Gallium, aminopropyltrimethoxysilane (APTMS), and carbonylated β-cyclodextrin (β-CD) were purchased from Aladdin. Park Memorial Institute 1640 (RPMI 1640) medium, 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindo-dicarbocyanine perchlorate (DiD), dimethyl sulphoxide (DMSO), fetal bovine serum (FBS), 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide solution (MTT), penicillin streptomycin, and 0.25% Trypsin-EDTA were obtained from Life Technologies Corporation; ceramic transducers (H4P101000, ϕ10∗2 mm) were purchased from Fukeda.
1 1 dioctadecyl 3 3 3 3 tetramethylindodicarbocyanine perchlorate
Manufactured by Thermo Fisher Scientific
Sourced in United States
1,1′-dioctadecyl-3,3,3′,3′-tetramethylindodicarbocyanine perchlorate is a fluorescent dye used for labeling lipophilic structures. It has an absorption maximum at 644 nm and an emission maximum at 665 nm.
Automatically generated - may contain errors
Lab products found in correlation
Trypsin edta, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Nocodazole, by Merck Group (1 mentions)
Cytochalasin d, by Merck Group (1 mentions)
Dicyclohexylcarbodiimide, by Thermo Fisher Scientific (1 mentions)
N hydroxysuccinimide, by Thermo Fisher Scientific (1 mentions)
Doxorubicin, by LC Laboratories (1 mentions)
Paclitaxel, by AK Scientific (1 mentions)
Genios pro, by Tecan (1 mentions)
1 1 dioctadecyl 3 3 3 3 tetramethylindocarbocyanine perchlorate dii, by Thermo Fisher Scientific (1 mentions)
Cholesterol chol, by Fujifilm (1 mentions)
Hoechst 33342, by AnaSpec (1 mentions)
Drabkin s reagent, by Merck Group (1 mentions)
Sulforhodamine b, by Thermo Fisher Scientific (1 mentions)
1 1 dioctadecyl 3 3 3 3 tetramethylindocarbocyanine perchlorate, by Thermo Fisher Scientific (1 mentions)
Collagenase type 1, by Merck Group (1 mentions)
Aprotinin, by Merck Group (1 mentions)
7 protocols using 1 1 dioctadecyl 3 3 3 3 tetramethylindodicarbocyanine perchlorate
1
Gallium-based Nanoparticle Synthesis
2
Fluorescent Dyes and Inhibitors Protocol
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1, 1′-dioctadecyl-3, 3, 3′, 3′-tetramethylindodicarbocyanine perchlorate (Did) and 3, 3′-dioctadecyloxacarbocyanine perchlorate (Diol) were obtained from Life Technologies. Oseltamivir carboxylate was purchased from Sequoia Research Products Ltd. Cytochalasin D and Nocodazole were purchased from Sigma Aldrich.
3
Synthesis and Characterization of Dye-Labeled Compounds
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Paclitaxel (98%) was purchased
from AK Scientific Inc. (CA, USA). Doxorubicin (>99%) was purchased
from LC Laboratories (MA, USA). 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindodicarbocyanine
perchlorate (DiD) was purchased from Invitrogen (NY, USA). N-hydroxysuccinimide (NHS) and dicyclohexylcarbodiimide
(DCC) were purchased from Alfa Aesar (MA, USA). 4-(Dimethylamino)
pridine (DMAP) was purchased from Calbiochem–Novabiochem Corporation
(CA, USA). FTS was synthesized and purified following a published
literature.33 (link)
from AK Scientific Inc. (CA, USA). Doxorubicin (>99%) was purchased
from LC Laboratories (MA, USA). 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindodicarbocyanine
perchlorate (DiD) was purchased from Invitrogen (NY, USA). N-hydroxysuccinimide (NHS) and dicyclohexylcarbodiimide
(DCC) were purchased from Alfa Aesar (MA, USA). 4-(Dimethylamino)
pridine (DMAP) was purchased from Calbiochem–Novabiochem Corporation
(CA, USA). FTS was synthesized and purified following a published
literature.33 (link)
4
Labeling and Quantifying VLDL Particles
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Human VLDL (δ < 1.006 g/ml) was isolated from plasma by buoyant density ultracentrifugation and quantified by BCA protein assay (Pierce) (30 (link)). To label the particles, 1 to 2 mg of VLDL were combined with 100 μl of 3 mg/ml 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindodicarbocyanine perchlorate (Invitrogen) in DMSO and then re-isolated by ultracentrifugation. After incubation with VLDL for 30 min at 4 ºC, cells were rinsed with PBS and lysed by adding 0.1 M NaOH plus 0.1% SDS for 40 min at room temperature (30 (link)). Fluorescence intensity was measured with appropriate excitation and emission filters in a plate reader (TECAN GENios Pro) and normalized to total cell protein.
