Qsense analyzer

Manufactured by Biolin Scientific

Sourced in Sweden

The Qsense Analyzer is a versatile instrument for surface interaction analysis. It measures changes in frequency and dissipation of a quartz crystal sensor, providing insights into surface properties, adsorption, and viscoelastic changes in real-time.

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Lab products found in correlation

Sio2 coated quartz crystal sensors, by Biolin Scientific (2 mentions) Qsx303, by Biolin Scientific (1 mentions) S8tlger, by Bruker (1 mentions) σigma hd, by Zeiss (1 mentions) Nrs 4100, by Jasco (1 mentions) D8 advance, by Bruker (1 mentions) Qsx301, by Biolin Scientific (1 mentions) Qtools, by Biolin Scientific (1 mentions)

12 protocols using qsense analyzer

Protein Cluster Analysis via QCM-D

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QCM-D measurements were performed
using a Qsense analyzer (Biolin Scientific). Measurements were performed
at 22 °C and operated with four parallel flow chambers, using
two Ismatec peristaltic pumps with a flow rate of 100 μL/min.
Throughout this work, the fifth overtone was used for the normalized
frequency (Δf₅) and dissipation
(ΔD₅). SiO₂-coated sensors
(QSX303, Biolin Scientific) were used. During every rHA protein cluster
addition, solutions were recycled.

Di Iorio D., Verheijden M.L., van der Vries E., Jonkheijm P, & Huskens J. (2019). Weak Multivalent Binding of Influenza Hemagglutinin Nanoparticles at a Sialoglycan-Functionalized Supported Lipid Bilayer. ACS Nano, 13(3), 3413-3423.

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QSense Analyzer-Based Sensor Cleaning

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The experiments were conducted using QSense^® Analyzer (4-channel system) from Biolin Scientific (Gothenburg, Sweden). Moreover, QSensor QSX 309 Al₂O₃ (Aluminum oxide) was used in the experiments. Before each experiment, the alumina sensor was cleaned. First, the sensor with 99% ethanol was sonicated for 15 min. Subsequently, it was rinsed with Mill-Q water and carefully blow-dried with nitrogen gas. Next, the sensor was exposed to UV/ozone treatment for 10 min to remove hydrocarbon and organic contaminants. After the flow modules were installed, the four-sensor chamber was inverted to make the chip surface face down. The liquid was made to flow under the surface to ensure that the particles are absorbed, rather than being made to deposit by gravity onto the surface. The flow rate was 0.15 mL/min for all the experiments, and the temperature was set to 22 °C. Measurement data for frequency and dissipation were gathered simultaneously at several overtones (n = 1, 3, 5, 7, 9, 11 or 13). Owing to the stable and sensitive responses, the third overtones were used to present the results. [23 (link),24 (link),38 (link),39 (link)]

Ma X., Sun X., Chang M., Liu Q., Dong X., Fan Y, & Chen R. (2023). Adsorption of Different Ionic Types of Polyacrylamide on Montmorillonite Surface: Insight from QCM-D and Molecular Dynamic Simulation. Molecules, 28(11), 4417.

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Supported Lipid Bilayer Formation and Synmin Binding

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QCMD measurements were carried out following the previous report^{23 (link)}. Prior to each measurement, silicon dioxide (SiO2)-coated quartz crystal sensors (Biolin Scientific, Gothenburg, Sweden) were treated with a 3:1 mixture of sulfuric acid and hydrogen peroxide (piranha-solution). Subsequently, sensors were rinsed with ultrapure water, dried under a stream of nitrogen, and mounted in the flow modules of the Qsense Analyzer (Biolin Scientific). After baseline stabilization, supported lipid bilayers (SLBs) formation was induced through constant injection (flow rate: 0.15 mL/min) of a 1 mg/mL mixture of SUVs solution (prepared as described in the method section for ATPase assay), in the TK buffer (20 mM Tris-HCl pH 7.5, 150 mM KCl), spiked with 5 mM CaCl2. The formed SLBs were washed with TK buffer until no frequency change was observed. Then, 150 µl of the 5 µM of each synMinE variant in TK buffer was flown over the sensor at 0.15 ml/min and the change in frequency was monitored at overtone F9. The measured frequency was normalized by averaging the value of 5 consequent measurements at each time point, and then the change in frequency was determined by subtracting the maximum value (as baseline) from the minimum value (as dropped frequency).

Kohyama S., Frohn B.P., Babl L, & Schwille P. (2024). Machine learning-aided design and screening of an emergent protein function in synthetic cells. Nature Communications, 15, 2010.

