The level of GSH was determined using the GSH sensitive probe
Celltracker Green CMFDA (Molecular Probes, Eugene, OR, USA). 1C11 and 1C11
5−HT cells were exposed to TiO
2 or CB nanoparticles (1 µg cm
−2) up to 24 h. The cells were then washed twice with Hanks’ balanced salt solution (
HBSS) buffer (Invitrogen, ThermoFisher Scientific, MA, USA) and further incubated for 30 min at 37 °C in HBSS in the presence of 1 µM fluorogenic reagent. HBSS was removed, and the cells were left to reconstitute in DMEM, 10% FCS for 30 min at 37 °C before lysis. Fluorescence intensity of cell lysates was recorded at λ
em = 517 nm (slit width = 5 nm) after excitation at λ
exc = 492 nm (slit width = 5 nm) using a
Cary Eclipse fluorometer (Varian Inc., Agilent Technology). The reference level of intracellular reduced GSH (100%) was obtained using cells unexposed to nanoparticles.
Ribeiro L.W., Pietri M., Ardila-Osorio H., Baudry A., Boudet-Devaud F., Bizingre C., Arellano-Anaya Z.E., Haeberlé A.M., Gadot N., Boland S., Devineau S., Bailly Y., Kellermann O., Bencsik A, & Schneider B. (2022). Titanium dioxide and carbon black nanoparticles disrupt neuronal homeostasis via excessive activation of cellular prion protein signaling. Particle and Fibre Toxicology, 19, 48.
