Tissue culture grade dmso

Manufactured by Merck Group

Tissue culture grade DMSO is a highly purified form of dimethyl sulfoxide (DMSO) that is specifically designed for use in cell and tissue culture applications. It serves as a cryoprotectant, helping to preserve the viability and integrity of cells during freezing and thawing processes.

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5 protocols using tissue culture grade dmso

Synthesis and Storage of SBI-183

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SBI-183 (molecular weight 296.3723 g/mol) was purchased from ChemBridge Corp. (San Diego CA). Compounds were dissolved in tissue culture-grade DMSO (Sigma-Aldrich) and kept at −80°C as 100 mM stock solutions. See Supplementary Figure 1 for the chemical structure of SBI-183.

Fifield A.L., Hanavan P.D., Faigel D.O., Sergienko E., Bobkov A., Meurice N., Petit J.L., Polito A., Caulfield T.R., Castle E.P., Copland J.A., Mukhopadhyay D., Pal K., Dutta S.K., Luo H., Ho T.H, & Lake D.F. (2019). Molecular Inhibitor of QSOX1 Suppresses Tumor Growth in vivo. Molecular cancer therapeutics, 19(1), 112-122.

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Synthesis and Characterization of Fluorescent Nanoparticles

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PMAn,
CDDP, anhydrous DMF,
silver nitrate, o-phenylenediamine, N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide
hydrochloride (EDC), N,N-dimethyl
amino pyridine (DMAP), pyrene, ethylenediamine, rhodamine B isothiocyanate,
dimethyl sulfoxide (DMSO-d₆), methanol-d₄, and silicon wafer for FESEM were bought from
Sigma-Aldrich. PTX was purchased from Selleck Chemical. Dialysis membranes
(3.5 kDa) were purchased from Spectrum Labs. MCF7 cells were procured
from ECACC. Dulbecco’s modified Eagle’s medium (DMEM),
fetal bovine serum (FBS), LysoTracker Green DND-26, Hoechst 33342,
SlowFade Gold antifade, and Alexa Fluor-conjugated phalloidin 568
were purchased from Invitrogen. MTT reagent and tissue culture grade
DMSO were purchased from Sigma-Aldrich. 96-well flat-bottomed tissue-culture
plates were obtained from Corning.

Palvai S., Anandi L., Sarkar S., Augustus M., Roy S., Lahiri M, & Basu S. (2017). Drug-Triggered Self-Assembly of Linear Polymer into Nanoparticles for Simultaneous Delivery of Hydrophobic and Hydrophilic Drugs in Breast Cancer Cells. ACS Omega, 2(12), 8730-8740.

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TCDD and SHP-1 Inhibitor Protocol

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99.1% pure TCDD dissolved in dimethyl sulfoxide (DMSO) was purchased from AccuStandard Inc., (New Haven, CT). Tissue culture grade DMSO was purchased from Sigma Aldrich (St. Louis, MO). Sodium Stibogluconate (SSG) (EMD Millipore, Billerica, MA) also known as sodium antimony gluconate is a potent inhibitor of SHP-1 phosphatase (Pathak and Yi, 2001 ) and was used at a final concentration of 10µg/ml in vitro. SSG was dissolved in water at 70°C and was freshly prepared for all experiments involving the inhibitor.

Phadnis-Moghe A.S., Li J., Crawford R.B, & Kaminski N.E. (2016). SHP-1 is Directly Activated by the Aryl Hydrocarbon Receptor and regulates BCL-6 in the presence of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Toxicology and applied pharmacology, 310, 41-50.

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TCDD-Induced SHP-1 Phosphatase Inhibition

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99.1% pure TCDD dissolved in dimethyl sulfoxide (DMSO) was purchased from AccuStandard Inc., (New Haven, CT). Tissue culture grade DMSO was purchased from Sigma Aldrich (St. Louis, MO) and was used to dilute TCDD and used as a vehicle control. Sodium Stibogluconate (SSG) (EMD Millipore, Billerica, MA) also known as sodium antimony gluconate is a potent inhibitor of SHP-1 phosphatase [31 (link)] and was used at a final concentration of 10μg/ml in vitro. SSG was dissolved in water at 70°C and was freshly prepared for all experiments involving the inhibitor.

Blevins L.K., Zhou J., Crawford R, & Kaminski N.E. (2019). TCDD-mediated suppression of naïve human B cell IgM secretion involves aryl hydrocarbon receptor-mediated reduction in STAT3 serine 727 phosphorylation and is restored by Interferon-γ. Cellular signalling, 65, 109447.

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TCDD and AHR Antagonist Preparation

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TCDD (99.1% purity) dissolved in dimethyl sulfoxide (DMSO) was purchased from AccuStandard Inc., (New Haven, CT). Tissue culture grade DMSO was purchased from Sigma Aldrich (St. Louis, MO) and was used to dilute TCDD and also as a vehicle control. AHR antagonist, CH223191 (≥98.0% purity), dissolved in DMSO was purchased from Tocris (Bristol, United Kingdom).

Blevins L.K., Zhou J., Crawford R.B, & Kaminski N.E. (2021). Identification of a Sensitive Human Immunological Target of Aryl Hydrocarbon Receptor Activation: CD5+ Innate-Like B Cells. Frontiers in Immunology, 12, 635748.

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