Iodoacetamide iam

Manufactured by Thermo Fisher Scientific

Iodoacetamide (IAM) is a chemical compound commonly used in biochemistry and molecular biology laboratories. It is a colorless, crystalline solid that serves as a reagent for the modification and labeling of cysteine residues in proteins. IAM functions by alkylating sulfhydryl groups, which can be useful for various experimental procedures and analyses.

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Lab products found in correlation

Formic acid, by Thermo Fisher Scientific (6 mentions) Dithiothreitol (dtt), by Thermo Fisher Scientific (4 mentions) Acetonitrile, by Thermo Fisher Scientific (4 mentions) Methanol, by Thermo Fisher Scientific (4 mentions) Glu1 fibrinopeptide b lock mass standard, by Waters Corporation (2 mentions) Ammonium bicarbonate, by Avantor (2 mentions) Deep well 1 ml 96 well plate protein lobind, by Eppendorf (2 mentions) Recombinant mouse hexb protein his tag, by MyBioSource (2 mentions) Enzyme activity assay kit for β n acetyl glucosaminidase, by Merck Group (2 mentions) Recombinant nag enzyme, by New England Biolabs (2 mentions) Instant pc kit, by Agilent Technologies (2 mentions) Tris hcl buffer, by Thermo Fisher Scientific (2 mentions) Sequencing grade modified trypsin, by Promega (2 mentions) Chymotrypsin, by Promega (1 mentions) Rapigest surfactant, by Waters Corporation (1 mentions) Pngase f glycosidase, by Promega (1 mentions) Alcohol dehydrogenase, by Merck Group (1 mentions) Myoglobin, by Merck Group (1 mentions)

Close spelling variants of this product

Iodoacetamide

4 protocols using iodoacetamide iam

HA-SS-np Stability Characterization

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HA-SS-np materials were produced at the Vaccine Production Program Laboratory (Gaithersburg, MD). HA-SS-np formulated in one of the five formulation buffers (containing sodium phosphate, sodium chloride, and sucrose, pH 7.2) was used for investigational purposes, and a HA-SS-np interim reference material (in 1x PBS) was used as a control. Reagents included LC-MS grade 0.1% formic acid in water (mobile phase A), 0.1% formic acid acetonitrile (mobile phase B), and ammonium bicarbonate that were purchased from JT Baker (Phillipsburg, NJ). RapiGest^™ surfactant and [Glu1]-Fibrinopeptide B lock mass standard were purchased from Waters (Milford, MA). Formic acid, urea, and Zeba^™ desalting columns (7K MWCO) were purchased from Pierce (Rockford, IL). Dithiothreitol (DTT) and iodoacetamide (IAM) were purchased from ThermoFisher Life Technologies (Grand Island, NY) and Sigma Aldrich (St. Louis, MO), respectively. Chymotrypsin and PNGase F glycosidase were purchased from Promega (Madison, WI); Lys-C was purchased from New England Biolabs (Ipswich, MA). Method details of the stability-indicating analyses (rCGE and SEC) are described in the online supporting information.

Schneck N.A., Ivleva V.B., Rosales-Zavala E., Wang X., Gollapudi D., Cooper J.W, & Lei Q.P. (2019). Using LC-MS to Identify Clipping in Self-Assembled Nanoparticles During Vaccine Development. Journal of the American Society for Mass Spectrometry, 30(12), 2576-2579.

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Env Trimer Production and Characterization

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Env trimer materials were produced in-house at the Vaccine Production Lab in NIH (Bethesda, MD) and based on the BG505.DS.SOSIP.664 construct. Purified trimeric Env material was produced in the CHO cell line and formulated in either 1×PBS or final formulation buffer. For the LC-MS analysis, reagents included high-purity LC-MS grade water and acetonitrile containing formic acid (mobile phases) and ammonium bicarbonate, which were purchased from J.T. Baker (Phillipsburg, NJ). [Glu1]-Fibrinopeptide B lock mass standard was purchased from Waters (Milford, MA). Formic acid, urea and Zeba^™ spin desalting columns (7 K MWCO) were purchased from Pierce (Rockford, IL). Dithiothreitol (DTT) and iodoacetamide (IAM) were purchased from ThermoFisher Life Technologies (Grand Island, NY) and Sigma Aldrich (St Louis, MO), respectively. Rapid PNGase F glycosidase (reducing and non-reducing formats) was purchased from New England Biolabs (Ipswich, MA).

