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Phosphor s6

Manufactured by Cell Signaling Technology
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Phosphor-S6 is a laboratory product that detects phosphorylated ribosomal protein S6. It is used to assess the activation of the mTOR signaling pathway.

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Lab products found in correlation

Phospho alk y1282 1283, by Cell Signaling Technology (1 mentions) Gapdh, by Merck Group (1 mentions) Enhanced chemiluminescence detection, by Thermo Fisher Scientific (1 mentions) Ab104835, by Abcam (1 mentions) Ab652, by Abcam (1 mentions) Phosphor akt, by Cell Signaling Technology (1 mentions) Phosphor mtor, by Cell Signaling Technology (1 mentions) Hif 1α, by Cayman Chemical (1 mentions) Anti rabbit igg hrp conjugate, by Promega (1 mentions) Cpt1a, by Proteintech (1 mentions) Phosphor p70s6k, by Cell Signaling Technology (1 mentions) Odyssey infrared imaging system, by LI COR (1 mentions) Doxorubicin, by Merck Group (1 mentions) Pdcd4, by Cell Signaling Technology (1 mentions) Phospho s6k, by Cell Signaling Technology (1 mentions) Torin2, by Selleck Chemicals (1 mentions) Rad001, by Selleck Chemicals (1 mentions) Anti gfp antibody, by Abcam (1 mentions) Rapamycin, by Selleck Chemicals (1 mentions) Mln0128, by Selleck Chemicals (1 mentions)

4 protocols using phosphor s6

1

Protein Signaling Pathway Analysis

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5×105 cells were treated in 6 well plates for 6 hours with the indicated drugs. Cells protein lysates were prepared as previously described (6 , 11 (link)). Phospho-ERK (T202/Y204), ERK, S6, phosphor S6, phospho-AKT (S473 and T308), AKT, phospho-ALK (Y1282/1283), and ALK antibodies were obtained from Cell Signaling Technology. GAPDH was purchased from Millipore.
Friboulet L., Li N., Katayama R., Lee C.C., Gainor J.F., Crystal A.S., Michellys P.Y., Awad M.M., Yanagitani N., Kim S., Pferdekamper A.C., Li J., Kasibhatla S., Sun F., Sun X., Hua S., McNamara P., Mahmood S., Lockerman E.L., Fujita N., Nishio M., Harris J.L., Shaw A.T, & Engelman J.A. (2014). The ALK inhibitor ceritinib overcomes crizotinib resistance in non-small cell lung cancer. Cancer discovery, 4(6), 662-673.
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2

Western Blotting Protein Detection

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Immunoblotting was performed as described previously6 (link). Primary antibodies were followed by mouse- or rabbit-conjugated horseradish peroxidase (HRP). HRP-conjugated antibodies (anti-mouse or anti-rabbit IgG HRP conjugate, Promega) were detected by enhanced chemiluminescence detection (Thermofisher). This included the following antibodies: Akt (9272, Cell Signaling), Cpt1a (15184-1-AP, Proteintech group), Hif-1α (10006421, Cayman Chemical Company), phosphor-Akt (4060, Cell Signaling), PIK3cγ (ab140310, abcam), phosphor-mTOR (5536, Cell Signaling), PTEN (9552, Cell Signaling), phosphor-p70 S6K (9204, Cell Signaling). Alternatively, primary antibodies were followed by fluorescently labeled anti-mouse or rabbit antibodies (LiCor) and imaged using the Odyssey infrared imaging system (LiCor). This included the following antibodies: Glut1 (ab652, Abcam), hexokinase 2 (2867, Cell Signaling), hexokinase 1 (ab104835, Abcam), cytochrome C (556432, BD Biosciences), β-actin (A5441, Sigma) and phosphor-S6 (4858, Cell Signaling). All primary antibodies were used at a 1:1,000 dilution, with secondary HRP antibodies used at 1:8000. Secondary LiCor antibodies were used at 1:10000 (anti-mouse) or 1:8000 (anti-rabbit).
Gerriets V.A., Kishton R.J., Johnson M.O., Cohen S., Siska P.J., Nichols A.G., Warmoes M.O., de Cubas A.A., MacIver N.J., Locasale J.W., Turka L.A., Wells A.D, & Rathmell J.C. (2016). Foxp3 and Toll-like receptor signaling balance Treg cell anabolic metabolism for suppression. Nature immunology, 17(12), 1459-1466.
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3

Phosphorylation Signaling Pathway Analysis

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Anti-mouse S6K, S6, 4EBP, PDCD4, beta-Actin, phospho-S6K, phosphor-S6, phosphor-4EBP, and phospho-4EBP-Alexa647 antibodies were purchased from Cell Signaling Technologies (Danvers, MA, USA). Anti-mouse phoshoS6-PE, anti-mouse CD45.1 Pacific Blue, and anti-mouse Ki67 Brilliant Violet 605 antibodies were purchased from Biolegend (San Diego, CA, USA). Anti-GFP antibody purchased from Abcam (Cambridge, UK). Cytarabine arabinoside (Ara-C) and doxorubicin were purchased form Sigma-Aldrich (St. Louis, MO, USA). Rapamycin, Rad001, Torin2, AZD0855, and MLN(0128) were purchased from Selleckchem (Houston, TX, USA).
Oki T., Mercier F., Kato H., Jung Y., McDonald T.O., Spencer J.A., Mazzola M.C., van Gastel N., Lin C.P., Michor F., Kitamura T, & Scadden D.T. (2021). Imaging dynamic mTORC1 pathway activity in vivo reveals marked shifts that support time-specific inhibitor therapy in AML. Nature Communications, 12, 245.
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4

Analyzing Signaling Pathways in Cellular Processes

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Antibodies against PKM2 (4053, 1:1000), Jak2 (3230, 1:1000), phosphor‐Jak2 (8082, 1:500), Stat3 (9132, 1:1000), phosphor‐Stat3 (9145, 1:1000), TGF‐β (3711, 1:1000), Smad2/3 (8685, 1:1000), phosphor‐Smad2/3 (8828, 1:1000), S6 (2217s, 1:1000), phosphor‐S6 (2211s, 1:5000) and GAPDH (2118,1:10000) were from Cell Signaling Technology (Danvers, MA, USA). Rapamycin was from MedChem Express (HY‐10219, New Jersey, USA). Shikonin was from Selleck (S8279, Houston, TX, USA). N‐acetyl‐L‐cysteine (NAC) was from Beyotime Institute of Biotechnology (S0077, Shanghai, China).
Zhang X., Zheng C., Gao Z., Wang L., Chen C., Zheng Y, & Meng Y. (2021). PKM2 promotes angiotensin‐II‐induced cardiac remodelling by activating TGF‐β/Smad2/3 and Jak2/Stat3 pathways through oxidative stress. Journal of Cellular and Molecular Medicine, 25(22), 10711-10723.
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