4 μl of 1 μM assembled clathrin cages was applied to a glow-discharged formvar carbon 300-mesh copper grid (Agar Scientific) and allowed to adsorb for 1 minute. The sample was removed by blotting and 4 μl of 2% w/v uranyl acetate (UA) rapidly applied to the grid. This was incubated at room temperature for 1 minute to stain the sample after which the UA removed by blotting. Grids were air dried at room temperature before transfer to the microscope. Samples were imaged using a JEOL 2010F FEG transmission electron microscope with a Gatan Ultrascan 2000™ camera, at an accelerating voltage of 200kV.
Formvar carbon 300 mesh copper grid
Manufactured by Agar Scientific
Formvar carbon 300-mesh copper grid is a laboratory equipment used for specimen preparation in electron microscopy. It consists of a copper mesh grid coated with a thin film of formvar, a transparent polymer, and a layer of carbon. This grid provides a stable and uniform support for samples to be imaged under an electron microscope.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using formvar carbon 300 mesh copper grid
1
Visualizing Clathrin Caged Nanostructures
2
Visualizing Clathrin Caged Nanostructures
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!