Protein g agarose beads

Manufactured by Abcam

Sourced in United Kingdom

Protein G-agarose beads are a solid-phase matrix used for the purification and isolation of antibodies from biological samples. Protein G is a bacterial-derived protein that binds to the Fc region of immunoglobulins, allowing antibodies to be captured and separated from other components in the sample.

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Lab products found in correlation

Protein g agarose bead, by Thermo Fisher Scientific (2 mentions) Sirt3, by Santa Cruz Biotechnology (1 mentions) Protein g agarose bead, by Santa Cruz Biotechnology (1 mentions) Ab36764, by Abcam (1 mentions) Ab10812, by Abcam (1 mentions) Protease inhibitor cocktail, by Roche (1 mentions) Immunoprecipitation lysis buffer, by Beyotime (1 mentions) Notch1 antibody, by Abcam (1 mentions) Anti upa antibody, by Abcam (1 mentions) Ip lysis buffer, by Beyotime (1 mentions)

Close spelling variants of this product

Protein G-agarose Protein G beads Agarose beads

5 protocols using protein g agarose beads

Immunoaffinity Assay of SIRT3, p53 and PTEN

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SIRT3 was immunocaptured using antibodies against SIRT3 cross-linked to protein G-agarose beads (Santa Cruz Biotechnology, CA). The immunocomplexes were analyzed by Western blotting and probed with antibodies against p53 (Ab-6) and MDM2.
Wt-p53 was immunocaptured using p53 (Ab-6) cross-linked to protein G-agarose beads (Santa Cruz Biotechnology, CA). The immunocomplexes were analyzed by Western blotting and probed with antibody against MDM2.
Full-length SIRT3 was immunocaptured from nuclear extracts using antibodies against full-length SIRT3 (Abcam Ltd., HK, China) cross-linked to protein G-agarose beads. The PTEN protein was analyzed by Western blotting and probed with anti-PTEN antibody.
PTEN was immunocaptured from nuclear extracts using antibodies against PTEN cross-linked to protein G-agarose beads. The acetylated PTEN was analyzed by Western blotting and probed with acetylated-lysine antibody.

Zhao K., Zhou Y., Qiao C., Ni T., Li Z., Wang X., Guo Q., Lu N, & Wei L. (2015). Oroxylin A promotes PTEN-mediated negative regulation of MDM2 transcription via SIRT3-mediated deacetylation to stabilize p53 and inhibit glycolysis in wt-p53 cancer cells. Journal of Hematology & Oncology, 8, 41.

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Co-Immunoprecipitation of Cellular Proteins

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Five μl of antibody was added to 500 μg of isolated cellular protein within lysis buffer and incubated at 4° C overnight.(29 ) Thirty μl of 50% slurry of pre-washed protein G-agarose beads (Abcam, San Diego, CA) were then added to each sample, followed by incubation for an additional 2 h at 4° C. The samples were spun at 14,000 rpm and washed 4 times in lysis buffer. Samples were then resuspended in 30 μl of lysis buffer for future analysis.

Zhang X., Howell G.M., Guo L., Collage R.D., Loughran P.A., Zuckerbraun B.S, & Rosengart M.R. (2014). CaMKIV-dependent preservation of mTOR expression is required for autophagy during LPS-induced inflammation and acute kidney injury. Journal of immunology (Baltimore, Md. : 1950), 193(5), 2405-2415.

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Piwil2 and H3K9ac Immunoprecipitation

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Cells were lysed with RIPA lysis buffer supplemented with protease inhibitor cocktail (04693124001, Roche, Germany). For Piwil2-IP, cell lysates were incubated with 2 μg of Piwil2-specific antibodies (ab36764, Abcam) or normal rabbit immunoglobulin G (IgG) as a negative control. For H3K9ac-IP, cell lysates were incubated with 2 μg of H3K9ac-specific antibodies (ab10812, Abcam) or normal rabbit IgG as negative control. After an overnight incubation at 4 °C, Protein G Agarose Beads were added (193258, Abcam) and continually incubated for 2 h. Immunoprecipitated proteins were then subjected to SDS-PAGE and western blot analysis.

Feng D., Yan K., Zhou Y., Liang H., Liang J., Zhao W., Dong Z, & Ling B. (2016). Piwil2 is reactivated by HPV oncoproteins and initiates cell reprogramming via epigenetic regulation during cervical cancer tumorigenesis. Oncotarget, 7(40), 64575-64588.

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Notch1 Immunoprecipitation Protocol

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Immunoprecipitation was conducted with protein G agarose beads (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer's instructions. Cells after transfection were lysed and incubated in immunoprecipitation lysis buffer (Beyotime) for 10 min at room temperature. The extracts were incubated with Notch1-antibody (2 μg/ml, Abcam, Cambridge, UK) at 4 °C overnight, and the immunoprecipitate was purified by protein G agarose beads with gentle rocking. The beads were washed for three times with extraction buffer and resuspended in 20 μl SDS loading buffer. The whole cell lysates and immunoprecipitates were incubated at 70 °C for 10 min followed with western blot analysis.

Yang Y., Zhang D., Qin H., Liu S, & Yan Q. (2019). poFUT1 promotes endometrial decidualization by enhancing the O-fucosylation of Notch1. EBioMedicine, 44, 563-573.

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Immunoprecipitation of Urokinase-Type Plasminogen Activator

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IP was conducted with protein G agarose beads (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s instructions. Cells after transfection were lysed and incubated in IP lysis buffer (Beyotime) for 10 min at room temperature. The extracts were incubated with anti-uPA-antibody (2 μg/ml, Abcam, Cambridge, UK) at 4 °C overnight, and the immunoprecipitants were purified by protein G agarose beads with gentle rocking. The beads were washed for three times with extraction buffer and resuspended in 20 μl SDS-loading buffer. The whole cell lysates and immunoprecipitants were incubated at 70 °C for 10 min followed with western blot analysis.

Zhang D., Yang Y., Liang C., Liu J., Wang H., Liu S, & Yan Q. (2019). poFUT1 promotes uterine angiogenesis and vascular remodeling via enhancing the O-fucosylation on uPA. Cell Death & Disease, 10(10), 775.

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