Triton (1%) lysis buffer was used to lyse the fibroblast cells after compound treatment, and then 20% SDS sample buffer was used to denature the proteins at 100 °C for 10 min. Bis-Tris gel (10%, Life Tech) was used for running gels and Trans-Blot Turbo PVDF kit (Bio-Rad) was used to transfer membranes. GCase antibody (Sigma-Aldrich) and GAPDH primary antibodies were incubated with the membranes overnight, then the incubated membranes were treated with the secondary antibody (Peroxidase-AffiniPure Goat Anti-Rabbit/Mouse IgG (H+L), Jackson Immunoresearch Lab) for 30 min. Chemidoc MP system (Bio-Rad) was used to scan the membranes and analyze the imaging.
Peroxidase affinipure goat anti rabbit mouse igg h l
Manufactured by Jackson ImmunoResearch
Sourced in United States
Peroxidase-AffiniPure Goat Anti-Rabbit/Mouse IgG (H+L) is a secondary antibody conjugated with the enzyme peroxidase. It is designed to detect and bind to rabbit or mouse immunoglobulin G (IgG) antibodies in a laboratory setting.
Automatically generated - may contain errors
Lab products found in correlation
Bis tris gel, by Thermo Fisher Scientific (2 mentions)
Trans blot turbo pvdf kit, by Bio-Rad (2 mentions)
Gcase antibody, by Merck Group (2 mentions)
Chemidoc mp system, by Bio-Rad (2 mentions)
Gapdh primary antibody, by Merck Group (1 mentions)
Anti srsf5, by Abcam (1 mentions)
Anti hoxd8, by Santa Cruz Biotechnology (1 mentions)
Superpico ecl chemiluminescence kit, by Vazyme (1 mentions)
Pvdf membrane, by Merck Group (1 mentions)
Ripa lysis buffer, by Beyotime (1 mentions)
Chemidoc xrs imaging system, by Bio-Rad (1 mentions)
Anti gapdh, by ABclonal (1 mentions)
3 protocols using peroxidase affinipure goat anti rabbit mouse igg h l
1
Western Blot Analysis of GCase
2
Western Blot Analysis of GCase Protein
3
Western Blot Protein Expression Analysis
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Total cellular proteins were extracted using RIPA lysis buffer (Beyotime), separated via electrophoresis on a 10% SDS separation gel (YEASEN, China), and transferred to PVDF membranes (Millipore, Germany). The PVDF membranes were then incubated with primary antibodies, including anti-SRSF5 (1:1000; Abcam, UK), anti-HOXD8 (1:1000; Santa Cruz, USA), anti-TRIM72 (1:2000; Proteintech, USA), anti-PKM2 (1:1000; CST, USA), and anti-GAPDH (1:5000; ABclonal, China) antibodies at 4 °C. Subsequently, the membranes were incubated with Peroxidase AffiniPure Goat Anti-Rabbit/Mouse IgG (H + L) (1:10000; Jackson ImmunoResearch, USA) for 1 h at room temperature. The protein bands were visualized with a SuperPico ECL Chemiluminescence Kit (Vazyme) and imaged using a ChemiDoc XRS + Imaging System (Bio-Rad, USA).
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