Bacteriological Agar powder was purchased from Du Pont de Nemours Int., South Africa and Oxoid Ltd., Basingstoke, Hampshire and England. Naphthalene acetic acid (NAA), Indole-3-butyric acid (IBA), Phloroglucinol (PG), myo-inositol, vitamins (thiamine HCl, nicotinic acid, pyridoxine HCl) and glycine, were obtained from Sigma Aldrich, Germany. meta-Topolin (mT) was prepared as described previously [60 (link),61 (link)]. The compounds 3-(4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium Bromide (Sigma Aldrich, Darmstadt, Germany) Minimal Essential Medium (MEM) (Whitehead Scientific, Winelands Close, Stikland, 7530, South Africa), Gentamicin (Virbac, Carros, France), Foetal Calf Serum (Highveld Biological, Johannesburg, South Africa) and Doxorubicin chloride (Pfizer Laboratories, 85 Bute Lane, Sandton, 2146, South Africa) and Phosphate Buffered Saline (PBS) (Whitehead Scientific, Winelands Close, Stikland, 7530, South Africa) were used. All chemicals used in the assays were of analytical grade.
Naphthaleneacetic acid
Manufactured by Merck Group
Sourced in Germany, United States
Naphthaleneacetic acid is a plant growth regulator used in laboratory settings. It is a synthetic auxin that can influence various physiological processes in plants.
Automatically generated - may contain errors
Lab products found in correlation
Kinetin, by Merck Group (3 mentions)
6 benzylaminopurine, by Merck Group (2 mentions)
Sucrose, by Merck Group (2 mentions)
Indole 3 acetic acid, by Merck Group (2 mentions)
6 benzylaminopurine bap, by Merck Group (2 mentions)
Nicotinic acid, by Merck Group (1 mentions)
Myo inositol, by Merck Group (1 mentions)
Indole 3 butyric acid, by Merck Group (1 mentions)
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide, by Merck Group (1 mentions)
Gentamicin, by Virbac (1 mentions)
Glycine, by Merck Group (1 mentions)
Phloroglucinol, by Merck Group (1 mentions)
Distilled water, by Merck Group (1 mentions)
Picloram, by Merck Group (1 mentions)
Potassium persulfate, by Merck Group (1 mentions)
Agar, by Merck Group (1 mentions)
Aluminum chloride, by Merck Group (1 mentions)
Clorox, by Merck Group (1 mentions)
α naphthalene acetic acid, by Merck Group (1 mentions)
2 2 azino bis 3 ethylbenzothiazoline 6 sulfonic acid, by Merck Group (1 mentions)
12 protocols using naphthaleneacetic acid
1
Preparation and Characterization of Plant Cell Culture Media
2
Phytochemical and Antioxidant Analysis
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All the chemicals used were analytical grade. Clorox (5.25% of sodium hypochlorite), sulfuric acid, sucrose, agar, sodium hydroxide (NaOH), hydrochloric acid (HCl), indole-3-acetic acid (IAA), picloram, α-Naphthalene acetic acid (NAA), 6-Benzylaminopurine (BAP), kinetin (Kin), Naphthalene acetic acid (NAA), ethanol, distilled water, 2, 2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-Azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), quercetin, ascorbic acid, sodium nitrate, aluminum chloride, potassium persulfate, Folin–Ciocalteu reagent and 2,3,5-triphenyl-tetrazolium chloride (TZ), were purchased from Sigma-Aldrich GmbH (Munich, Germany). Free peat (peat moss Holland) soil media were purchased from local nursery. UV-Vis spectrophotometer were used to measure absorbance (UV-Vis. Cary 4000, Agilent, UK). Rotary evaporator (Rotavapor R-200, Switzerland).
