Abi 3500dx genetic analyzer
The ABI 3500DX Genetic Analyzer is a capillary electrophoresis instrument designed for DNA sequencing and fragment analysis applications. It utilizes fluorescence detection technology to analyze nucleic acid samples.
Lab products found in correlation
45 protocols using abi 3500dx genetic analyzer
Screening Hereditary Hearing Loss Mutations
CRISPR1 Locus Amplification and Sequencing
CRISPR1 locus sequencing from purified PCR products was then performed by the Sanger sequencing technique on an ABI 3500 Dx genetic analyzer (Applied Biosystems, Thermo Fisher Scientific), using CRISPR1-SEQ-F and CRISPR1-SEQ-R internal sequencing primers and BigDyeTerminator mix v3.1 (Applied Biosystems) as previously described (19 (link)).
Evaluating Variant Effects on mRNA
Measles Virus N-450 Genotyping
Whole Exome Sequencing and Variant Analysis
Variants were described according to the nomenclature recommended by the Human Genome Variation Society (
Multiplex Fluorescent PCR for MSI Profiling
Tumors with instability at two or more of these 6 markers were defined as MSI-H, while those without instability or showing instability at only one marker were classified as MSS and Low- Microsatellite Instability(MSI-L) tumors, respectively.
Sanger Sequencing of Regulatory Region
Targeted Sequencing of Deafness Genes
TCOF1, POLR1D, and POLR1C were screened in the probands by targeted gene capture and sequencing of the 121 deafness‐related genes by MyGenostics Corporation (MyGenostics GenCap Enrichment technologies). The data analysis and bioinformatics analysis procedures were performed as previously described.
T-cell Clonality Analysis by PCR
Microsatellite Instability Profiling in Tumor-Normal Pairs
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