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Newborn calf serum

Manufactured by Corning

Newborn calf serum is a cell culture supplement derived from the blood of newborn calves. It contains a complex mixture of proteins, growth factors, and other nutrients that support the growth and development of cells in vitro.

Automatically generated - may contain errors

2 protocols using newborn calf serum

1

Routine Culturing of Breast Cancer Cells

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For routine culturing, human breast cancer HCC1806 and MDA-MB-231 cells were obtained from China Infrastructure of Cell Line Resources, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, and MDA-MB-231 polyploid cells (T-MDA-MB-231) were established in the laboratory. These cells were cultured in RPMI-1640 medium (Corning Inc.) containing 10% newborn calf serum (Corning, Inc.) at 37°C and 5% CO2. Cells in the logarithmic growth phase were used in the subsequent experiments.
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2

In vitro Pancreatic Tissue Treatment

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In vitro studies were performed on human pancreatic tissue obtained from NIH/JDRF sponsored integrated islet distribution program or from collaborators. They were obtained after removal of islet cells. They were treated with dh404 at preconditioned concentrations dissolved in DMSO (Sigma, St. Louis, MO) and cultured in Roswell Park Memorial Institute (RPMI-1640) solution containing additionally 10% Newborn calf Serum and Penicillin 100U/ml/Streptomycin 50 µg/ml (Corning, Manassas, VA).
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