EC109 and EC9706 cells were seeded in 6-well plates and transfected with miR-195 mimics, miR-control, anti-miR-195, anti-miR-control, pcDNA-HMGA2, pcDNA empty vector, si-HMGA2, or si-control (GenePharma, Shanghai, China) by Lipofectamine™ 2000 (Invitrogen, Carlsbad, CA, USA) following the manufacturer's protocol when the cells were grown to 80% confluency.
Anti mir 195
Manufactured by GenePharma
Sourced in China
Anti-miR-195 is a laboratory product designed to target and inhibit the expression of the miR-195 microRNA. It functions as an antisense oligonucleotide that binds to and neutralizes miR-195, a small non-coding RNA molecule involved in various cellular processes.
Automatically generated - may contain errors
Lab products found in correlation
Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)
Mir control, by GenePharma (1 mentions)
Si control, by GenePharma (1 mentions)
Pcdna hmga2, by GenePharma (1 mentions)
Si hmga2, by GenePharma (1 mentions)
Pcdna empty vector, by GenePharma (1 mentions)
Anti mir control, by GenePharma (1 mentions)
Dulbecco s modified eagle medium, by Thermo Fisher Scientific (1 mentions)
Dharmafect 4 transfection reagent, by Horizon Discovery (1 mentions)
Pcdna vector, by Promega (1 mentions)
Fetal bovine serum (fbs), by Takara Bio (1 mentions)
Anti mir con, by GenePharma (1 mentions)
Mir con, by GenePharma (1 mentions)
2 protocols using anti mir 195
1
Regulation of HMGA2 by miR-195
2
Investigating miR-195 and OSBP in Cancer Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Human SW872 and 93T449 cells (American Type Culture Collection, ATCC, Manassas, VA, USA) were grown in Dulbecco’s Modified Eagle Medium (Gibco, Carlsbad, CA, USA) containing 10% fetal bovine serum and 1% antibiotics (TaKaRa, Dalian, China). All cells were maintained in a 37°C, 5% CO2 incubator. miR-195 (GenePharma, Shanghai, China), and its corresponding scrambled oligonucleotide sequence (miR-con, GenePharma), anti-miR-195 (GenePharma) and its corresponding scrambled oligonucleotide sequence (anti-miR-con, GenePharma) were transfected into cells using Dharma FECT 4 transfection reagent (Dharmacon, Brebières, France), referring to the protocols of manufacturers. The full sequences of OSBP were inserted into the pcDNA vector (Promega, Southampton, UK) to construct OSBP overexpression plasmid (OSBP), and nontarget vector (pcDNA) was used as a negative control.
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