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Multimodal Characterization of Electrode Surfaces

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X-ray diffraction (XRD)
measurements were
performed with a Bruker D8 Advance diffractometer. X-ray fluorescence
(XRF) experiments were carried out with an S8TLGER (Bruker). The morphologies
of the modified electrode surfaces were observed using field emission
scanning electron microscopy (FE-SEM, ΣIGMA-HD, ZEISS, Germany).
Raman spectroscopy was performed using a laser micro-Raman spectrometer
(JASCO NRS-4100, Japan) with an excitation wavelength of 532.0 nm
laser with a working distance on a ×100 lens. The Raman spectra
of MLN, MLN/TYR, and MLN/AO/TYR were recorded by depositing the samples
on a GCE rod (3 mm in diameter and 5 mm in length). All electrochemical
measurements such as cyclic voltammetry (CV), constant-potential amperometry,
and electrochemical impedance spectroscopy (EIS) were performed with
a CHI 660E workstation (Shanghai Chenhua, China). Quartz crystal microbalance
with dissipation (QCM-D) measurements were performed with a Q-Sense
analyzer (Biolin Scientific) equipped with a MoS₂-coated
sensor tip.

Zhang Y., Wang Y., Zhang Z., Sobhy A., Sato S., Uchida M, & Hasebe Y. (2021). Natural Molybdenite- and Tyrosinase-Based Amperometric Catechol Biosensor Using Acridine Orange as a Glue, Anchor, and Stabilizer for the Adsorbed Tyrosinase. ACS Omega, 6(21), 13719-13727.

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Antibody Adsorption on Bio-based Materials

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To better understand the interactions occurring between the bio-based materials and the csELISA antibodies, adsorption of the Pf, Pv210, and Pv247 antibodies on model films was performed in a QSense Analyzer from Biolin Scientific (Västra Frölunda, Västra Frölunda, Sweden). Model films were prepared over polyethyleneimine (PEI) precoated gold quartz sensors (5 MHz base resonance) by spin coating 180 μl of 0.1% solutions of the bio-based materials at 3,000 rpm for 1 min in a WS-650MZ-23NPPB spin coater by Laurell Technologies Corporation (North Wales, PA, USA). For measurements, the sensors were first stabilized on PBS buffer (pH 7.4) at 25°C with a flow of 50 μl/min. Then, 4 μl/ml of Pf or 8 μl/ml of Pv210/Pv247 antibody solutions were poured into the channel and allowed to interact with the surfaces until no changes were detected. Finally, a rinse with PBS was performed to remove the excess material. Data analysis was performed with DFind Software from Biolin Scientific (Västra Frölunda, Sweden) using the Composite Sauerbrey model and the software’s predetermined density values (Gomez-Maldonado et al. 2022 ).

, & Hurley J. (2001). Cost and effect. CMAJ: Canadian Medical Association Journal, 165(8), 1073-1074.

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Quantitative Analysis of pDNA and RLS Multilayer Deposition

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The stepwise deposition process of pDNA and RLS was observed timely using the quartz crystal microbalance with dissipation assay (QCM-D, Qsense Analyzer, Biolin Scientific). Firstly, P-coating was prepared on an Aurum (Au)-coated quartz crystal as the same method described in the Titanium substrate preparation and phase-transition lysozyme (P) coating section. Then it was installed in the test cell of the instrument and washed thoroughly with ultrapure (UP) water to obtain a stable baseline. Afterward, the configured pDNA solution (100 µg/ml) and RLS solution (1.0 mg/ml) were pumped into the channel alternatively at a controlled flux speed of 30 μl/min. At each change interval, the unbound polyelectrolyte on the substrate was thoroughly rinsed with UP water for 3–5 min. The deposition process was repeated for six cycles. The growth behavior of multilayer coatings was monitored by recording frequency/dissipation-time curves. Finally, the mass and thickness of the deposited (pDNA/RLS)₆ films were quantified using Dfind Smartfit modeling in the affiliated Dfind software from Q-Sense.

He T., Wang Y., Wang R., Yang H., Hu X., Pu Y., Yang B., Zhang J., Li J., Huang C., Jin R., Nie Y, & Zhang X. (2023). Fibrous topology promoted pBMP2-activated matrix on titanium implants boost osseointegration. Regenerative Biomaterials, 11, rbad111.

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Real-time Monitoring of Collagen Coating

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The QSense Analyzer instrument (Biolin Scientific AB, Sweden) is a powerful tool to efficiently monitor the real-time change in the grafted molecule mass of the (rhCol III/PDA-PEI)_n coating at room temperature. Briefly, the PDA-PEI film was predeposited on gold-coated quartz crystals (Φ 19.97 mm, QSX 301, Biolin Scientific AB, Sweden) as mentioned earlier before monitoring the construction process of the (rhCol III/PDA-PEI)_n coating, and assumed to be a control group. Then, the reaction solution was passed into the chamber at a flow rate of 50 μl/min, and the concentration of the above solution was consistent with the experimental concentrations^{25 (link),60 (link)}. The data analysis was conducted with Dfind Smartfit modeling in QSense Dfind software (QSoft401).

