Rosa26tomato

The transgenic mouse strains MIP-CreER^T2, MIP-rtTA, PPG-rtTA[26] , and TRE-Nkx6.1 were generated and recently characterized by our laboratory. The transgene constructs were generated by subcloning the coding DNA sequence (CDS) into a plasmid containing the promoter: MIP-CreER^T2, the 1983-bp CreER^T2 CDS following a 8.3-kb mouse insulin 1 promoter; MIP-rtTA, the 747-bp rtTA.M2 CDS following a 8.3-kb mouse insulin 1 promoter; PPG-rtTA, the 747-bp rtTA.M2 CDS following a 1.7-kb mouse preproglucagon promoter; TRE-Nkx6.1, the 1098-bp golden hamster Nkx6.1 CDS following a 0.3-kb TRE-tight promoter. The PANIC-ATTAC transgenic mouse was generated by our laboratory as previously described [22] (link). The mouse strains TRE-Cre (#006234), RIP-rtTA (#008250), Rosa26-LacZ (Rosa26-loxP-STOP-loxP-LacZ, #003474), Rosa26-Tomato (Rosa26-loxP-STOP-loxP-tdTomato Ai9, #007909), and Ptf1a-rtTA (#018070) were purchased from the Jackson Laboratory. All mice were bred in the C57BL/6 genetic background. Mice were fed on regular (LabDiet #5058), high-fat (60%, Research #D12492), or doxycycline chow diet (600 mg/kg, Bio-Serv #S4107). Mice were maintained in 12-h dark/light cycles, with ad libitum access to diet and water. All protocols for mouse use and euthanasia were reviewed and approved by the Institutional Animal Care and Use Committee of the University of Texas Southwestern Medical Center.

Transgenic mice used in this study were Bmi1^CreERT/+, Bmi1^GFP/+, Rosa26^mT.mG/+, Rosa26^Tomato/+ (All from The Jackson Laboratory), Gli1^CreERT/+ [10 (link)], G6PD^Tg [34 (link)] and Sod3^−/− [35 (link)], and all were on the C57BL/6 background. Tamoxifen (Tx; Sigma, T5648) was dissolved in corn oil (Sigma, C8267) and intraperitoneally (i.p.) injected (103 μg/g body weight). Experiments were carried out in male and female mice as recommended by the US National Institutes of Health since preliminary analysis revealed no differences between males and females [36 (link)]. 7-8-week-old mice were used as adult mice unless otherwise indicated in the text. Animal studies were approved by the CNB-CSIC ethics committee and by the Division of Animal Protection of the Comunidad de Madrid (PA 56/11, PROEX 048/16). All animal procedures conformed to EU Directive 2010/63EU and Recommendation 2007/526/EC regarding the protection of animals used for experimental and other scientific purposes, enforced in Spanish law under Real Decreto 1201/2005.