Thp 1

Manufactured by Shanghai Cell Bank

Sourced in China, United States

The THP-1 is a human monocytic leukemia cell line. It is a suspension cell line derived from the peripheral blood of a 1-year-old male with acute monocytic leukemia. The THP-1 cell line is commonly used in research for the study of monocyte and macrophage biology.

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THP-1 cells (4 citations) THP-1 monocytes (2 citations)

15 protocols using thp 1

Immune Modulation in Colon Cancer Cells

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Colon cancer cell line HCT-116, HT-29, Caco-2, and Human monocytic leukemia cell line THP-1 (purchased from Shanghai Cell Bank, Chinese Academy of Sciences) were cultured in RPMI-1640 medium containing 10% fetal bovine serum (FBS) in an atmosphere containing 5% CO2 at 37 °C.
To generate THP-1 macrophages, THP-1 cells were treated with 100 ng/ml PMA for 48 h. Condition mediums (CMs) were collected from colon cancer cells and THP-1 macrophages after 96 h. THP-1 cells were treated with the CMs of colon cancer cells (30%) supplemented with PMA for 48 h. Macrophages-derived CM was used to stimulate HCT-116 cells for 48 h.

Zhao P., Wang B., Zhang Z., Zhang W, & Liu Y. (2019). Response gene to complement 32 expression in macrophages augments paracrine stimulation-mediated colon cancer progression. Cell Death & Disease, 10(10), 776.

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Modulating 5-FU Cytotoxicity in Cancer Cells

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Human mononuclear macrophage (THP-1) cells and human colorectal adenocarcinoma (Caco-2) cells were purchased from Shanghai Cell Bank. THP-1 cells were cultured in RPMI 1640 medium containing 10% fetal bovine serum, and Caco-2 cells were cultured in DMEM medium containing 10% fetal bovine serum. Both of the cells were used in this study after stable passage to the sixth generation in the environment of 37 °C and 5% CO₂. The cells in the logarithmic growth phase were inoculated in a 6-well plate with 1 × 10⁶ cells in each well. The normal control group (NC), 5-FU group (5-FU), 5-FU + sodium acetate group (NaAc), 5-FU + sodium propionate group (NaPc) and 5-FU + sodium butyrate group (NaB) were set up in the experiment. The cells in the NC group were not do any treatment. The NaAc group, NaPc group and NaB group cells were pretreated with 100 μmol/L NaAc, NaPc, NaB for 24 h, respectively. The cells in the 5-FU group, NaAc group, NaPc group and NaB group were treated with 5-FU for 24 h. The concentration of 5-FU was 2.5 mmol/L in THP-1 cells and 5 mmol/L in Caco-2 cells. All methods were carried out in accordance with relevant guidelines and regulations.

Yue X., Wen S., Long-kun D., Man Y., Chang S., Min Z., Shuang-yu L., Xin Q., Jie M, & Liang W. (2022). Three important short-chain fatty acids (SCFAs) attenuate the inflammatory response induced by 5-FU and maintain the integrity of intestinal mucosal tight junction. BMC Immunology, 23, 19.

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Cultivation and Isolation of AML Cell Lines

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MV4-11 cell line was kindly endowed by Professor R. Bhatia (City of Hope National Medical Center, Duarte, CA, USA). THP-1, HL-60, U937 and OCI-AML3 cell lines were purchased from the Shanghai Cell Bank of the Chinese Academy of Sciences. Kasumi-1 cell line was gifted by Professor Chen Saijuan (Shanghai Institute of Hematology, Shanghai, China). MV4-11 cells were maintained in Iscove’s Modified Dulbecco’s medium (IMDM, Gibco, Billings, MT, USA) supplemented with 10% fetal bovine serum (FBS, Gibco), and THP-1, HL-60, U937 and OCI-AML3 cells were cultured in Roswell Park Memorial Institute 1640 (RPMI 1640, Gibco) medium supplemented with 10% FBS at 37 °C in a humidified incubator containing 5% CO₂.
Bone marrow samples were obtained from AML patients following written informed consent. Mononuclear cells were isolated by Ficoll-Hypaque (Sigma-Aldrich, St. Louis, MO, USA) density gradient centrifugation. The study protocol was approved by the Ethics Committee of the First Affiliated Hospital of Zhejiang University, China.

Wei W., Huang S., Ling Q., Mao S., Qian Y., Ye W., Li F., Pan J., Lin X., Huang J., Huang X., Zhai Y., Sun J, & Jin J. (2022). Homoharringtonine is synergistically lethal with BCL-2 inhibitor APG-2575 in acute myeloid leukemia. Journal of Translational Medicine, 20, 299.

