For the TLC, we used silica gel 60 F254 precoated plates (Merck); for column chromatography (CC), silica gel 60 (70–230 or 230–400 mesh, Merck) and Spherical C18 100A Reversed Phase Silica Gel (RP-18) (particle size: 20–40 μm) (Silicycle). For the HPLC, we used a spherical C18 column (250 × 10 mm, 5μm) (Waters) and LDC-Analytical-III apparatus. For the UV spectra, we used a Jasco UV-240 spectrophotometer, λmax (log ε) in nm. For the optical rotation, we used a Jasco DIP-370 polarimeter, in CHCl3. For the IR spectra, we used a Perkin-Elmer-2000 FT-IR spectrophotometer; ν in cm−1. For the 1H-, 13C- and 2D-NMR spectra, we used Varian-Mercury-500 and Varian-Unity-Plus-400 spectrometers; δ in ppm rel. to Me4Si, J in Hz. For the ESI and HRESIMS, we used a Bruker APEX-II mass spectrometer, in m/z.
Uv 240 spectrophotometer
Manufactured by Jasco
Sourced in Japan
The UV-240 spectrophotometer is a laboratory instrument used to measure the absorbance or transmittance of light in the ultraviolet and visible regions of the electromagnetic spectrum. It is designed to quantify the concentration of substances in solution by analyzing their absorption or transmission of specific wavelengths of light.
Automatically generated - may contain errors
Lab products found in correlation
Apex 2 mass spectrometer, by Bruker (10 mentions)
Dip 370 polarimeter, by Jasco (9 mentions)
Spherical c18 column, by Waters Corporation (8 mentions)
Spherical c18 100a reversed phase silica gel rp 18, by Silicycle (7 mentions)
Silica gel 60, by Merck Group (7 mentions)
2000 ft ir spectrophotometer, by PerkinElmer (6 mentions)
Precoated silica gel 60 f254 plates, by Merck Group (6 mentions)
Unity plus 400, by Agilent Technologies (5 mentions)
Mercury 500, by Agilent Technologies (4 mentions)
2000 ft ir spectrometer, by PerkinElmer (3 mentions)
Silica gel 60 f254, by Merck Group (3 mentions)
Vnmrs 600, by Agilent Technologies (2 mentions)
F254 precoated plates, by Merck Group (1 mentions)
Ftir spectrophotometer, by PerkinElmer (1 mentions)
Inova 500, by Agilent Technologies (1 mentions)
Mercury 400, by Agilent Technologies (1 mentions)
Fp 8200 spectrofluorimeter, by Jasco (1 mentions)
Ft ir 4100 spectrometer, by Jasco (1 mentions)
Aviii 500 spectrometer, by Bruker (1 mentions)
Inova 500 spectrometer, by Agilent Technologies (1 mentions)
13 protocols using uv 240 spectrophotometer
1
Analytical Techniques for Chemical Characterization
2
Physicochemical Characterization of Compound
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Melting point was determined using a Yanaco micro-melting point apparatus (Yanaco Co., Ltd., Kyoto, Japan). The infrared (IR) spectrum (KBr) was obtained using a 2000 FT-IR spectrometer (Perkin Elmer, Norwalk, CT, USA). The ultraviolet (UV) spectrum was measured with a UV-240 spectrophotometer (Jasco, Tokyo, Japan). The proton nuclear magnetic resonance (1H-NMR) spectra were acquired using a Varian Inova 500 (Varian Inc., Palo Alto, CA, USA) operating at a 500-MHz frequency. Electrospray ionization (ESI)-mass spectra were performed in a positive ion mode using an APEX II mass spectrometer (Bruker, Billerica, MA, USA). Silica gel 60 F-254 (Merck, Darmstadt, Germany) was used for preparative thin-layer chromatography (Prep TLC) and TLC. Column chromatography was followed out using Silica gel 60 (70–230, 230–400 mesh; Merck).
3
Analytical Methods for Natural Product Research
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For the TLC, we used silica gel 60 F254-precoated plates (Merck); for column chromatography (CC), we used silica gel 60 (70–230 or 230–400 mesh, Merck) and Spherical C18 100A Reversed Phase Silica Gel (RP-18) (particle size: 20–40 μm) (Silicycle). For the HPLC analysis, we used a spherical C18 column (250 × 10 mm, 5 μm) (Waters) and LDC-Analytical-III apparatus. For the UV spectra, we used a Jasco UV-240 spectrophotometer, with λmax (log ε) in nm. For optical rotation, we used a Jasco DIP-370 polarimeter, in CHCl3. For the IR spectra, we used a Perkin-Elmer-2000 FT-IR spectrophotometer, with ν in cm−1. For the 1H-, 13C-, and 2D-NMR spectra, we used Varian-VNMRS-600 and Varian-Unity-Plus-400 spectrometers; δ in ppm relative to Me4Si, J in Hz. For the ESI and HRESIMS, we used a Bruker APEX-II mass spectrometer, in m/z.
