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Kasumi 1 cells

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Kasumi-1 cells are a cell line derived from the peripheral blood of a patient with acute myeloid leukemia. They are a suspension cell line and can be used for research purposes.

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Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions) Hl 60 cells, by American Type Culture Collection (2 mentions) Rpmi 1640, by Thermo Fisher Scientific (2 mentions) Facsaria, by BD (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) Recombinant human wnt3a, by R&D Systems (1 mentions) Fetal bovine serum (fbs), by Merck Group (1 mentions) Indomethacin, by Merck Group (1 mentions) Vitamin d3, by Merck Group (1 mentions) Rpmi 1640, by Lonza (1 mentions) Icg 001, by Selleck Chemicals (1 mentions) Cytarabine, by Abcam (1 mentions) Gene pulser xcell system, by Bio-Rad (1 mentions) Mmessage mmachine t7 kit, by Thermo Fisher Scientific (1 mentions) Mega clearance kit, by Thermo Fisher Scientific (1 mentions) 0.4 cm cuvettes, by USA Scientific (1 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions) Molm 13, by Leibniz Institute DSMZ (1 mentions) Mv4 11, by Leibniz Institute DSMZ (1 mentions) Rapamycin, by Selleck Chemicals (1 mentions)

Spelling variants of this product

Kasumi-1 (131 citations) Kasumi (8 citations)

10 protocols using kasumi 1 cells

1

Culturing and Modulating Hematopoietic Cell Lines

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Kasumi-1 cells (ATCC, Manassas, VA) were cultured in RPMI-1640 (Lonza, Walkersville, MD) supplemented with 10% FBS (Sigma, St Louis, MO) and 1% Penicillin/Streptomycin (Invitrogen, Carlsbad, CA). HL60 cells (ATCC) were cultured in MEMα (Invitrogen) supplemented with 20% FBS and 1% Penicillin/Streptomycin. All cells were maintained at 37 °C and 5% CO2 at concentrations of 106 cells/mL. When indicated, cells were also cultured with the following: 10 uM all-trans retinoic acid (Sigma), 50 uM indomethacin (Sigma), 100 uM cytarabine (Abcam, Cambridge, MA), 0.1 uM vitamin D3 (Sigma), 10 nM recombinant human Wnt3a (R&D Systems, Minneapolis, MN), or 10 nM ICG-001 (Selleck Chemical, Houston, TX). Non-targeting scramble control and TLE4-specific shRNA constructs were developed and delivered to cells via lentiviral delivery as previously described [10 (link)]. The shRNA used and their target sequences were: shTLE4 1 (AGTGATGACAACTTGGTGG) and shTLE4 2 (GGCATTATGTCATGTATTA). Data in figures were obtained using shTLE4 2 unless otherwise indicated. Infected cells were identified by GFP fluorescence detected using FACS LSRII or selected for via cell sorting with FACS Aria (BD, San Jose, CA). Full-length TLE4 (a.k.a.KIAA1261 kind gift of Dr. Ohara [18 (link)]) cDNAs were cloned into the MSCV-IRES-GFP retroviral vector.
Shin T.H., Brynczka C., Dayyani F., Rivera M.N, & Sweetser D.A. (2016). TLE4 regulation of wnt-mediated inflammation underlies its role as a tumor suppressor in myeloid leukemia. Leukemia research, 48, 46-56.
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2

NR4A1 Gene Overexpression in Kasumi-1 Cells

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Kasumi-1 cells were purchased from ATCC. Cells were maintained in 1640 RPMI with 20% FBS. The plasmids and In-vitro transcribed RNA (IVT-RNA) transfection system have been previously described [15 ]. In-vitro transcription (IVT) was performed per manufacturer’s instructions on linearized plasmid containing the NR4A1 coding sequence using the mMESSAGE mMACHINE® T7 Kit (Applied Biosystems). RNA polyadenylation was performed with a Poly(A) Tailing Kit, and resulting IVT-RNA was purified with a MEGA Clearance Kit (Applied Biosystems) according to manufacturer instructions. For electroporation, cells were suspended to a final concentration of 1 million cells per 100uL DPBS. 200uL of cell solution was transferred to 0.4 cm cuvettes (USA Scientific), mixed by pipetting with IVT-RNA at a final concentration of 100 nM, and immediately electroporated at 330V for 5 milliseconds with the GenePulser Xcell system (Bio-Rad).
Duren R.P., Boudreaux S.P, & Conneely O.M. (2016). Genome Wide Mapping of NR4A Binding Reveals Cooperativity with ETS Factors to Promote Epigenetic Activation of Distal Enhancers in Acute Myeloid Leukemia Cells. PLoS ONE, 11(3), e0150450.
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3