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Cultivation of Hematological Cancer Cell Lines

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293T/17, Namalwa (human Burkitt lymphoma cell), Karpas-299 (human anaplastic large cell lymphoma cells), Daudi (human Burkitt lymphoma cells), HL-60 (human myeloid leukemia cells), Su-DHL-4 (human diffuse large B-cell lymphoma cells), Kasumi-1 (human acute myeloid leukemia cells), HEL (human erythroleukemia leukemia cells), K562 (human chronic myeloid leukemia cells), THP1 (human monocyte leukemia cells) and Jurkat (human T lymphocytic leukemia cells) cell lines were purchased from the Shanghai Cell Bank, Chinese Academy of Sciences (Shanghai, China). The lentivirus packaging cell line 293T/17 was maintained in Dulbecco's modified Eagle's medium (DMEM; Hyclone; GE Healthcare Life Sciences, Logan, UT, USA). The remaining hematological tumor cells were cultured in Iscove's modified Dulbecco's medium (IMDM) or RPMI-1640 medium (Hyclone; GE Healthcare Life Sciences). Cell cultures were supplemented with 0.1 U/ml streptomycin, 0.1 U/µl penicillin and 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA). The cells were cultured at 37°C in a humidified atmosphere of 5% CO₂.

Yang M., Tian X., Fan Z., Yu W., Li Z., Zhou J., Zhang W, & Liang A. (2019). Targeting RAD51 enhances chemosensitivity of adult T-cell leukemia-lymphoma cells by reducing DNA double-strand break repair. Oncology Reports, 42(6), 2426-2434.

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Establishment of AML Cell Lines

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MG132 were purchased from Selleck Chemicals(Houston, Texas, USA). ST1326 and ABT199 was purchased from Sigma-Aldrich (St Louis, MO, USA) Cell culture HL-60, THP-1, OCI-AML2 and OCI-AML3 cell lines were purchased from Shanghai Cell Bank of the Chinese Academy of Sciences. KASUMI-1 cell line was gifted by Professor Chen Saijuan (Shanghai Institute of Hematology, Shanghai, China). These cells were cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum(FBS)(Gibco) at 37 °C in a humidi ed incubator containing 5% CO2. MV4-11 and MOLM-13 cell lines were a kind gift from Professor Ravi Bhatia (City of Hope National Medical Center, Duarte, CA, USA). These two cell lines were cultured in Iscove's Modi ed Dulbecco's Medium (IMDM) supplemented with 10% (FBS).
Diagnostic AML patient samples were puri ed by standard Ficoll-Hypaque (Sigma-Aldrich) density centrifugation, then cultured in RPMI 1640 with 10% FBS.

Mao S., Ling Q., Pan J., Li F., Huang S., Ye W., Wei W., Lin X., Yu Q., Wang Y., Huang X., Huang J., Wang J., & Jin J. (2021). Inhibition of CPT1a as a Prognostic Marker can Synergistically Enhance the Antileukemic Activity of ABT199.

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Culturing Diverse Acute Leukemia Cell Lines

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HL-60 (acute promyelocytic leukemia cell line), THP-1 (acute monocytic leukemia cell line), OCI-AML2 (acute myelomonocytic leukemia cell line) and OCI-AML3 (acute myelomonocytic leukemia cell line) cell lines were purchased from Shanghai Cell Bank of the Chinese Academy of Sciences. KASUMI-1 (acute myeloblastic leukemia cell line) cell line was gifted by Professor Chen Saijuan (Shanghai Institute of Hematology, Shanghai, China). These cells were cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum (FBS) (Gibco) at 37 °C in a humidified incubator containing 5% CO2. MV4-11(acute myelomonocytic leukemia cell line) and MOLM-13(acute monocytic leukemia cell line) cell lines were a kind gift from Professor Ravi Bhatia (City of Hope National Medical Center, Duarte, CA, USA). These two cell lines were cultured in Iscove's Modified Dulbecco's Medium (IMDM) supplemented with 10% (FBS). Diagnostic AML patient samples were purified by standard Ficoll‐Hypaque (Sigma-Aldrich) density centrifugation, then cultured in RPMI 1640 with 10% FBS.

Mao S., Ling Q., Pan J., Li F., Huang S., Ye W., Wei W., Lin X., Qian Y., Wang Y., Huang X., Huang J., Wang J, & Jin J. (2021). Inhibition of CPT1a as a prognostic marker can synergistically enhance the antileukemic activity of ABT199. Journal of Translational Medicine, 19, 181.

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Glioma Cell Lines and Macrophage Cultivation

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Glioma cell lines U87 and human mononuclear macrophage line (THP-1) were purchased from the Shanghai Cell Bank of the Chinese Academy of Sciences (Shanghai, China). Human microglia line HMC3 and murine glioma cell line GL261 were obtained from American Type Culture Collection (Manassas, VA, USA). The extraction of the patient-derived primary glioma cells (PGC28) were extracted as previously described.^{21 (link)} U87 and GL261 were maintained in Dulbecco’s modified Eagle’s medium (D MEM, 10566024, Gibco) supplemented with 10% fetal bovine serum (FBS, 16140071, Gibco) and 1% penicillin/streptomycin (10378016, Gibco). PGC28 and THP-1 were maintained in RPMI-1640 medium (61870036, Gibco) supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin. THP-1 monocytes were primed with 5 nM PMA (P1585, Sigma) for 48 hours to become THP1-derived macrophages. HMC3 were maintained in Minimum Essential Medium (MEM, 12561056, Gibco) supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin. All cells were cultured at 37°C with 5% CO₂.