4
Column Chromatography Protocols for Structural Elucidation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Column chromatography (CC): silica gel 60 (70–230 or 230–400 mesh, Merck, Meguro City, Tokyo, Japan) and Reversed Phase Silica Gel (RP-18) (particle size: 20–40 μm) (Silicycle, Québec, QC, Canada). TLC: silica gel 60 F254 precoated plates (Merck) and Spherical C18 100A IR Spectra were measured on a Perkin-Elmer-2000 FT-IR spectrophotometer; 1H-, 13C- and 2D-NMR spectra were run on Varian-Mercury-500 using chloroform-d as the solvent; EI-MS: VG-Biotech Quatro-5022 mass spectrometer; m/z (rel. %). HR-EI-MS spectra were recorded on a Finnigan/Thermo Quest NAT mass spectrometer. UV spectra were run on a Jasco UV-240 spectrophotometer, λmax (log ε) in nm. Optical rotation: Jasco DIP-370 polarimeter; in CHCl3. HPLC: spherical C18 column (250 × 10 mm, 5 μm) (Waters, Milford, MA, USA) and LDC-Analytical-III apparatus.
5
Comprehensive Analytical Techniques for Compound Characterization
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
TLC: silica gel 60 F254 precoated plates (Merck, Darmstadt, Germany). Column chromatography (CC): silica gel 60 (70–230 or 230–400 mesh, Merck) and Spherical C18 100A Reversed Phase Silica Gel (RP-18) (particle size: 20–40 μm) (SiliCycle, Quebec City, Canada). HPLC: Spherical C18 column (250 mm × 10 mm, 5μm) (Waters, Milford, MA, USA); LDC-Analytical-III apparatus. UV Spectra: Jasco UV-240 spectrophotometer; λmax (log ε) in nm. Optical rotation: Jasco DIP-370 polarimeter; in CHCl3. IR Spectra: Perkin-Elmer-2000 FT-IR spectrophotometer; ν in cm−1. 1H-, 13C-, and 2D-NMR spectra: Varian-Mercury-500 and Varian-Unity-Plus-400 spectrometers; δ in ppm rel. to Me4Si, J in Hz. ESI and HRESIMS: Bruker APEX-II mass spectrometer; in m/z.
6
Analytical Techniques for Chemical Characterization
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
TLC: silica gel 60 F254 precoated plates (Merck). Column chromatography (CC): silica gel 60 (70–230 or 230–400 mesh, Merck) and Spherical C18 100A Reversed Phase Silica Gel (RP-18) (particle size: 20–40 μm) (Silicycle). HPLC: Spherical C18 column (250 × 10 mm, 5μm) (Waters); LDC-Analytical-III apparatus. UV Spectra: Jasco UV-240 spectrophotometer; λmax (log ε) in nm. Optical rotation: Jasco DIP-370 polarimeter; in CHCl3. IR Spectra: Perkin-Elmer-2000 FT-IR spectrophotometer; ν in cm−1. 1H-, 13C- and 2D-NMR spectra: Varian-Mercury-400 and Varian-Unity-Plus-400 spectrometers; δ in ppm relative to Me4Si, J in Hz. ESI and HRESIMS: Bruker APEX-II mass spectrometer; in m/z.
7
Detailed Chromatographic Techniques for Compound Separation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For TLC, we used silica gel 60 F254 precoated plates (Merck); for column chromatography (CC), silica gel 60 (70–230 or 230–400 mesh, Merck) and Spherical C18 100A Reversed Phase Silica Gel (RP-18) (particle size: 20–40 μm) (Silicycle). For HPLC, we used spherical C18 column (250 × 10 mm, 5 μm) (Waters) and LDC-Analytical-III apparatus. For the UV spectra, we used a Jasco UV-240 spectrophotometer, λmax (log ε) in nm. For the optical rotation, we used Jasco DIP-370 polarimeter, in CHCl3. For the IR spectra, we used a Perkin-Elmer-2000 FT-IR spectrophotometer; ν in cm−1. For the 1H-, 13C- and 2D-NMR spectra, we used Varian-Mercury-500 and Varian-Unity-Plus-400 spectrometers; δ in ppm rel. to Me4Si, J in Hz. For ESI and HRESIMS, we used a Bruker APEX-II mass spectrometer, in m/z.
8
Spectroscopic Characterization of Analytical Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All the chemicals and
reagents were of analytical grade and purchased from Sigma-Aldrich
and used without purification. A JASCO FT IR 4100 spectrometer was
used to record the absorption frequencies for the compounds. Bruker
Advance instruments (400 MHz for 1H and 100 MHz for 13C) were used to record the 1H and 13C NMR spectra using DMSO-d6 as solvent.