Establishment of Cabozantinib-Resistant AML Cell Lines

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The human AML cell lines Molm13 (DSMZ, Brauschweig, Germany) and MV4-11 (DSMZ) were maintained in Roswell Park Memorial Institute (RPMI)-1640 medium (ThermoFisher Scientific/Gibco, Waltham, MA, USA), containing 10% fetal bovine serum (FBS); Kasumi-1 cells (ATCC, Manassas, VA, USA) were cultured in RPMI-1640 medium containing 20% FBS. The cabozantinib-resistant cell line, Molm13-XR, was established by culturing Molm13 cells in the presence of increasing concentrations of cabozantinib. The stable resistant cell line was then maintained in a complete RPMI-1640 medium with 10% FBS, in the absence of cabozantinib. Cell lines were cultured at 37 °C in a humidified atmosphere containing 5% CO2. The genetic profiles of these cell lines were authenticated (16-markers short tandem repeat) by the Food Industry Research and Development Institute (Hsinchu, Taiwan), and were found to be identical to their reported generic profiles.
Cabozantinib-malate, pyrvinium pamoate, and rapamycin were purchased from Selleck Chemicals (Houston, TX, USA), Sigma-Aldrich/Merck (Darmstadt, Germany), and TargetMol (Boston, MA, USA), respectively.
Fu Y.H., Tseng C.Y., Lu J.W., Lu W.H., Lan P.Q., Chen C.Y., Ou D.L, & Lin L.I. (2021). Deciphering the Role of Pyrvinium Pamoate in the Generation of Integrated Stress Response and Modulation of Mitochondrial Function in Myeloid Leukemia Cells through Transcriptome Analysis. Biomedicines, 9(12), 1869.
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4

AML and CML Cell Samples Protocol

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FLT3(ITD);TET2mut, FLT3(ITD);AML1-ETO and AML1-ETO –positive primary AML samples were from the ECOG-ACRIN E1900 clinical trial (Patel et al., 2012 (link)). JAK2(V617F);TET2mut and BRCA1/2-deficient AML samples were described before (Nieborowska-Skorska et al., 2017a (link), 2017b (link)). BCR-ABL1 –positive CML-CP samples were obtained from the Medical University of Vienna and Ludwig-Boltzmann Institute for Hematology and Oncology, Vienna, Austria. Samples of normal hematopoietic cells were purchased from StemCell Technologies (Vancouver, Canada). Lin−CD34+ cells were obtained from mononuclear fractions by magnetic sorting using the EasySep Lin negative selection cocktail followed by CD34 positive selection (StemCell Technologies) as described before (Nieborowska-Skorska et al., 2017b (link)). CML-BP K562 cells, AML MV4–11 cells and Kasumi-1 cells were from ATCC. TGFβR2 CRISPR/Cas9 knock-down K562-Cr cells were from Applied Biological Materials Inc. (Vancouver, Canada). These leukemic cell lines were maintained in DMEM supplemented with 10% FBS, L-glutamine and antibiotics in 37°C.
Le B.V., Podszywalow-Bartnicka P., Maifrede S., Sullivan-Reed K., Nieborowska-Skorska M., Golovine K., Yao J.C., Nejati R., Cai K.Q., Caruso L.B., Swatler J., Dabrowski M., Lian Z., Valent P., Paietta E.M., Levine R.L., Fernandez H.F., Tallman M.S., Litzow M.R., Huang J., Challen G.A., Link D., Tempera I., Wasik M.A., Piwocka K, & Skorski T. (2020). TGFβR-SMAD3 Signaling Induces Resistance to PARP Inhibitors in the Bone Marrow Microenvironment. Cell reports, 33(1), 108221.
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5

Culturing Kasumi-1 Cells for Research

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Kasumi-1 cells (ATCC) were cultured in RPMI (Gibco) supplemented with 20% fetal calf serum (FCS, HyClone), 2 mM GlutaMAX (Gibco), 100 units/mL penicillin, and 100 μg/mL streptomycin (Gibco). Cells were cultured at 5% CO2 and 37 °C. Cell lines were tested monthly for mycoplasma contamination.
Yashar W.M., Kong G., VanCampen J., Curtiss B.M., Coleman D.J., Carbone L., Yardimci G.G., Maxson J.E, & Braun T.P. (2022). GoPeaks: histone modification peak calling for CUT&Tag. Genome Biology, 23, 144.
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6

Cell Culture Conditions for Leukemia Research

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Cbfb+/56M, Mx1-Cre+ cells were cultured in RPMI-1640 (ATCC, Manassas, Virginia) supplemented with 20% ES cell qualified fetal bovine serum (Thermo Fisher Scientific, Waltham, MA) 1% penicillin/streptomycin, 1% L-glutamine, 10 ng/mL IL-3, 10 ng/mL IL-6, 20 ng/mL SCF (Peprotech, Rocky Hill, NJ) and cryopreserved in RPMI-1640 supplemented with 50% FBS and 10% DMSO. COS-7 cells (ATCC) and HEK293T cells (ATCC) were maintained in DMEM supplemented with 10% fetal bovine serum, 1% penicillin/streptomycin, and 1% L-glutamine. ME-1 cells (kindly provided by P. Liu, NHGRI/NIH) were maintained in RPMI-1640 supplemented with 20% fetal bovine serum, 2.5% of a 10% (w/v) glucose solution, 1% penicillin/streptomycin, 1% L-glutamine, 1% sodium pyruvate, and 2.5% 1M HEPES. Kasumi-1 cells (ATCC) were maintained according to ATCC recommended protocol. Leukemia cell lines were validated by karyotype analysis by the UNMC Human Genetics Laboratory and/or western blot analysis. Cells were maintained in culture for less than 3 months at 37°C, 5% CO2 and routinely monitored for Mycoplasma contamination using MycoAlert PLUS Mycoplasma Detection Kit (Lonza).
Richter L.E., Wang Y., Becker M.E., Coburn R.A., Williams J.T., Amador C, & Hyde R.K. (2019). HDAC1 is a Required Cofactor of CBFβ-SMMHC and a Potential Therapeutic Target in Inversion 16 Acute Myeloid Leukemia. Molecular cancer research : MCR, 17(6), 1241-1252.
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7

