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The GR66a-Gal4 is a genetic tool used to study gustatory receptor neurons in Drosophila. It is a driver line that expresses the Gal4 transcription factor under the control of the GR66a promoter, which is specifically active in a subset of gustatory receptor neurons.

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Lab products found in correlation

Gr32a gal4, by Bloomington Drosophila Stock Center (1 mentions) Gr43a gal4, by Bloomington Drosophila Stock Center (1 mentions) Orco gal4, by Bloomington Drosophila Stock Center (1 mentions) Gr33agal4, by Bloomington Drosophila Stock Center (1 mentions) Uas dtrpa1, by Bloomington Drosophila Stock Center (1 mentions) 13xlexaop2 ivs gcamp6s p10, by Bloomington Drosophila Stock Center (1 mentions) Uas rpr, by Bloomington Drosophila Stock Center (1 mentions) Uas campari, by Bloomington Drosophila Stock Center (1 mentions)

2 protocols using gr66a gal4

1

Genetic Tools for Neuromodulation in Drosophila

Cited 2 times
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Wild type (OrgR) crossed to UAS-dTrpA1 (Bloomington #26263) served as control in Figs 1, 2, 3 and 5. Hugin-Gal4 (HugS3-Gal4 (ref. 13 (link)), Bloomington# 58769), GR66a-Gal4 (second Chr., gift from K.Scott31 (link)(formerly described as GR66C1)), GR66a-Gal4 (third Chr., Bloomington# 57670 used in Fig. 7b), Hugin-lexA (Hug1.2-lexA attp40 (ref. 32 (link))), HugPC-Gal4 (see generation of this Gal4-line below), UAS-eNpHR-YFP (Bloomington# 41753, referred here as UAS-YFP in Supplementary Fig. 3, since this homozygous line together with HugPC-Gal4 was used as fluorescent marker only), UAS-CaMPARI (Bloomington #58761), UAS-rpr;;UAS-hid (UAS-rpr (Bloomington# 5823) crossed homozygous to UAS-hid33 (link)), UAS-shibireTS34 (link), lexAop-CD4::spGFP11 and UAS-CD4::spGFP1-10 were gifts from K. Scott19 (link), 13x LexAop2-IVS-GCamP6s-p10 (Bloomington #44274).
Hückesfeld S., Peters M, & Pankratz M.J. (2016). Central relay of bitter taste to the protocerebrum by peptidergic interneurons in the Drosophila brain. Nature Communications, 7, 12796.
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2

Comprehensive Gustatory Receptor Neuron Profiling

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The following GAL4 driver and UAS effector lines were used: Gr2a-GAL4 (Bloomington #57589), Gr10a-GAL4 (Bloomington #57597), Gr22b-GAL4 (Bloomington #57604), Gr22e-GAL4 (Bloomington #57608), Gr23a-GAL4 (Bloomington #57611), Gr28a-GAL4 (Bloomington #57614 and #57613), Gr28b-GAL4 (Scott et al., 2001 (link)), Gr28b.a-GAL4 (Bloomington #57615), Gr28b.e-GAL4 (Bloomington #57621), Gr32a-GAL4 (Bloomington #57622), Gr33a-GAL4 (Bloomington #57624), Gr39a.a-GAL4 (Bloomington #57631), Gr39a.b-GAL4 (Bloomington #57632), Gr39a.d-GAL4 (Bloomington #57634), Gr39b-GAL4 (Bloomington #57635), Gr43a-GAL4 (Bloomington #57636 and #57637), Gr43aGAL4 knock-in (Miyamoto et al., 2012 (link)), Gr57a-GAL4 (Bloomington #57642), Gr58b-GAL4 (Bloomington #57646), Gr59a-GAL4 (Bloomington #57648), Gr59d-GAL4 (Bloomington #57652), Gr63a-GAL4 (Bloomington #57660), Gr66a-GAL4 (Scott et al., 2001 (link)), Gr68a-GAL4 (Bloomington #57671), Gr77a-GAL4 (Bloomington #57672), Gr93a-GAL4 (Bloomington #57679), Gr93b-GAL4 (Bloomington #57680), Gr93c-GAL4 (Bloomington #57681), Gr93d-GAL4 (Bloomington #57684), 94a-GAL4 (Bloomington #57686), Orco-GAL4 (Bloomington #23909) and 10X-UAS-mCD8::GFP (Bloomington #32184)
Miroschnikow A., Schlegel P., Schoofs A., Hueckesfeld S., Li F., Schneider-Mizell C.M., Fetter R.D., Truman J.W., Cardona A, & Pankratz M.J. (2018). Convergence of monosynaptic and polysynaptic sensory paths onto common motor outputs in a Drosophila feeding connectome. eLife, 7, e40247.
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