The reactions were monitored by using silica gel-precoated TLC F254
Merck plates. A Shimadzu UV-240 spectrophotometer and JASCO FP-8200
spectrofluorimeter were used to record absorption and fluorescence
spectra of the compounds using standard quartz cuvettes of 1 cm in
path length. The recorded excitation and emission slit width was 5.0
nm at 24 ± 1 °C
temperature. A PerkinElmer 2400 series II Elemental CHNS analyzer
was used to perform the elemental analysis. Mass spectra of the compounds
were obtained on an HR mass spectrometer.
reagents were of analytical grade and purchased from Sigma-Aldrich
and used without purification. A JASCO FT IR 4100 spectrometer was
used to record the absorption frequencies for the compounds. Bruker
Advance instruments (400 MHz for 1H and 100 MHz for 13C) were used to record the 1H and 13C NMR spectra using DMSO-d6 as solvent.
The reactions were monitored by using silica gel-precoated TLC F254
Merck plates. A Shimadzu UV-240 spectrophotometer and JASCO FP-8200
spectrofluorimeter were used to record absorption and fluorescence
spectra of the compounds using standard quartz cuvettes of 1 cm in
path length. The recorded excitation and emission slit width was 5.0
nm at 24 ± 1 °C
temperature. A PerkinElmer 2400 series II Elemental CHNS analyzer
was used to perform the elemental analysis. Mass spectra of the compounds
were obtained on an HR mass spectrometer.
9
Analytical Methods for Compound Characterization
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For the TLC, we used silica gel 60 F254-precoated plates (Merck); for column chromatography (CC), we used silica gel 60 (70–230 or 230–400 mesh, Merck) and Spherical C18 100A Reversed Phase Silica Gel (RP-18) (particle size: 20–40 μm) (Silicycle). For the HPLC analysis, we used a spherical C18 column (250 mm × 10 mm, 5 μm) (Waters) and LDC-Analytical-III apparatus. For the UV spectra, we used a Jasco UV-240 spectrophotometer, with λmax (log ε) in nm. For optical rotation, we used a Jasco DIP-370 polarimeter, in CHCl3. For the IR spectra, we used a Perkin-Elmer-2000 FT-IR spectrophotometer, with ν in cm−1. For the 1H-, 13C-, and 2D-NMR spectra, we used Varian-VNMRS-600 and Varian-Unity-Plus-400 spectrometers; δ in ppm relative to Me4Si, J in Hz. For the ESI and HRESIMS, we used a Bruker APEX-II mass spectrometer, in m/z.
10
Spectroscopic Characterization of Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Ultraviolet (UV) spectra were obtained on a Jasco UV-240 spectrophotometer. Infrared (IR) spectra (neat or KBr) were recorded on a Perkin Elmer 2000 FT-IR spectrometer.
Nuclear magnetic resonance (NMR) spectra, including correlation spectroscopy (COSY), rotating frame nuclear Overhauser effect spectroscopy (ROESY), nuclear Overhauser effect spectroscopy (NOESY), heteronuclear multiple-bond correlation (HMBC), and heteronuclear single-quantum coherence (HSQC) experiments, were acquired using a BRUKER AVIII-500 spectrometer (Bruker, Bremen, Germany), operating at 500 MHz (1H) and 125 MHz (13C), respectively, with chemical shifts given in the ppm (δ) using tetramethylsilane (TMS) as an internal standard. Electrospray ionization (ESI) and high-resolution electrospray ionization (HRESI)-mass spectra were recorded on a Bruker APEX II Mass Spectrometer (Bruker, Bremen, Germany). Silica gel [70–230 mesh (63–200 μm) and 230–400 mesh (40–63 μm), Merck] was used for column chromatography (CC). Silica gel 60 F-254 (Merck, Darmstadt, Germany) was used for thin-layer chromatography (TLC) and preparative thin-layer chromatography (PTLC).
Nuclear magnetic resonance (NMR) spectra, including correlation spectroscopy (COSY), rotating frame nuclear Overhauser effect spectroscopy (ROESY), nuclear Overhauser effect spectroscopy (NOESY), heteronuclear multiple-bond correlation (HMBC), and heteronuclear single-quantum coherence (HSQC) experiments, were acquired using a BRUKER AVIII-500 spectrometer (Bruker, Bremen, Germany), operating at 500 MHz (1H) and 125 MHz (13C), respectively, with chemical shifts given in the ppm (δ) using tetramethylsilane (TMS) as an internal standard. Electrospray ionization (ESI) and high-resolution electrospray ionization (HRESI)-mass spectra were recorded on a Bruker APEX II Mass Spectrometer (Bruker, Bremen, Germany). Silica gel [70–230 mesh (63–200 μm) and 230–400 mesh (40–63 μm), Merck] was used for column chromatography (CC). Silica gel 60 F-254 (Merck, Darmstadt, Germany) was used for thin-layer chromatography (TLC) and preparative thin-layer chromatography (PTLC).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!