3
Quantification of Endogenous Free Salicylic Acid in Rice
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Endogenous free SA levels were determined in rice leaf sheaths. Fifteen leaf sheaths from each plant or line were ground to a fine powder in liquid nitrogen as a replicate. Three biological replicates of each frozen sample were prepared. Each sample (100 mg) was added to 750 μl precooled extraction buffer (methanol:water:acetic acid, 80:19:1, v/v/v) supplemented with 3 µg naphthaleneacetic acid (Sigma-Aldrich, 35745) as an internal standard and shaken at 4 °C overnight. After centrifugation, the supernatant was filtered through a 0.22-μm nylon membrane and dried under a nitrogen flow at room temperature for at least 4 h. Finally, 200 μl methanol was added to dissolve the extracts. Endogenous free SA measurements were performed as described elsewhere55 (link).
4
Arabidopsis Cell Suspension Culture Protocol
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Arabidopsis cell suspension (ecotype Landsberg erecta) was cultured in growth medium consisting of 1× Murashige and Skoog (MS) Modified Basal Salt Mixture (Phyto Technology M524) without vitamins, 3% w/v sucrose (Ajax Chemical A530), 0.5 mg/l naphthaleneacetic acid (Sigma–Aldrich 15165-79-4), 0.05 mg/l kinetin (Sigma–Aldrich K0753) and with a pH of 5.8. Cells were incubated at 22°C with orbital shaking at 100–120 rpm and under constant light (100 µmol m−2 s−1). Cultures were maintained in 250 ml Erlenmeyer flasks by the inoculation of 20 ml of 7 days old cells into 100 ml of fresh growth medium.
5
Polyphenol Analytical Procedures via HPLC
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Reagents of analytical grade used for HPLC analyses included the standards trans-piceid, ɛ-viniferin, and trans-resveratrol (Sigma-Aldrich, St. Louis, MI, USA). Other used reagents were methyljasmonate (MeJA), cis-jasmonate (cJA), dihydrojasmonic acid (2HJA), cellulase from Trichoderma viride (TvC), sodium orthovanadate (NaVO) (Sigma-Aldrich, St. Louis, MI, USA), naphthaleneacetic acid (NAA), 6-benzylaminopurine (BAP), sucrose, agar, yeast extract, bacto-peptone, magnesium sulphate heptahydrate, potassium dihydrogen phosphate, sodium dodecylsulphate (SDS) (Sigma-Aldrich, St. Louis, MI, USA; Duchefa BIOCHEMIE B.V, Netherlands; Imuna, Slovakia, Merck, Germany). Water was purified by the Direct-Q® 3 UV Water Purification System (Merck Millipore, Darmstadt, Germany). Reference standard solutions of polyphenols were prepared in mixture methanol:water (1:1, v/v) and stored at 4 °C in dark.
6
Preparation of Murashige & Skoog Media
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Liquid Murashige & Skoog Minimal Organics (MSMO) media was prepared as follows: sucrose (30 g; Univar), 4-morpholineethanesulfonic acid (MES; 0.5 g; Astral Scientific) and Linsmeier & Skoog Basal Medium (4.4 g; PhytoTech Labs) were dissolved in ca. 900 mL of H 2 O, -naphthaleneacetic acid (500 µL; 1 mg/mL) and kinetin (50 µL; 1 mg/mL; Sigma) were added and the pH of the mixture was adjusted to 5.7 with aqueous KOH before dilution to 1000 mL with H 2 O. The medium was sterilised by autoclaving.
MSMO media containing 15 N was prepared as follows: Murashige & Skoog Modified Basal Salt Mixture (0.6 g; PhytoTech Labs), 15 NH 4 15 NO 3 (1.7 g), K 15 NO 3 (1.9 g), KH 2 PO 4 (0.2 g), myo-inositol (0.1 g), sucrose (30 g; Univar), and MES (0.5 g; Astral Scientific) were dissolved in ca. 900 mL of H 2 O; hormones were added, the pH was adjusted and the mixture was diluted, as described above. The medium was sterilised by autoclaving.