Culturing Human Hematopoietic Cell Lines

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Kasumi-1 cells (ATCC CRL-2724), HL60 cells (ATCC CCL-240), THP-1 cells (ATCC TIB-202) and U937 cells (CRL-1593) were purchased from ATCC. Cells were cultured following the manufacturer’s culture method. Kasumi cells were cultured with RPMI-1640 growth medium (Gibco, 11875093) containing 20% FBS. HL-60 cells were cultured with IMDM containing 20% FBS (Thermofisher 12440053). THP-1 cells were cultured with RPMI-1640 containing 10% FBS. U937 cells were cultured with RPMI-1640 containing 10% FBS.
Zhang N., Long Y, & Devreotes P.N. (2001). Gγ in Dictyostelium: Its Role in Localization of Gβγ to the Membrane Is Required for Chemotaxis in Shallow Gradients. Molecular Biology of the Cell, 12(10), 3204-3213.
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8

Establishing CCND2 AML Cell Lines

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KG1a and Kasumi-1 cells (both CCND1 and CCND2 wild-type) were obtained from the American Type Culture Collection (ATCC, Manassas, VA) for studying the effects of the CCND2 Thr280Ala mutation in AML. Both cell lines were authenticated by ATCC using the cytochrome C oxygenase 1 gene (COI) assay for interspecies determination, and short tandem repeat (STR) analysis for intraspecies determination of the unique DNA profiles. The cells were cultured in RPMI medium supplemented with 10% Fetal Bovine Serum (FBS) and 1% Antibiotic-Antimycotic (Gibco, Big Cabin, OK).
Eisfeld A.K., Kohlschmidt J., Schwind S., Nicolet D., Blachly J.S., Orwick S., Shah C., Bainazar M., Kroll K.W., Walker C.J., Carroll A.J., Powell B.L., Stone R.M., Kolitz J.E., Baer M.R., de la Chapelle A., Mrózek K., Byrd J.C, & Bloomfield C.D. (2016). Mutations in the CCND1 and CCND2 genes are frequent events in adult patients with t(8;21)(q22;q22) acute myeloid leukemia. Leukemia, 31(6), 1278-1285.
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9

Cell Line Maintenance and Synchronization

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Kasumi-1 cells were obtained from American Type Culture Collection (ATCC). MV4–11 and K562 cells were gifts from Emery Bresnick. THP-1 cells were a gift from David Beebe. All cell lines were validated at UW-Madison using polymorphic short tandem repeat loci and were free of mycoplasma. All cell lines were maintained at 37°C and 5% CO2 in a humidified incubator in RPMI 1640 medium containing 2 mM L-glutamine, supplemented with 100 units/mL penicillin-streptomycin and 10% (THP-1, K562 and MV4–11) or 20% (Kasumi-1) fetal bovine serum.
For thymidine synchronization, cells were challenged with 2.5 mM thymidine for 24 h, washed once with HBSS, then replenished with normal media for 8 h prior to further chemical treatments.
Jin N., Lera R.F., Yan R.E., Guo F., Oxendine K., Horner V.L., Hu Y., Wan J., Mattison R.J., Weaver B.A, & Burkard M.E. (2020). Chromosomal instability upregulates interferon in acute myeloid leukemia. Genes, chromosomes & cancer, 59(11), 627-638.
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10

Kasumi-1 Cells for AML-M2 Translocation Research

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Kasumi-1 cells (12 (link)), derived from the peripheral blood of a 7 year old Japanese male who was diagnosed with AML-M2, were purchased from the American Type Culture Collection (Manassas, VA, USA) and were maintained in RPMI-1640 with 20% fetal bovine serum (Gibco). The genetic characteristics of this cell line include a chromosome t(8;21) (q22;q22) translocation, thus making it a good research tool for investigating this type of translocation in leukemia. The cell line was incubated in a 37°C humidified atmosphere with 5% CO2. Different concentrations of ZGDHu-1 (50, 100, 200, 500 and 1,000 μg/l) and controls (negative control and DMSO as solvent control) were added to the Kasumi-1 cells.
XIA J., CHEN S.F., LV Y.P., LU L.N., HU W.X, & ZHOU Y.L. (2015). ZGDHu-1 induces G2/M phase arrest and apoptosis in Kasumi-1 cells. Molecular Medicine Reports, 11(5), 3398-3404.
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