5
Lipid-Based Nanoparticle Formulation Protocol
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Hydrogenated soy phosphatidylcholine (HSPC) and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-n-[methoxy(polyethylene glycol)-2000] (mPEG2000-DSPE) were generously donated by NOF (Tokyo, Japan). Cholesterol (CHOL) was purchased from Wako Pure Chemical (Osaka, Japan). S-1, an oral fluorouracil antitumor drug that contains three pharmacological agents (tegafur, 5-chloro-2, 4-dihydroxypyridine, and potassium oxonate) was generously donated by Taiho Pharmaceutical (Tokyo, Japan). 1,1′-Dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate (DiI) and 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindodicarbocyanine perchlorate (DiD) were purchased from Invitrogen (Paisley, UK). Fluorescein isothiocyanate (FITC)-labeled rabbit anti-rat CD31 IgG heavy and light chain polyclonal antibody was purchased from Abcam (Cambridge, UK). Hoechst 33342 and 3,3′- diheptyloxacarbocyanine iodide (DiOC7) were purchased from AnaSpec (Freemont, CA, USA). FITC-dextran (M.W. 150,000) and Drabkin’s reagent was purchased from Sigma-Aldrich (St Louis, MO, USA). In Situ Cell Death Detection Kit (TMR red) was purchased from Roche Diagnostics (Indianapolis, IN, USA). All other reagents were of analytical grade.
6
Lipid Composition and Preparation
7
Comprehensive Protocol for Cell Tracking and Drug Screening
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Type I collagenase, trans-4-(aminomethyl)cyclohexanecarboxylic acid (AMCHA), thrombin, factor XIII, aprotinin, calcium chloride (CaCl2), epsilon-aminocaproic acid (EACA), 4-aminomethylbenzoic acid (PAMBA), and dimethyl sulfoxide (DMSO) were purchased from Sigma-Aldrich (Saint Louis, MO, USA). Fluorescent cell trackers such as 3,3′-dioctadecyloxacarbocyanine perchlorate (DiO, excitation, 488 nm; emission 525/50 nm), 1,1′-Dioctadecyl-3,3,3′,3′-tetramethylindodicarbocyanine perchlorate (DiD, excitation, 635 nm; emission, 655–730 nm), and DiIC18(7) (1,1′-dioctadecyl-3,3,3′,3′-tetramethylindotricarbocyanine iodide) (DiR, excitation, 750 nm; emission, 780 nm) were purchased from Invitrogen (Carlsbad, CA, USA). Drug treatments for cell line studies including methotrexate (MTX), paclitaxel (PTX), capecitabine (CAP), cyclophosphamide monohydrate (CYCLO), carboplatin (CARBO), epirubicin hydrochloride (EPI), and docetaxel (DTX) were purchased from MedKoo (Morrisville, NC, USA). Drug treatment of patient material using Anastrazole (Arimidex) was purchased from Selleck Chemicals (Houston, TX, USA).
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