MSMO media containing 15 N was prepared as follows: Murashige & Skoog Modified Basal Salt Mixture (0.6 g; PhytoTech Labs), 15 NH 4 15 NO 3 (1.7 g), K 15 NO 3 (1.9 g), KH 2 PO 4 (0.2 g), myo-inositol (0.1 g), sucrose (30 g; Univar), and MES (0.5 g; Astral Scientific) were dissolved in ca. 900 mL of H 2 O; hormones were added, the pH was adjusted and the mixture was diluted, as described above. The medium was sterilised by autoclaving.
7
Root Growth Assay of Oxylipins and Auxins
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The following compounds were tested in root growth assays using the indicated concentrations. 9-HOT (9(S)-hydroxy-10(E),12(Z),15(Z)-octadecatrienoic acid), 9-KOT (9-keto-10(E),12(Z),15(Z)-octadecatrienoic acid) and OPDA (12-O xo-P hytod ienoic A cid) were prepared as described in [11 (link),12 (link)], respectively. TIBA (2,3,5-triiodobenzoic acid), Naphthalene-Acetic Acid (NAA) and Jasmonic Acid (JA) were obtained from Sigma (Munich, Germany).
8
Glycyrrhiza uralensis Cell Culture
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The experiment was carried out in the plant cell culture room of the Inner Mongolia University of Science and Technology (China). The tested licorice variety was Glycyrrhiza uralensis Fisch, and the seeds were purchased from Erdos City, Inner Mongolia, China. A licorice cell suspension culture system was established, and the entire operation was carried out at 25±1°C, with the pH maintained at 5.8 [5 (link)]. Nicotiana benthamiana was grown in a glasshouse and used for subcellular localization analysis.
Some kits, antibiotics, pUCm-T vectors, and primers used in the experiment were purchased from Sangon Biotech (Shanghai, China). Restriction enzymes were purchased from Takara Biomedical Technology (Beijing, China). T4 DNA Ligase, ampicillin, 6-benzyladenine (6-BA), naphthaleneacetic acid (NAA), 2,4-dichlorophenoxyacetic acid (2,4-D), and MeJA were purchased from Sigma-Aldrich (USA). Escherichia coli DH5α, Escherichia coli Top10, Agrobacterium tumefaciens GV3101, and pJG054 plasmid vectors were all available and stored in the laboratory.
Some kits, antibiotics, pUCm-T vectors, and primers used in the experiment were purchased from Sangon Biotech (Shanghai, China). Restriction enzymes were purchased from Takara Biomedical Technology (Beijing, China). T4 DNA Ligase, ampicillin, 6-benzyladenine (6-BA), naphthaleneacetic acid (NAA), 2,4-dichlorophenoxyacetic acid (2,4-D), and MeJA were purchased from Sigma-Aldrich (USA). Escherichia coli DH5α, Escherichia coli Top10, Agrobacterium tumefaciens GV3101, and pJG054 plasmid vectors were all available and stored in the laboratory.
9
Plant Growth Hormone Assay
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Naphthalene acetic acid (NAA; Sigma-Aldrich) and indole-3-acetic acid (IAA; Sigma-Aldrich) were dissolved with a small volume of 1 N NaOH and then diluted with water to a final concentration of 100mM before filter sterilization. These stock solutions were added together with DEX or EtOH to autoclaved medium at the indicated concentrations. Pre-cultured seedlings were transferred to the media for treatment or seeds were directly germinated on the media.
10
Preparation of n-Alkane and Plant Growth Regulators
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The n-alkane standard (C7-C30) reference material at 1000 μg/mL in hexane was purchased from Sigma-Aldrich (catalog number 49451-U; Castle Hill, NSW, Australia). Naphthalene acetic acid (NAA) (95% purity, product number N0640) was purchased in crystalline form from Sigma-Aldrich (Castle Hill, NSW, Australia). Mohammed et al. [25 (link)] described all other standards and regents. Briefly, these included high-performance liquid chromatography grade ethanol, n-hexane (95%), benzyladenine (BA) (98%), sodium hydroxide as a pellet (97%), rooting hormone (Clonex) in gel form, vapor guard (anti-transpirant concentrate) liquid, deionized water (Milli-Q Ultrapure water system) and double distilled water.
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