Gl7 fitc gl 7

Manufactured by BD

GL7-FITC (GL-7) is a fluorescently labeled reagent used in flow cytometry applications. It binds to a specific antigen on the surface of cells. The FITC (Fluorescein Isothiocyanate) fluorescent label allows for the detection and analysis of cells expressing the target antigen.

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Lab products found in correlation

Facsdiva software, by BD (1 mentions) Lsr 2, by BD (1 mentions) Cd4 af700, by BioLegend (1 mentions) B220 bv605, by BioLegend (1 mentions) Cd95 pecy7, by BD (1 mentions) 2 methylbutane, by Thermo Fisher Scientific (1 mentions) Colorfrost plus microscope slides, by Thermo Fisher Scientific (1 mentions) Cd86 pe gl 1, by BD (1 mentions)

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GL7-FITC (13 citations) FITC-GL-7 (2 citations)

2 protocols using gl7 fitc gl 7

Mouse Splenocyte Flow Cytometry

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Flow cytometric analysis of total mouse splenocytes was performed using the following antibodies: B220-BV605 (RA3–6B2), CD4-AF700 (RMP4–5), CD44-APC (IM7), CD62L-PECy7 (MEK-14), PD1-PE (29F.1A12), NK1.1-biotin (PK136), Ly6G-PB (1A8), MHCII-PE-Cy7 (M5/114.15.2) (Biolegend, Inc.). GL7-FITC (GL-7), CD95- PeCy7, CXCR5-biotin (2G8), IFNγR-biotin (GR20), STAT1-PE (1/STAT1), CD11b-AF700 (M1/70), CD11c-FITC (HL3) (BD Biosciences). Ly6C- APC (HK1.4), PDC-PE (eBio927), CD8α (clone 53–6.7) (eBioscience). All cells were stained with fixable viability dye-eFluor780 (Invitrogen) prior to surface staining. Stained cells were analyzed using the BD LSR II flow cytometer (BD Biosciences). Data were acquired using FACSDiva software (BD Biosciences) and analyzed using FlowJo software (Tree Star).

Chodisetti S.B., Fike A.J., Domeier P.P., Singh H., Choi N.M., Corradetti C., Kawasawa Y.I., Cooper T.K., Caricchio R, & Rahman Z.S. (2020). Type II but not type I interferon signaling is indispensable for TLR7-promoted development of autoreactive B cells and systemic autoimmunity. Journal of immunology (Baltimore, Md. : 1950), 204(4), 796-809.

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Cryosectioning and Immunofluorescence Analysis of Splenic Tissue

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Splenic tissue was first embedded in Tissue-Tek O.C.T. compound (Sakura) and snap frozen over liquid nitrogen in 2-Methylbutane (Fisher Scientific). Five-μm sections were cut using a cryostat (Hacker Instruments and Industries), mounted on ColorFrost Plus Microscope Slides (Thermo Fisher Scientific) and fixed in cold acetone for 20 min. Sections were stained with following Abs: GL-7-FITC (GL-7; BD Biosciences), IgD-APC (11–26c2a; BD Biosciences), CD86- PE (GL-1; BD Biosciences), or FDC-M1- Purified (FDC-M1; BD Biosciences). For active caspase 3/7 detection, we performed FAM-FLICA on unfixed splenic sections according to manufacturer instructions (ImmunoChemistry Technologies).

Fike A.J., Chodisetti S.B., Wright N.E., Bricker K.N., Domeier P.P., Maienschein-Cline M., Rosenfeld A.M., Luckenbill S.A., Weber J.L., Choi N.M., Prak E.T., Mandal M., Clark M.R, & Rahman Z.S. (2023). STAT3 signaling in B cells controls germinal center zone organization and recycling. Cell reports, 42(5), 112512.

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