For all animals, a 5-0 braided silk ligature (PERMA-HAND, Ethicon, Somerville, NJ, USA) was placed around the upper left secondary molar. Ligatures are thought to facilitate local accumulation of bacteria and thereby enhance bacteria-mediated inflammation and bone loss (24 (link)). Ligature placement was performed under general anesthesia. Ketamine-xylazine in sterile saline was injected once intraperitoneally at a dose of 100 mg/kg ketamine and 10 mg/kg xylazine. The ligature placement procedure took 5 to min per animal using a surgical microscope and metal clamps to keep the mouths open. While the right maxillary side was left untreated, ligatures where kept in place for 9 d. Eating and drinking behavior was monitored and did not change in the mice following ligature placement.
Perma hand
Manufactured by Johnson & Johnson
Sourced in United States
PERMA-HAND is a lab equipment product developed by Johnson & Johnson. It is designed to provide a secure and durable grip for various laboratory tasks. The core function of PERMA-HAND is to assist users in handling and manipulating laboratory equipment and samples with enhanced control and precision.
Automatically generated - may contain errors
Lab products found in correlation
Vicryl, by Johnson & Johnson (1 mentions)
Monocryl, by Johnson & Johnson (1 mentions)
Ethilon, by Johnson & Johnson (1 mentions)
Ethibond excel, by Johnson & Johnson (1 mentions)
Ti cron, by Medtronic (1 mentions)
Stereotaxic frame, by Kopf Instruments (1 mentions)
Bepanthen ointment, by Bayer (1 mentions)
Ketamin, by Ceva (1 mentions)
4 0 suture, by Johnson & Johnson (1 mentions)
Opsite spray, by Smith & Nephew (1 mentions)
Ketalar, by Pfizer (1 mentions)
8 protocols using perma hand
1
Ligature-Induced Periodontal Disease Model
2
In utero Electroporation of Fmr1 Mice
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Timed pregnant female C57BL/6 Fmr1 HET mice were in utero electroporated as described previously (Fig. 1a ; Suresh and Dunaevsky, 2017 (link)). Briefly, embryonic day (E)15.5 timed pregnant C57BL/6 Fmr1 HET mice were injected with Buprenorphine (0.1 mg/kg) 30 min before the surgery. Following this, the dams were anaesthetized using an isoflurane-oxygen mixture (induction: 5% isoflurane/2 l/min O2, maintenance: 2% isoflurane/2 l/min O2). A small incision was made within the abdominal walls and uterine horns were exposed. 0.5 μl of a 4 μg/μl DNA solution of pCAG-tdTomato and pUB-SEP-GluA2-WPRE was injected into the cerebral lateral ventricles of E15.5 mouse embryo using a pulled glass pipette (BF100-94 Sutter Instrument) and Parker Picospritzer III microinjection system. The head was then placed between 3-mm tweezer electrodes so as to target the motor cortex. Electroporation was achieved using five square pulses (5 ms long at 1 Hz, 35 mV) using BTX Harvard Electro Square Porator ECM 830. Embryos were returned back into the abdominal cavity, and the abdominal muscles were sutured using non absorbable sutures (Ethicon Permahand). The dams were revived and monitored for distress over a period of 24 h after surgery. Dams were allowed to deliver naturally.
3
Suture Materials Characterization Protocols
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Seven suture materials (VICRYL®, MONOCRYL®, Ethilon®, Perma-Hand®, Ethibond EXCEL®, (Ethicon Inc.®, New Jersey, USA), Ti-Cron® (Covidien®, Dublin, Republic of Ireland) and FiberWire® (Athrex®, Florida, USA)) were sourced from our local hospital. Suture materials were obtained pre-sterilised by gamma-irradiation and packaged for clinical use. Respective sutures were cut into homogenous 5mm sections, placed into 24-well tissue culture plates, and pre-soaked in Roswell Park Memorial Institute (RPMI) cell media (Invitrogen™, ThermoFisher Scientific, Australia) for 24 hours. Sterile conditions were maintained in class II laminar flow hoods.
4
Mechanical Stretch Apparatus for Cultured CAM
5
Stereotaxic Viral Particle Injection in Mice
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Mice were placed in a stereotaxic frame (Kopf Instruments) and anesthetized with 1.2 to 2 vol% isoflurane in medical oxygen at a flow rate of 0.8 l/min while keeping body temperature at 39°C. Carprofen (CP-Pharma) was administered subcutaneously (5 mg/kg) and lidocaine (Xylocaine 1%, Aspen Pharma) was injected under the scalp and around ear bars for local anesthesia. Eyes were covered with Bepanthen ointment (Bayer) to keep them lubricated during the surgery. After ensuring the absence of tail- and toe-pinch reflexes, the skin was opened with a midline incision, the periosteum and aponeurotic galea were removed to visualize the reference points bregma and lambda, and the head was aligned to the stereotaxic frame. Small craniotomies were drilled above the injection sites and viral particle solutions were injected with calibrated glass micropipettes (Blaubrand; IntraMARK) at the following coordinates relative to bregma (AP, ML) and pia (DV), summarized in Table 3 .