Kam-Morgan L.N., Smith-Gill S.J., Taylor M.G., Zhang L., Wilson A.C, & Kirsch J.F. (1993). High-resolution mapping of the HyHEL-10 epitope of chicken lysozyme by site-directed mutagenesis.. Proceedings of the National Academy of Sciences of the United States of America, 90(9), 3958-3962.

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Quantitative Proteomics Using SIL Peptides

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Dithiothreitol (DTT) and iodoacetamide (IAM) were purchased from Thermo Pierce (Rockford, IL). Tris-HCl buffer and high-performance liquid chromatography (HPLC) grade solvents, methanol, acetonitrile (ACN), formic acid (FA), were purchased from Thermo Fisher Scientific (Waltham, MA). The custom stable isotopic labeled (SIL) surrogate peptide (GILIDTSR, AQUA Ultimate grade) with 13C and 15N labeled arginine (R*), and non-labeled surrogate peptide were synthesized from Thermo Fisher Scientific (Waltham, MA). The mAbs are from the pipeline of Merck & Co., Inc., Kenilworth, NJ, USA. Sequencing grade modified trypsin was purchased from Promega (Madison, WI). Ammonium bicarbonate and urea were purchased from JT Baker (Center Valley, PA). Deep well 1-mL 96 well plate (Protein LoBind) was purchased from Eppendorf (Hauppauge, NY). Recombinant mouse HEXB protein (His Tag) was obtained from MyBioSource (San Diego, CA). Recombinant proteins alcohol dehydrogenase (ADH) from yeast, carbonic anhydrase ii (CA2) from Bos Taurus, myoglobin (MB) from equine heart, phosphorylase-b (PYGM) from rabbit muscle and enolase (ENL) from baker's yeast, and enzyme activity assay kit for β-N-Acetyl-glucosaminidase (NAG) were purchased from Sigma (St. Louis, MO).
Recombinant NAG enzyme was from New England Biolabs (Beverly, MA). Instant PC kit was from Agilent (Palo Alto, CA).

Li X., An Y., Liao J., Xiao L., Swanson M.R., Martinez‐Fonts K., Pavon J.A., Sherer E.C., Jawa V., Gao X., Letarte S., & Richardson D.D. (2020). Identification and Characterization of a Residual Host Cell Protein Hexosaminidase B Associated with N-glycan Degradation During the Stability Study of a Therapeutic Recombinant Monoclonal Antibody Product.

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Quantitative Proteomics Protocol

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Dithiothreitol (DTT) and iodoacetamide (IAM) were purchased from Thermo Pierce (Rockford, IL). Tris–HCl buffer and high‐performance liquid chromatography (HPLC) grade solvents, methanol, acetonitrile (ACN), formic acid (FA), were purchased from Thermo Fisher Scientific (Waltham, MA). The custom stable isotopic labeled (SIL) surrogate peptide (GILIDTSR, AQUA Ultimate grade) with 13C and 15N‐labeled arginine (R*), and nonlabeled surrogate peptide were synthesized from Thermo Fisher Scientific. Sequencing grade modified trypsin was purchased from Promega (Madison, WI). Ammonium bicarbonate and urea were purchased from JT Baker (Center Valley, PA). Deep well 1‐ml 96‐well plate (Protein LoBind) was purchased from Eppendorf (Hauppauge, NY). Recombinant mouse HEXB protein (His Tag) was obtained from MyBioSource (San Diego, CA). Recombinant proteins alcohol dehydrogenase (ADH) from yeast, carbonic anhydrase ii (CA2) from Bos Taurus, myoglobin (MB) from equine heart, phosphorylase‐b (PYGM) from rabbit muscle and enolase (ENL) from baker's yeast, and enzyme activity assay kit for β‐N‐acetyl‐glucosaminidase (NAG) were purchased from Sigma (St. Louis, MO). Recombinant NAG enzyme was from New England Biolabs (Beverly, MA). Instant PC kit was from (Agilent, formerly ProZyme, Palo Alto, CA).

Li X., An Y., Liao J., Xiao L., Swanson M., Martinez‐Fonts K., Pavon J.A., Sherer E.C., Jawa V., Wang F., Gao X., Letarte S, & Richardson D.D. (2021). Identification and characterization of a residual host cell protein hexosaminidase B associated with N‐glycan degradation during the stability study of a therapeutic recombinant monoclonal antibody product. Biotechnology Progress, 37(3), e3128.

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