AuCl4H2O, sodium citrate, hypoxanthine, hydroquinone, 10 nm gold colloid suspensions (6 × 1012 /mL) and hypoxanthine were purchased from Sigma Aldrich (Ireland). Sterile filtered human serum from normal mixed pool (off the clot) was purchased from TCS Biosciences (Ireland). Uric acid was purchased from FUJIFILM Wako Pure Chemical Corporation.
Five saliva and five urine samples were collected from volunteers from the Technological University, Dublin. The participants were informed that the study is scientifically relevant and not invasive, and their actions would contribute to developing a cheap, fast and simple method, which will improve population health. Verbal informed consent was provided by the participants, who agreed to contribute anonymously.
Commercially available centrifugal filtering devices, Amicon Ultra-0.5 mL (Millipore—Merck, Darmstadt, Germany), with cut-off points at 3 kDa, were employed in this study, as examples.
Five saliva and five urine samples were collected from volunteers from the Technological University, Dublin. The participants were informed that the study is scientifically relevant and not invasive, and their actions would contribute to developing a cheap, fast and simple method, which will improve population health. Verbal informed consent was provided by the participants, who agreed to contribute anonymously.
Commercially available centrifugal filtering devices, Amicon Ultra-0.5 mL (Millipore—Merck, Darmstadt, Germany), with cut-off points at 3 kDa, were employed in this study, as examples.