Following dietary manipulation for 7 days, male flies were fasted for 22 hours (ZT9 until ZT7 next day) and then placed for 30 minutes on 1% agar containing non-caloric L-glucose (CarboSynth) at the concentrations indicated and colored with 0.5% blue dye (McCormick Culinary). Flies were kept at 25°C for the entirety of the experiment.
L glucose
Manufactured by Biosynth
Sourced in Switzerland
L-glucose is a rare sugar molecule that is the enantiomer of the more common D-glucose. It serves as a useful tool in biochemical research and experiments, providing a means to study the specificity of glucose-binding proteins and enzymes.
Automatically generated - may contain errors
Lab products found in correlation
Penicillin streptomycin, by Carl Roth (1 mentions)
L glutamine, by Biosynth (1 mentions)
Mercaptoethanol, by Carl Roth (1 mentions)
Lysogeny broth medium, by Carl Roth (1 mentions)
Isopropyl β d thiogalactopyranoside iptg, by Carl Roth (1 mentions)
Hepes, by Thermo Fisher Scientific (1 mentions)
L glutamine, by Thermo Fisher Scientific (1 mentions)
Ampicillin, by Carl Roth (1 mentions)
Pancoll, by PAN Biotech (1 mentions)
Phosphate buffered saline (pbs), by PAN Biotech (1 mentions)
Dimethyl sulfoxide (dmso), by Carl Roth (1 mentions)
Dulbecco s modified eagle s medium dmem, by Capricorn (1 mentions)
4 nitrobenzaldehyde, by Merck Group (1 mentions)
D fructose, by Biosynth (1 mentions)
D ribose, by Merck Group (1 mentions)
L idose, by Biosynth (1 mentions)
D glucose, by Merck Group (1 mentions)
Nadph, by Merck Group (1 mentions)
3 protocols using l glucose
1
Fly Fasting and Glucose Feeding
2
Antibody Staining and Cell Culture Protocol
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The following antibodies were purchased from BioLegend (San Diego, CA, USA): Alexa FluorTM 647 αHisTag Antibody (Cat. No.: 652513) and Purified anti-HisTag Antibody (Cat. No.: 652501). Peroxidase AffiniPure Donkey αMouse IgG (H + L) antibody (Cat. No.: 715-035-151) was bought from Jackson ImmunoResearch Laboratories, Inc. (West Grove, PA, USA).
Roswell Park Memorial Institute 1640 medium (RPMI 1640), fetal bovine serum (FBS), L-Glutamine, HEPES, and 0.05% Trypsin-EDTA (1×) were commercially bought from Thermo Fisher Scientific (Waltham, MA, USA). DMSO, Isopropyl-β-D-thiogalactopyranoside (IPTG), penicillin/streptomycin, ß-mercaptoethanol, ampicillin, and lysogeny broth (LB)-medium were purchased from Carl Roth GmbH (Karlsruhe, Germany). PBS, Pancoll, and Minimum Essential Medium (MEM) Non-Essential Amino Acid Solution (100×) were obtained from PAN Biotech (Aidenbach, Germany). Dulbecco´s Modified Eagle´s Medium (DMEM) was provided by Capricorn Scientific GmbH (Ebsdorfergrund, Germany). The DMEM was already supplied with 1.0 g/L L-glucose, 1.0 g/L L-Glutamine, 1.0 g/L sodium pyruvate, and 3.7 g/L NaHCO3 D-Luciferin Firefly was obtained from Biosynth (Staad, Switzerland).
Roswell Park Memorial Institute 1640 medium (RPMI 1640), fetal bovine serum (FBS), L-Glutamine, HEPES, and 0.05% Trypsin-EDTA (1×) were commercially bought from Thermo Fisher Scientific (Waltham, MA, USA). DMSO, Isopropyl-β-D-thiogalactopyranoside (IPTG), penicillin/streptomycin, ß-mercaptoethanol, ampicillin, and lysogeny broth (LB)-medium were purchased from Carl Roth GmbH (Karlsruhe, Germany). PBS, Pancoll, and Minimum Essential Medium (MEM) Non-Essential Amino Acid Solution (100×) were obtained from PAN Biotech (Aidenbach, Germany). Dulbecco´s Modified Eagle´s Medium (DMEM) was provided by Capricorn Scientific GmbH (Ebsdorfergrund, Germany). The DMEM was already supplied with 1.0 g/L L-glucose, 1.0 g/L L-Glutamine, 1.0 g/L sodium pyruvate, and 3.7 g/L NaHCO3 D-Luciferin Firefly was obtained from Biosynth (Staad, Switzerland).
3
Enzymatic Synthesis of Rare Sugars
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L-glucose, L-idose, L-sorbose and L-threose were from Carbosynth. D-fructose, D-glucose, D,Lglyceraldehyde (GAL), NADPH, 4-nitrobenzaldehyde and D-ribose were from Sigma Aldrich. DTT was from Inalco. HNE was prepared as described [37] . All other chemicals were of reagent grade.
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