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Abet 2 touch software

Manufactured by Lafayette Instrument
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Sourced in United States, United Kingdom

The ABET II Touch software is a data acquisition and analysis tool designed for laboratory applications. It provides a user-friendly interface for collecting and processing experimental data. The software supports a range of measurement and analysis capabilities, allowing users to capture, display, and analyze data from various sources.

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Lab products found in correlation

Whiskerserver software, by Lafayette Instrument (3 mentions) Whisker server, by Lafayette Instrument (2 mentions) Env 203 1000, by Med Associates (1 mentions) Model env 203 1000, by Med Associates (1 mentions) Whisker server controller, by Campden Instruments (1 mentions)

8 protocols using abet 2 touch software

1

Operant Task Performance in Mice

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Performance in a reward task was tested using automated Bussey-Saksida Mouse Touch Screen operant chambers (Campden Instruments)40 (link) and ABET II TOUCH software (Lafayette Instrument). Throughout training and testing stages, animals had limited access to water (30 min d–1). For habituation and training, procedures outlined in the ABET II TOUCH paired discrimination task module (version 3) were followed. Animals had to touch the monitor window when a cue was presented to obtain a 7-µl water reward. After an intertrial interval of 20 s, the animal could initiate a new trial with a nose-poke of the food tray. Incorrect window touches were punished with the house light being turned on with no reward presentation for 5 s, followed by the regular 20-s intertrial interval until the animal could initiate a new trial. A learning session lasted until the mouse initiated 30 trials or a maximum of 30 min. The animals were tested for the operant reward-seeking performance in the same task during two 30-min sessions with and without laser stimulation on consecutive days.
Stegemann A., Liu S., Retana Romero O.A., Oswald M.J., Han Y., Beretta C.A., Gan Z., Tan L.L., Wisden W., Gräff J, & Kuner R. (2023). Prefrontal engrams of long-term fear memory perpetuate pain perception. Nature Neuroscience, 26(5), 820-829.
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2

Assessing Cognitive Function in Chemotherapy-Treated Mice

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At 11 weeks of age (8 weeks after the start of chemotherapy), animals were food-restricted to 85–90% of their free feeding body weight over the course of a week and animals were pre-exposed to the reinforcer (Yoo-hoo, Dr. Pepper Snapple Group Inc., Plano, TX, USA) for 24 hours in the home cage. Operant testing was carried out in Bussey-Saksida Mouse Touch Screen operant chambers (Lafayette Instrument, Lafayette, IN, USA) running ABET II Touch software as previously described (Makinson et al., 2019 (link); Smith et al., 2020 (link)). Basic cognition was assessed using a fixed ratio (FR) 1 schedule, and motivation was assessed using a progressive ratio (PR) schedule. Following FR1 and PR, executive function was assessed.
Konsman J.P., Laaker C.J., Lloyd K.R., Hiltz A., Smith B.L., Smail M.A, & Reyes T.M. (2021). Translationally relevant mouse model of early life cancer and chemotherapy exposure results in brain and small intestine cytokine responses: a potential link to cognitive deficits. Brain, behavior, and immunity, 99, 192-202.
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3

Automated Touchscreen Operant Chambers for Behavioral Tasks

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All behavioral tasks were carried out using four automated Bussey-Saksida style touchscreen-based operant chambers (Lafayette Instruments, Lafayette, IL) consisting of four modular testing chambers housed individually within a sound- and light-attenuating cubicle equipped with an overhead camera to monitor task-related locomotion. Inside each chamber, a reward collection magazine connected to a liquid dispenser pump was situated opposite a touch-sensitive monitor. Depending on the task, the touchscreen monitor was divided into one, two or five response windows by interchanging different black Perspex masks. Each response window was 4.0 × 4.0 cm regardless of how many response windows each mask contained. The collection magazine contained an LED illuminated coincident with delivery of a liquid reward (Nesquik® strawberry-flavored milk) and a tone. Infrared beam arrays distributed in front of the touchscreen, reward collection port and across the intervening floor, were used to monitor locomotor activity. Different training schedules, depending on the task, were designed and data were analyzed using ABET II Touch software (Lafayette Instruments), while inputs and outputs from all four chambers were controlled by WhiskerServer software (Lafayette Instruments).
Hussein A., Tielemans A., Baxter M.G., Benson D.L, & Huntley G.W. (2022). Cognitive deficits and altered cholinergic innervation in young adult male mice carrying a Parkinson’s disease Lrrk2G2019S knockin mutation. Experimental neurology, 355, 114145.
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4

Primate Behavioral Testing in Enclosures

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Monkeys were individually housed in well-ventilated, stainless steel chambers. Each chamber was equipped with a custom-made, stainless steel screen enclosure (Lafayette Instrument, Lafayette, USA) that was mounted on the chamber front to provide access to a 15″ touch-sensitive screen (Model 1537L; Elo TouchSystems, Menlo Park, CA). A pellet dispenser (ENV-203–1000; Med Associates, St Albans, VT) was mounted on a shelf above the chamber. All experimental events and data were collected using custom programming in ABET II Touch software (Lafayette Instrument,) in tandem with Whisker server (Cambridge University, UK).
Schwienteck K.L., Negus S.S, & Banks M.L. (2019). Sex differences in the effectiveness of buprenorphine to decrease rates of responding in rhesus monkeys. Behavioural pharmacology, 30(4), 358-362.
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5

