L 5 3h proline

Alkaline phosphatase-labeled anti-mouse IgG and anti-rabbit IgG antibodies, bacterial collagenase, 3-(4,5-di-methylthiazole-2-yl)-2,5-diphenyltetrazolium bromide (MTT), Fast BCIP/NBT reagent, l-glycyl-proline, l-proline, monoclonal (mouse) anti-IGF-IR, anti-pERK1/2 and polyclonal (rabbit) anti-β-actin antibodies, sodium pyruvate, and oxythiamine, were provided by Sigma Corp., USA., as were most other chemicals and buffers used. Dulbecco’s minimal essential medium (DMEM) and fetal bovine serum (FBS) used in cell culture were products of Gibco, USA. Glutamine, penicillin, and streptomycin were obtained from Quality Biological Inc., USA. Nitrocellulose membrane (0.2 μm), sodium dodecyl sulphate (SDS), polyacrylamide, molecular weight standards, and Coomassie Brilliant Blue R-250 were received from Bio-Rad Laboratories, USA. L-5[³H] proline (28 Ci/mmol) was purchased from Amersham, UK. Monoclonal (mouse) anti-β₁ and polyclonal (rabbit) anti-α₂-integrin and anti-NFκB p65 antibodies were the products of Santa Cruz Biotechnology Inc., USA. Polyclonal (rabbit) anti-ERK1/2 and monoclonal (rabbit) anti-pAkt antibodies were the products of Cell Signaling Inc., USA. Polyclonal anti-human prolidase antibody was donated by Dr. James Phang (NCI-Frederick Cancer Research and Development Center, Frederick, MD, USA). 3-mercaptopicolinic acid (3-MPA) was purchased from Applichem GmbH, Germany.

MP, PP, radioimmunoprecipitation assay (RIPA) buffer, [3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide] (MTT), sodium dodecyl sulfate (SDS), dimethyl sulfoxide (DMSO), gelatin, bovine serum albumin (BSA), acrylamide and N,N’-Methylenebisacrylamide, bacterial collagenase, and protease inhibitor cocktail (P8340), and N-ethylmaleimide (NEM) were provided by Sigma-Aldrich Corp. (St. Louis, MO, USA). Phosphatase inhibitor cocktail (524625-1SET) was purchased from Merck Millipore Ltd. (Carrigtwohill, County Cork, Ireland). RA was a product of BIOKOM (Warsaw, Poland). Dulbecco’s minimal essential medium (DMEM), phosphate-buffered saline (PBS), and fetal bovine serum (FBS) used in the cell culture were purchased from Gibco (Thermo Fisher Scientific, Waltham, MA, USA). Penicillin, streptomycin, and glutamine were obtained from Quality Biologicals Inc. (Gaithersburg, MD, USA). [³H]thymidine (6.7 Ci/mmol) and L-5[³H] proline (28 Ci/mmol) were purchased from Amersham Biosciences (Buckinghamshire, UK).