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Potassium phosphate

Manufactured by Carl Roth
Sourced in Germany

Potassium phosphate is a chemical compound with the formula K3PO4. It is a crystalline solid that is highly soluble in water. Potassium phosphate is commonly used in various laboratory applications, including as a pH buffer, nutrient source, and in the preparation of other chemical compounds.

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2 protocols using potassium phosphate

1

Lipid Extraction and Quantification

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Tissues were sonicated twice for 10 s on ice in lysis buffer (100 mM potassium phosphate (Carl Roth, Karlsruhe, Germany), 250 mM sucrose (Carl Roth, Karlsruhe, Germany), 1 mM EDTA (ThermoFisher Scientific, Waltham, MA, USA), pH 7) and the protein amount was determined after centrifugation as described above. Lipids were extracted from 1 mg protein following Folch’s method [48 (link)]. TG, TC, and FC concentrations were determined using enzymatic kits following the manufacturer’s guidelines (DiaSys, Holzheim, Germany). CE concentrations were calculated by subtraction of FC from TC values. All lipid concentrations were normalized to protein content.
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2

Electrochemical Biosensor Development

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Chitosan, ferrocene methanol (MeOHFc), horseradish peroxidase, and phenyl methyl sulfonyl fluoride (PMSF) were purchased from Sigma. 1,4-Benzoquinone (1,4-BQ) and ferrocenium hexafluorophosphate (FcPF6) were obtained from Aldrich. d-glucose, potassium dihydrogen phosphate, potassium phosphate, ammonium sulfate, and NaCl were purchased from Roth. Imidazole was obtained from AppliChem; 2,6-dichlorophenol indophenol (DCPIP) from Fluka; ABTS [2,2′-azinobis(3-ethylbenzthiazolinesulfonic acid)] from Amresco; and glutaraldehyde (GA) from Merck.
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