Doxorubicin was purchased from Energy Chemical as a 10 mg/bottle lyophilized powder. The Doxorubicin lyophilized powder was diluted in RPMI‐1640. Peripheral blood mononuclear cells (PBMCs) (106/ml) extracted from DLBCL patients with lymphoma cell infiltration in peripheral blood and LY8 cells (106/ml) were treated with 10, 20, and 40 nM Doxorubicin, respectively, every 2 days for 2 h each for a total of six induction times in vitro.
Doxorubicin
Manufactured by Energy Chemical
Sourced in China
Doxorubicin is a laboratory chemical used for research purposes. It is a cytotoxic anthracycline antibiotic. Doxorubicin is commonly used in cancer research studies.
Automatically generated - may contain errors
Lab products found in correlation
Boron trifluoride diethyl etherate, by Maclin Biochemical Technology (1 mentions)
Hydroxyethyl methacrylate, by Maclin Biochemical Technology (1 mentions)
Human umbilical vein endothelial cells (huvec), by American Type Culture Collection (1 mentions)
Mda mb 231 cell, by American Type Culture Collection (1 mentions)
2 protocols using doxorubicin
1
Doxorubicin Cytotoxicity on DLBCL Cells
2
Synthesis and Evaluation of Targeted Doxorubicin Delivery System
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Hydroxyethyl methacrylate, TEA and boron trifluoride diethyl etherate were purchased from MACKLIN (Shanghai, China). Doxorubicin and beta-D-galactose pentaacetate were obtained from Energy Chemical (Shanghai, China). (Hydroxymethyl) phenylboronic acid pinacol ester, 2,2′-azobis(2-methylpropionitrile) (AIBN, 99%), methacryloyl chloride and 2-(dodecylthiocarbonothioylthio)-2-methylpropionic acid were purchased from Aladdin (Shanghai, China). Dialysis bag MD34 (3500 Da) was obtained from Solarbio (Beijing, China). DiR were purchase from MeilunBio (Suzhou, China). MTT solution, 2% phosphotungstic acid and 4′,6-diamidino- 2-phenylindole (DAPI) were obtained from Solaibio (Shanghai, China).
Female mouse 4T1 cells and human breast cancer MDA-MB-231 cells were obtained from American Type Culture Collection (ATCC, USA) and cultured in complete RMPI 1640 medium with 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin. HUVEC were obtained from ATCC and maintained in HUVEC special culture medium (Procell, Wuhan, China) supplemented with 10% FBS and 1% penicillin/streptomycin. The cells were passaged by mechanical dissociation every two days and further cultured in a humidified incubator with 5% CO2 at 37°C.
Female mouse 4T1 cells and human breast cancer MDA-MB-231 cells were obtained from American Type Culture Collection (ATCC, USA) and cultured in complete RMPI 1640 medium with 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin. HUVEC were obtained from ATCC and maintained in HUVEC special culture medium (Procell, Wuhan, China) supplemented with 10% FBS and 1% penicillin/streptomycin. The cells were passaged by mechanical dissociation every two days and further cultured in a humidified incubator with 5% CO2 at 37°C.
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