Liu T., Zhu C., Chen X., Wu J., Guan G., Zou C., Shen S., Chen L., Cheng P., Cheng W, & Wu A. (2022). Dual role of ARPC1B in regulating the network between tumor-associated macrophages and tumor cells in glioblastoma. Oncoimmunology, 11(1), 2031499.

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Cultivation of AML Cell Lines and Primary Cells

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Human AML cell lines U937, THP‐1, Kasumi‐1, SKNO‐1, and ME‐1 were purchased from Cell Bank of Shanghai Institute of Biochemistry and Cell Biology. Primary leukemia cells from AML patients (The Affiliated DrumTower Hospital of Nanjing University Medical School, Nanjing, China) were collected using lymphocyte‐monocyte separation medium (Jingmei, Shanghai, China). Primary AML cells and AML cell lines were cultured in RPMI‐1640 medium, supplemented with 10% FBS, 100 U/mL of benzyl penicillin, and 100 U/mL of streptomycin in a humidified environment with 5% CO₂ at 37°C. All cells used were passaged in our laboratory for less than 3 months after resurrection.

Yu X., Li H., Hu P., Qing Y., Wang X., Zhu M., Wang H., Wang Z., Xu J., Guo Q, & Hui H. (2020). Natural HDAC‐1/8 inhibitor baicalein exerts therapeutic effect in CBF‐AML. Clinical and Translational Medicine, 10(4), e154.

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Cell Characterization and siRNA Transfection

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Cell lines (HepG2, Hep3B2.1-7, PLC/PRF/5, and THP-1) were purchased from the Shanghai Cell Bank, Chinese Academy of Sciences. Human liver cancer cell lines (THLE-2, SK-EP-1, SMMC7721, HCCLM3, BEL-7404, and Huh7) were obtained from the Liver Cancer Institute, Zhongshan Hospital, Fudan University (Shanghai, China). All cell lines used in this study were characterized by the cell bank based on cell morphology, post-freeze viability, isoenzyme analysis, DNA fingerprinting analysis, mycoplasma contamination testing, and bacterial and fungal contamination. HCC cell lines were cultured in Dulbecco’s modified Eagle’s medium (DMEM) containing 10% fetal bovine serum (FBS) supplemented with 100 IU/ml penicillin and 100 μg/ml streptomycin, and incubated at 37 C under a humidified atmosphere with 5% CO₂.THP-1 were cultured in 1640 containing 10% FBS. The cells were transfected with 100nM of si-Ctrl (SIGS0002902-4, Ribobio Co. Guangzhou, China) or si-TOP2A (SIGS0002902-4, Ribobio Co. Guangzhou, China) using Lipofectamine® 3000 transfection reagent (ThermoFisher Scientific, Waltham, MA) accordance with the product instructions.

Zhao H.C., Chen C.Z., Tian Y.Z., Song H.Q., Wang X.X., Li Y.J., He J.F, & Zhao H.L. (2023). CD168+ macrophages promote hepatocellular carcinoma tumor stemness and progression through TOP2A/β-catenin/YAP1 axis. iScience, 26(6), 106862.

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Macrophage Differentiation and Stimulation

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Bone marrow cells were taken from C57BL/6J mice and placed on cell culture dishes (96 mm × 22 mm; CELLTER, China) at 37 °C/5% CO₂ in DMEM (Corning, USA) containing 10% fetal bovine serum (FBS; Corning, USA). The cells differentiated into macrophages induced by granulocyte macrophage colony-stimulating factor (GM-CSF, 100 ng/mL; PeproTech, USA) for 7 days. BMDMs were placed on 12-well cell culture plates (CELLTER) for 48 h at 37 °C/5% CO₂ in DMEM containing 10% FBS. Then, mouse macrophages were cultured with LPS (from E. coli K235, 1 μg/mL for all in vitro stimulations) for the specified time.
Human monocytic cell line THP-1 was purchased from Shanghai Cell Bank, Chinese Academy of Sciences. The cells were cultured in RPMI-1640 (Hyclone, Thermo Scientific, USA) medium supplemented with 10% fetal bovine serum (FBS; Corning, USA) at 37 °C in a humidified incubator with 5% CO₂ and 95% air atmosphere. To stimulate differentiation, cells (5 × 10⁵ cells/mL) were cultured with 100 nM phorbol 12-myristate 13-acetate (PMA, Sigma-Aldrich, USA) for 24 h. Then non-attached cells were removed by aspiration and the adherent cells were washed with RPMI 1640 for three times. For cell stimulation, the adherent cells followed by 24-h incubation were further incubated with or without lipopolysaccharide for the specified time.

Yu T., Wang P., Wu Y., Zhong J., Chen Q., Wang D., Chen H., Hu S, & Wu Q. (2022). MiR-26a Reduces Inflammatory Responses via Inhibition of PGE2 Production by Targeting COX-2. Inflammation, 45(4), 1484-1495.

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