Elecsys t4

Manufactured by Roche

Sourced in Switzerland

The Elecsys T4 is a laboratory instrument used to measure the levels of thyroxine (T4) in blood samples. T4 is a hormone produced by the thyroid gland and is important for regulating metabolism. The Elecsys T4 provides quantitative measurements of T4 concentrations, which can be used to assess thyroid function.

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Lab products found in correlation

Elecsys t3, by Roche (2 mentions) Architect tsh reagent, by Abbott (2 mentions) Modular p800 chemistry analyzer, by Roche (2 mentions) Elecsys tsh, by Roche (1 mentions) Immulite 2000, by Siemens (1 mentions) Elecsys 2010, by Roche (1 mentions) Bca kit, by Solarbio (1 mentions) Ripa cell lysis buffer, by Solarbio (1 mentions)

4 protocols using elecsys t4

Thyroid Function in Nephrotic Syndrome

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The medical records of the patients were retrospectively reviewed. For 31 patients who were diagnosed with NS, serum total protein, albumin, total cholesterol, electrolyte levels, Cr, and estimated glomerular filtration ratio (eGFR) were measured. In addition, thyroid hormone profiles were analyzed. The total 24-hour urinary protein excretion, or early-morning spot urinary protein/Cr ratio was measured.
The serum T3, T4, TSH, and free T4 levels were measured using the electro-chemiluminescence immunoassay (ECLIA) to assess the thyroid function. In the ECLIA, chemiluminescent ruthenium is used to electrically induce antigen-antibody reactions on the surface of steroptoavidin-coated paramagnetic micro-beads, and the luminescence emitted is measured to determine the concentration of the substance of interest. The Elecsys T3, Elecsys T4, Elecsys TSH, and Elecsys FT4 II kits from Roche Diagnostics Co., Ltd. were used. For the normal reference range, the normal range of each kit was used (range corresponding to the 2.5th–97.5th percentiles).
In addition, anti-thyroglobulin, anti-thyroid-stimulating hormone receptor antibody, and anti-thyroid peroxidase antibodies were measured in some patients with overt hypothyroidism.

Jung S.H., Lee J.E, & Chung W.Y. (2018). Changes in the thyroid hormone profiles in children with nephrotic syndrome. Korean Journal of Pediatrics, 62(3), 85-89.

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Postoperative Blood Biomarker Analysis

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Blood extraction were obtained 1‐3 months postoperatively and at the final study visit. Serum samples for biochemical analyses were obtained between 8 and 9 am after overnight fast and immediately kept frozen at −70°C until they were measured by auto analyzer (Modular P800 Chemistry Analyzer, Roche Diagnostic). Serum levels of creatinine, calcium (corrected for albumin binding), and phosphate were measured. Serum TSH (Architect TSH reagent; Abbot Laboratories) and free (T4) by electrochemiluminescence (ElecsysT4, Roche Diagnostic; functional sensitivity <0.01 µg/mL).

Hawkins Carranza F., Guadalix Iglesias S., Luisa De Mingo Domínguez M., Martín‐Arriscado Arroba C., López Álvarez B., Allo Miguel G, & Martínez Díaz‐Guerra G. (2020). Trabecular bone deterioration in differentiated thyroid cancer: Impact of long‐term TSH suppressive therapy. Cancer Medicine, 9(16), 5746-5755.

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Serum Vitamin D and Thyroid Biomarkers

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Fasting serum samples were obtained between 8 and 9 AM in the summer–autumn months and were immediately frozen at −70 °C until the assays were performed. Serum albumin-corrected calcium, phosphate, and creatinine were measured by automated standard laboratory methods (Modular P800 Chemistry Analyzer; Roche Diagnostics, Basil, Switzerland) and 24-h urinary calcium by colorimetric method. Serum 25–hydroxyvitamin D3 (25OHD) levels were measured by enzyme immunoassay (automated IDS EIA, kit, Roche Diagnostics, Basil, Switzerland). Endocrine Society 2011 guidelines categorization values have been used in this study: deficiency < 20 ng/mL; insufficiency ≥ 20 to 29 ng/mL; and sufficiency > 30 ng/mL (12).
Serum TSH was measured by chemiluminescence (Architect TSH reagent; Abbot Laboratories, San Francisco, CA, USA), free thyroxine (fT4) by electrochemiluminescence (Elecsys T4, Roche Diagnostics, Basil, Switzerland) (functional sensitivity < 0.01 µg/mL). Serum intact PTH (normal range: 7–57 pg/mL) was determined using chemiluminescent immunoassays with an Immulite 2000 (Siemens Healthcare, Erlangen, Germany).

Hawkins Carranza F., Guadalix Iglesias S., De Mingo Dominguez M.L., Allo Miguel G., Arroba C.M., Alvares B.L, & Martínez Diaz-Guerra G. (2020). Association of Low Serum 25OHD Levels with Abnormal Bone Microarchitecture in Well-Differentiated Thyroid Cancer. Medical Sciences, 8(4), 49.

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Quantitative Thyroid Hormone Analysis

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The above TH extractions were quantitatively tested using Elecsys T3 and Elecsys T4 kits (Roche Diagnostics, Mannheim, Germany) on an Elecsys 2010 automated electrochemical immuno-analyzer (Roche Diagnostics, Germany) following the manufacturer’s instructions [18 (link)]. TH contents were determined automatically according to the standard curve. A negative control (0.01 M NaOH) was tested in each batch and the results were used for calibration. The detection limits of this system were 5.40 nmol/L for T4 and 0.30 nmol/L for T3 and the lowest value detected were 9.30 nmol/L for T4 and 0.62 nmol/L for T3, respectively. Thus, all detected values were located in convincing range. The recovery of our processing system was assessed by pike in standard T4 and T3 in larval samples before the samples were ground in liquid nitrogen. The mean recovery of standard T4 and T3 was 57.65% ± 1.31% and 27.22% ± 1.68%, respectively. For protein analysis, approximately 0.01 g of dried samples was dissolved for 1 h in 1 mL cell lysis buffer RIPA (Solarbio, Beijing, China) at room temperature, and protein assay was conducted using a BCA kit (Solarbio, China) following the manufacturer’s instructions. The larval content of THs was corrected using the mean recovery values and expressed as microgram THs per gram protein (μg/g protein).

Huang W., Xu F., Qu T., Zhang R., Li L., Que H, & Zhang G. (2015). Identification of Thyroid Hormones and Functional Characterization of Thyroid Hormone Receptor in the Pacific Oyster Crassostrea gigas Provide Insight into Evolution of the Thyroid Hormone System. PLoS ONE, 10(12), e0144991.

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