this study were as follows: DAPI (4′,6-diamidino-2-phenylindole; Life Technologies) (1:50), Rh-WGA (Vector Labs) (1:40), Nile red (Sigma) (1:50), phalloidin-Alexa 488 (Life Technologies) (1:10), chicken anti-GFP (Abcam ab13990), mouse anti-CSP (gift from Photini Sinnis), rabbit anti-mtTFA (Santa Cruz H-203), rabbit anti-TRAP (gift from Photini Sinnis), rabbit anti-CC3 (catalog no. 9661; Cell Signaling), AAPP (gift from Hiroyuki Matsuoka), and rabbit anti-GM130 (Abcam ab30637). Secondary antibodies (goat anti-rabbit 488 [A11008] and goat anti-mouse 647 [A21235]) were from Life Technologies.
Wells M.B, & Andrew D.J. (2019). Anopheles Salivary Gland Architecture Shapes Plasmodium Sporozoite Availability for Transmission. mBio, 10(4), e01238-19.
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9

Immunofluorescence Staining of Adherent Cells

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Cells grown on poly-l-lysine coated coverslips were washed with PBS twice and fixed with 4% PFA (Biosharp) at room temperature for 15 minutes. Coverslips were washed with PBS 3 times and then blocked with buffer (5% FBS and 0.2% Triton X-100 in PBS) for 30 minutes, and then incubated with primary antibodies diluted in blocking buffer for overnight at 4°C. On the next day, coverslips were washed with PBS 3 times and incubated with fluorescent secondary antibodies and DAPI (4083; CST) for 1 hour at room temperature. Fluorescence was visualized by LSM900 confocal microscope (Zeiss) using a 63 × oil objective. Co-localization analysis was performed using the Pearson correlation coefficient method in Fiji.
The following antibodies were used for immunofluorescence: rat anti-HA (3F10; Roche), mouse anti-FLAG (F1804; Sigma), chicken anti-GFP (A10262; ThermoFisher), rabbit anti-GM130 (52648; Abcam), Alexa fluor 488 donkey anti-chicken (A78948; ThermoFisher), Alexa fluor 633 goat anti-rat (A-21094; ThermoFisher), Alexa fluor 594 donkey anti-rabbit (R37119; ThermoFisher), and Alexa fluor 488 donkey anti-mouse (A-21202; ThermoFisher).
Liu M., Dai E., Yang M., Li S., Fan L., Liu Y., Xiao H., Zhao P, & Yang Z. (2024). Investigating the Impact of Dimer Interface Mutations on Norrin's Secretion and Norrin/β-Catenin Pathway Activation. Investigative Ophthalmology & Visual Science, 65(3), 31.
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10

Immunofluorescence and Immuno EM Protocol

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Immunofluorescence: Mouse anti-syndecan-4 (1:50 dilution, 5G9, SC-12766), goat anti-EEA1 (1:200 dilution) and goat anti-Lamin A/C (1:50 dilution, N-19) were from Santa Cruz Biotechnologies Inc. (Santa Cruz, CA, USA). Rabbit anti-GM130 (1:200 dilution) and rabbit anti-desmin (1:80 dilution) were from Abcam (Cambridge, UK). Mouse anti-HA antibody (1:100 dilution,), Alexa 488 goat anti-mouse, Alexa 546 goat anti-mouse, Alexa 488 goat anti-rabbit and Alexa 647-conjugated donkey anti-goat were from Invitrogen (Carlsbad, CA, USA). DyLight 549-conjugated mouse anti-rabbit were from Jackson ImmunoResearch Laboratories Inc. (West Grove, PA, USA). DAPI was from Molecular probes (Invitrogen, Paisley, UK). Immuno EM: Mouse anti-HA (12CA5, 1:250 dilution) was from Life science Roche (Penzberg, Germany), and rabbit anti-mouse (1:175 dilution) was from Cappel Research Reagents (ICN Biochemicals, Irvin, CA, USA).
Rønning S.B., Carlson C.R., Stang E., Kolset S.O., Hollung K, & Pedersen M.E. (2015). Syndecan-4 Regulates Muscle Differentiation and Is Internalized from the Plasma Membrane during Myogenesis. PLoS ONE, 10(6), e0129288.
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