The c-MYC gene was visualized by using a blue-staining system (ultraView silver in situ hybridization [SISH] dinitrophenol [DNP] detection kit and c-MYC DNP probe, Ventana Medical Systems, Tucson, AZ, USA). The centromere of chromosome 8 (CEP8) was visualized by using a red-staining system (ultraView red ISH digoxigenin [DIG] detection kit and chromosome 8 DIG probe, Ventana Medical Systems). Positive signals were visualized at 60 × magnification and counted in 50 non-overlapping tumor cell nuclei for each case (Fig 1 ) [17 (link)]. Small and large clusters were scored as 6 and 12 signals, respectively.
C myc dnp probe
Manufactured by Roche
Sourced in United States
The C-MYC DNP probe is a laboratory equipment product designed for use in molecular biology applications. It is a nucleic acid probe that specifically binds to the c-MYC gene sequence, which is a commonly studied oncogene. The probe is labeled with dinitrophenyl (DNP) for detection purposes. The core function of this product is to facilitate the identification and analysis of the c-MYC gene in samples.
Automatically generated - may contain errors
2 protocols using c myc dnp probe
1
Visualizing c-MYC and Chromosome 8 in Tumors
2
Visualizing c-MYC Gene and Chromosome 8 in Tumor Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The c-MYC gene was visualized by using a blue-staining system (ultraView silver in situ hybridization [SISH] dinitrophenol [DNP] detection kit and c-MYC DNP probe, Ventana Medical Systems, Tucson, AZ, USA). The centromere of chromosome 8 (CEP8) was visualized by using a red-staining system (ultraView red ISH digoxigenin [DIG] detection kit and chromosome 8 DIG probe, Ventana Medical Systems). Positive signals were visualized at 60× magnification and counted in 50 non-overlapping tumor cell nuclei for each case. Small and large clusters were scored as 6 and 12 signals, respectively. A c-MYC GCN gain ≥ 4 copies/cell was observed to be the most predictive cut-off point for patient prognosis in a previous study5 (link).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!