Cell lysis and Western blotting were conducted as described (Yaakov et al., 2003 (link)). Anti–phospho-p38 antibody from Cell Signaling Technology (Beverly, MA) was used to detect phosphorylated Hog1. Hog1 (Y-215) antibody from Santa Cruz Biotechnology (Santa Cruz, CA) was used to detect Hog1 protein. The hemagglutinin (HA)-tagged protein was detected by HA antibody 3F10 from Roche.
Hog1 y 215 antibody
Manufactured by Santa Cruz Biotechnology
Sourced in United States
Hog1 (Y-215) antibody is a primary antibody targeting the Hog1 protein. Hog1 is a mitogen-activated protein kinase (MAPK) involved in the high osmolarity glycerol (HOG) signaling pathway. This antibody recognizes Hog1 when phosphorylated at the Tyr215 residue.
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Lab products found in correlation
2 protocols using hog1 y 215 antibody
1
Phosphorylation Assay for Hog1 Kinase
2
Western Blotting for Phospho-Hog1 and MAPK
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Protein extraction and western blotting were performed as previously described [30 (link)]. An anti-phospho-p38 (Thr180/Tyr182) monoclonal antibody (#9211, Cell Signaling Technology, Danvers, MA, USA) and Hog1 (y-215) antibody (sc-9079, Santa Cruz Biotechnology, Inc., Dallas, TX, USA) were used to detect phospho-Hog1 and total Hog1, respectively. Moreover, an anti-p-P44/42 MAPK antibody (4270S, Cell Signaling Technology) was used for the detection of both phosphor-Mkc1 and phosphor-Cek1, and a GAPDH antibody (GTX100118, GeneTex, Hsinchu, Taiwan) was used for GAPDH. Horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG (GTX213110, GeneTex) was used as the secondary antibody. The blot was visualized using the Western Lightning Plus-ECL Enhanced Chemiluminescence Substrate kit (PerkinElmer) and an ImageQuant LAS 4000 Biomolecular Imager (GE Healthcare Life Science, Marlborough, MA, USA).
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