Touchscreen Visual Discrimination in Monkeys

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Monkeys were housed individually in well-ventilated, stainless steel chambers (66×76×94 cm) that also served as experimental chambers. Each chamber was equipped with a custom-made, stainless steel screen enclosure (Lafayette Instrument, Lafayette, USA), which was mounted on the front wall of the chamber to provide access to a 15″ touch-sensitive screen (33.6×26.4 cm Model 1537L; Elo TouchSystems, Menlo Park, CA). Each chamber was also equipped with a pellet dispenser (Model ENV-203-1000; Med Associates, St Albans, VT) mounted on a shelf above the chamber. All experimental events and data were collected using custom programming in ABET II Touch software (Lafayette Instrument, Lafayette, USA) in tandem with a Whisker server (Cambridge University, UK) controlled the touch-sensitive apparatus. Touchscreen stimuli were made in Microsoft PowerPoint for Mac 2011 using the hue-saturation-brightness slider. Sample and comparison stimuli were different shades of gray constructed by adjusting the hue and saturation to 0.0 and varying brightness/intensity. Brightness was set at 1.8% (black) and 100.0% (white) throughout the present study.
Hutsell Blake A, & Banks Matthew L. (2015). Effects of environmental and pharmacological manipulations on a novel delayed nonmatching-to-sample ‘working memory’ procedure in unrestrained rhesus monkeys. Journal of neuroscience methods, 251, 62-71.
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6

Rodent Touchscreen Cognitive Assays

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5CSRTT and 2CSRTT (see also Supplementary Figure 1) was conducted in eight Bussey–Saksida operant chambers with a touchscreen system (Lafayette Instruments, Lafayette, IL) following procedures previously described in Norman et al. (2021b) (link). Dimensions are as follows: a black plastic trapezoid [walls 20 cm high × 18 cm wide (at screen-magazine) × 24 cm wide (at screen) × 6 cm wide (at magazine)]. Stimuli were displayed on a touch-sensitive screen (12.1 inch, screen resolution 600 × 800) divided into five response windows by a black plastic mask (4.0 × 4.0 cm, positioned centrally with windows spaced 1.0 cm apart, 1.5 cm above the floor) fitted in front of the touchscreen. For the 5CSRTT, all five response windows were available, but during the 2CSRTT, three of the outer response windows were masked and only two adjacent center windows were available. Schedules were designed and data was collected and analyzed using ABET II Touch software (v18.04.17, Lafayette Instrument). The inputs and outputs of the multiple chambers were controlled by WhiskerServer software (v4.7.7, Lafayette Instrument).
Norman K.J., Bateh J., Maccario P., Cho C., Caro K., Nishioka T., Koike H, & Morishita H. (2022). Frontal-Sensory Cortical Projections Become Dispensable for Attentional Performance Upon a Reduction of Task Demand in Mice. Frontiers in Neuroscience, 15, 775256.
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7

Touchscreen-Based 5-Choice Serial Reaction Time Task

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5CSRTT (See also Fig. S1) was conducted in eight Bussey–Saksida operant chambers with a touchscreen system (Lafayette Instruments, Lafayette, IL) following procedures previously described in (Nabel et al., 2020 (link)). Dimensions are as follows: a black plastic trapezoid (walls 20 cm high × 18 cm wide (at screen-magazine) × 24 cm wide (at screen) × 6 cm wide (at magazine)). Stimuli were displayed on a touch-sensitive screen (12.1 inch, screen resolution 600 × 800) divided into five response windows by a black plastic mask (4.0 × 4.0 cm, positioned centrally with windows spaced 1.0 cm apart, 1.5 cm above the floor) fitted in front of the touchscreen. Schedules were designed and data were collected and analyzed using ABET II Touch software (v18.04.17, Lafayette Instrument). The inputs and outputs of the multiple chambers were controlled by WhiskerServer software (v4.7.7, Lafayette Instrument).
Norman K.J., Riceberg J.S., Koike H., Bateh J., McCraney S.E., Caro K., Kato D., Liang A., Yamamuro K., Flanigan M.E., Kam K., Falk E.N., Brady D.M., Cho C., Sadahiro M., Yoshitake K., Maccario P., Demars M.P., Waltrip L., Varga A.W., Russo S.J., Baxter M.G., Shapiro M.L., Rudebeck P.H, & Morishita H. (2021). Post-error Recruitment of Frontal-Sensory Cortical Projections Promotes Attention in Mice. Neuron, 109(7), 1202-1213.e5.
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8

Touchscreen Cognitive Behavioral Testing in Rats

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Rats were housed in pairs in open-top cages under reverse light cycle (12h:12h, lights off at 0700 hours). Behavioural testing was conducted during the dark (active) phase of the cycle. Rats were handled and began training in the touchscreen chambers at 8 weeks of age.
Rats were food restricted to ~85-90% free-feeding body weight only during touchscreen testing. Prior to surgery and at all other times between behavioural testing, rats received ad libitum access to food. Water was available ad libitum at all times throughout the experiment.
Apparatus: Cognitive behavioural training and testing was performed in the Bussey-Saksida rat touchscreen chambers (Campden Instruments Ltd, UK). Detailed description of this apparatus has been provided elsewhere (eg., [16] ). Chambers were controlled using the ABET II Touch software (Lafayette Instruments) using the WhiskerServer Controller (Campden Instruments Ltd, UK).
Ermine C.M., Nithianantharajah J., O'Brien K., Kauhausen J.A., Frausin S., Oman A., Parsons M.W., Brait V.H., Brodtmann A, & Thompson L.H. (2021). Hemispheric cortical atrophy and chronic microglial activation following mild focal ischemic stroke in adult male rats. Journal of neuroscience research, 99(12).
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