Rat igg1 κ isotype control ebrg1

Manufactured by Thermo Fisher Scientific

Rat IgG1 κ isotype control (eBRG1) is a laboratory reagent used as a control in flow cytometry and other immunoassays. It is an antibody derived from rat that shares the same isotype (IgG1) and light chain (κ) as other rat monoclonal antibodies, but does not bind to any known antigen.

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Lab products found in correlation

Anti mouse cd11c apc, by Thermo Fisher Scientific (1 mentions) Anti mouse ifn γ apc, by Thermo Fisher Scientific (1 mentions) Mouse igg1 κ isotype control, by Thermo Fisher Scientific (1 mentions) Easysep mouse cd8 t cell isolation kit, by STEMCELL (1 mentions) Anti mouse cd19 apc, by Thermo Fisher Scientific (1 mentions) Easysep mouse nk cell isolation kit, by STEMCELL (1 mentions)

2 protocols using rat igg1 κ isotype control ebrg1

Comprehensive Immune Cell Profiling

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Anti-human CD45 FITC (HI30), anti-human TIGIT APC (MBSA43), anti-human PD-1 PE (MIH4), anti-mouse TIGIT PE (GIGD7), anti-mouse PVR APC (TX56), anti-mouse PD-1 PE (J43), anti-mouse PD-L1 PE (MIH5), anti-mouse CD45 FITC (30-F11), anti-mouse CD3 PerCP-eFluor710 (17A2), anti-mouse CD8α PE (53-6.7) anti-mouse CD49b PE/APC (DX5), anti-mouse CD19 APC (eBio1D3), anti-mouse CD11c APC (N418), anti-mouse CD11b APC (M1/70), anti-mouse Ly-6G(Gr-1) PE- Cyanine7 (RB6-8C5), anti-mouse F4/80 PerCP-Cyanine5.5 (BM8), anti-mouse IFN-γ APC (XMG1.2), mouse IgG1κ isotype control (P3.6.2.8.1), rat IgM isotype control (eBR2M), rat IgG2α κ isotype control (eBR2a) and rat IgG1 κ isotype control (eBRG1) antibodies were purchased from eBioscience. Anti-mouse TIGIT APC (1G9) was purchased from BioLegend. Antibodies anti-asialo-GM1 (catalog 986-10001) (Wako Chemicals GmbH, Germany) and rabbit IgG control (I8140) (Sigma) were used for NK cell depletion. EasySep mouse CD8⁺ T cell isolation kit (catalog 19853) and EasySep mouse NK cell isolation kit (catalog 19855) (STEMCELL) were used for cell sorting.

Zhou X.M., Li W.Q., Wu Y.H., Han L., Cao X.G., Yang X.M., Wang H.F., Zhao W.S., Zhai W.J., Qi Y.M, & Gao Y.F. (2018). Intrinsic Expression of Immune Checkpoint Molecule TIGIT Could Help Tumor Growth in vivo by Suppressing the Function of NK and CD8+ T Cells. Frontiers in Immunology, 9, 2821.

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Immunohistochemical Quantification of F4/80+ Macrophages

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VWAT tissue sections (5 mice per group) were stained with mAb anti-F4/80 (1∶500, eBioscience, San Diego, CA, USA). Staining was visualized using the Image J software. The specificity of the staining was verified by replacing each primary antibody by nonspecific IgG (Rat IgG1κ isotype Control (eBRG1) eBioscience).

Makki K., Taront S., Molendi-Coste O., Bouchaert E., Neve B., Eury E., Lobbens S., Labalette M., Duez H., Staels B., Dombrowicz D., Froguel P, & Wolowczuk I. (2014). Beneficial Metabolic Effects of Rapamycin Are Associated with Enhanced Regulatory Cells in Diet-Induced Obese Mice. PLoS ONE, 9(4), e92684.

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