Wu H., Yang L., Luo R., Li L., Zheng T., Huang K., Qin Y., Yang X., Zhang X, & Wang Y. (2024). A drug-free cardiovascular stent functionalized with tailored collagen supports in-situ healing of vascular tissues. Nature Communications, 15, 735.

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Quantifying Peptide Adsorption on Polystyrene

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To evaluate peptide adsorption onto PS, a QCM-D system (Q-Sense Analyzer, Biolin Scientific, Sweden) and PS-coated QCM-D sensors (QSX 310, Q-Sense, Sweden) were used. The crystal sensors were 14 mm diameter, 10 nm layer thickness, and <3 nm surface roughness. The adsorption from the laccase formulation onto the crystal surface contact was monitored for the overtones (n = 1, 3, 5, 7,9,11 and 13) at 25 °C. To remove surface contaminants before use, crystals were placed in a UV-ozone chamber for 15 min. For the measurement, the testing solutions were pumped through the flow cells at a constant flow rate of 100 μL min⁻¹ in the following order: buffer solution for 30 min (baseline), laccase maltodextrin suspension, peptide solution and buffer solution (washing step). Resonance frequency changes Δf determined by adsorbed mass were recorded and analyzed using commercial software (QSoft, supplier Q-Sense, Sweden). The Sauerbrey model was used to calculate the mass, due to the small changes on the dissipation coefficient (ΔD is close to zero). Measurements were repeated at least three times and a representative data is shown.

Corrales-Ureña Y.R., Souza-Schiaber Z., Lisboa-Filho P.N., Marquenet F., Michael Noeske P.L., Gätjen L, & Rischka K. (2020). Functionalization of hydrophobic surfaces with antimicrobial peptides immobilized on a bio-interfactant layer. RSC Advances, 10(1), 376-386.

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Supported Lipid Bilayer Formation and Protein Binding Assay

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Prior to each measurement, silicon dioxide (SiO₂)-coated quartz crystal sensors (Biolin Scientific, Gothenburg, Sweden) were treated with a 3:1 mixture of sulfuric acid and hydrogen peroxide (piranha-solution). Subsequently, sensors were rinsed with ultrapure water, dried under a stream of nitrogen, and mounted in the flow modules of the Qsense Analyzer (Biolin Scientific, Gothenburg, Sweden). After baseline stabilization, supported lipid bilayer formation (SLB) was induced through constant injection (flow rate: 0.15 mL/min) of a 1 mg/mL mixture of small unilamellar vesicles (DOPC/DOPC, 70:30 mol %) in buffer (25 mM Tris- HCl pH 7.5, 150 mM KCl, 5 mM MgCl₂), spiked with 5 mM CaCl₂. The formed bilayer was washed with buffer until no frequency change was observed. Then, 150 µl of sample was flown over the sensor at 0.1 ml/min and the change in frequency monitored at overtone F9.

Babl L., Merino-Salomón A., Kanwa N, & Schwille P. (2022). Membrane mediated phase separation of the bacterial nucleoid occlusion protein Noc. Scientific Reports, 12, 17949.

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QCM Analysis of Nanoparticle Adsorption

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For quartz crystal microbalance (QCM) analysis, Au sensors (Biolin Scientific) were modified with amino as literature [46 (link)]. In brief, Au QCM sensors were treated in ultraviolet for 30 min and exposed to 1 mL dopamine hydrochloride (0.2 mg/mL) in Tris-based buffer (10 mM, pH = 8.5) at 20 °C for 45 min. Then the Au sensors were submerged in polyethyleneimine (PEI, 20 mg/mL) Tris-based buffer (10 mM, pH = 8.5) at 20 °C for 30 min. After that, the sensors were washed with distilled water three times, followed by drying with nitrogen. The QCM-D instrument (Q-Sense Analyzer, Biolin Scientific, Gothenburg, Sweden) was adopted for relative determination. After heating the sensors to 37 °C, baseline measurements were obtained for the dry sensors and the sensors under distilled water flowing at 20 μL/min. NPs solution (0.5 mg/mL) was introduced to the sensors after the acquisition of distilled water baseline and measurements of resonant frequency and dissipation factor were recorded over 1.5 h, followed by 0.5 h rinsing with water. Modeling of raw data to obtain deposited masses and shear modulus was completed with QTools (Biolin Scientific).

Yu Y., Li S., Yao Y., Shen X., Li L, & Huang Y. (2022). Increasing stiffness promotes pulmonary retention of ligand-directed dexamethasone-loaded nanoparticle for enhanced acute lung inflammation therapy. Bioactive Materials, 20, 539-547.

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