After viral particle injections, the pipette was left in place for 10 min to allow diffusion and was then slowly retracted. Finally, the skin above the skull was sutured with silk (PermaHand, 6–0; Ethicon), and the animal was transferred to its home cage.
After viral particle injections, the pipette was left in place for 10 min to allow diffusion and was then slowly retracted. Finally, the skin above the skull was sutured with silk (PermaHand, 6–0; Ethicon), and the animal was transferred to its home cage.
6
Tumor Resection and Metastasis Analysis
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Tumor bearing mice were operated the 4th (D4) or 11th day (D11) after the end of the RT treatment or when tumor volume reaches 400mm³ under anesthesia with xylazine (75mg/kg, VMD, Anedonk, Belgium) and ketamin (10mg/kg, CEVA, Brussels, Belgium). Tumors were carefully removed and surgical resection included a margin of 3 mm of healthy tissue. Skin was suturated with 5-0 silk (Perma-hand, Ethicon). Tumor fragments were formol-fixed and paraffin-embedded or frozen in air phase of liquid nitrogen for protein and RNA extractions. For hypoxic area detection, mice were injected intraperitoneally with 100 mg/Kg of Pimonidazole (Hydroxyprobe-1, Chemicon) one hour before tumor resection. After surgery, mice were kept alive until D45 [18 (link)]. At sacrifice, lungs were formol-fixed and paraffin-embedded. Six lung sections of 5 μm, spaced by 10 sections of 5 μm, were immunostained with an antibody against human Ki67 as previously described [18 (link)]. Metastases were manually counted and classified according to their size (<10 cells, 10 to 50 cells, 50 to 100 cells, >100 cells).
7
Carotid Artery Injury Protocol
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All mice underwent transluminal wire injury of the left common carotid artery (CCA) with a 0.36 mm flexible guide wire (Hi-Torque balance middleweight, Abbot, 1001780-HC). The left CCA was exposed via a midline incision at the ventral side and the bifurcation located. Silk ligatures (6-0, PERMA-HAND, Ethicon) were placed around the external carotid artery and distally around the CCA. Two sutures were placed around the internal carotid artery, which was then tied off with the distal ligature. An incision hole was made between the two ligatures and the guidewire was introduced via the incision hole into the CCA. Denudation was achieved by 3 passes along the vessel and 3 rotational passes. The proximal ligature was tied off and blood flow restored. The skin incision was closed using 4-0 sutures.
Animals were euthanized 4–6 weeks after wire injury. Mice were anaesthetized with Zoletil (0.01 mL/g), bled from the orbital plexus and perfused with cold saline. Carotids were cut in two and the upper section was divided into two pieces. The upper piece was fixed in 4% formaldehyde for 30 minutes, transferred to cryoembedding media (Tissue-Tek® O.C.T.) and frozen. The lower part was snap frozen for RNA analysis.
Animals were euthanized 4–6 weeks after wire injury. Mice were anaesthetized with Zoletil (0.01 mL/g), bled from the orbital plexus and perfused with cold saline. Carotids were cut in two and the upper section was divided into two pieces. The upper piece was fixed in 4% formaldehyde for 30 minutes, transferred to cryoembedding media (Tissue-Tek® O.C.T.) and frozen. The lower part was snap frozen for RNA analysis.
8
Autologous Fat Grafting in Rats
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All subjects in the groups were anesthetized with intramuscular ketamine hydrochloride (Ketalar, Pfizer, NY) at a dose of 45 mg/kg and xylazine hydrochloride (Basilazin, Bavet, Germany) at a dose of 5 mg/kg. Ten minutes after the induction of anesthesia, the rats were placed propitiously, and the inguinal area was cleaned with povidone-iodine (Betadine10%, Mundipharma, Germany) and shaved to harvest the graft aseptically (►Fig. 1). Thereupon, the nape regions of rats were shaved (►Fig. 2). To attain the inguinal fat tissue, a 2 to 3 cm skin incision was made in the right groin parallel to the inguinal ligament and the inguinal adipofacial pad was found and dissected from the circumambient tissues (►Fig. 3). Epigastric inferior artery and vein attached to the fat pad were cauterized for hemostasis and fat pad was harvested (►Fig. 4). Adipose tissues taken as grafts weighted between 0.4 and 0.8 g. The surgical region was sutured with a 4/0 suture (Perma Hand, Ethicon Inc., NJ). The fat graft was positioned into the subcutaneous pocket prepared on the nape of the animal (►Fig. 5). The nape incision was sutured with 5/0 suture (Perma Hand®, Ethicon Inc.) (►Fig. 6). The incisions were dressed with OpSite ® spray (Smith & Nephew, UK).
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