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Alexa fluor 488 dye conjugated secondary antibody

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Alexa Fluor 488 dye-conjugated secondary antibody is a fluorescent labeling reagent used in immunoassays and microscopy applications. The dye is covalently attached to the secondary antibody, which binds to the primary antibody targeting the protein of interest. This allows for the detection and visualization of the target protein.

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Lab products found in correlation

Cy3 labeled ccctaa 3 pna probe, by Panagene (1 mentions) Spider β galactosidase staining kit, by Dojindo Laboratories (1 mentions) Glial fibrillary acidic protein (gfap), by Merck Group (1 mentions) Zen 2009 light edition software, by Zeiss (1 mentions)

Close spelling variants of this product

Alexa Fluor 488 (6806 citations) Alexa 488 (1863 citations) Alexa Fluor-conjugated secondary antibodies (1427 citations) Alexa Fluor 488-conjugated secondary antibody (800 citations) Alexa Fluor secondary antibodies (639 citations) Alexa Fluor 488 secondary antibody (422 citations) Alexa-conjugated secondary antibodies (274 citations) Alexa 488-conjugated secondary antibody (273 citations) Alexa Fluor dyes (206 citations) Alexa Fluor 488 antibody (172 citations)

2 protocols using alexa fluor 488 dye conjugated secondary antibody

1

Telomere Length and Senescence Assays

Cited 2 times
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Telomere length was determined by quantitative telomere in situ hybridization (Q-FISH) [64 (link)] and telomere restriction fragment analysis [65 (link)] using a Cy3-labeled (CCCTAA)3 PNA probe (Panagene) and a Telo-C-Biotin (CCCTAA)3 probe (PNA Innovations), respectively. TIFs were detected by immunofluorescence and telomere-FISH using primary γH2AX antibody (#05-636-AF488, Millipore) and Alexa Fluor 488 dye-conjugated secondary antibody (#R37120, Invitrogen), followed by fixation and telomere-FISH [65 (link)]. TIFs were scored by the co-localization of γ-H2AX and telomere-FISH signal. β-galactosidase activity was carried out using the SPiDER-β-galactosidase staining kit (#SG04-1, Dojindo).
Stock A.J., McDevitt R.A., Puligilla C., Wang Y., Zhang Y., Wang K., Sun C., Becker K.G., Lehrmann E., Wood WH 3.r.d., Gong Y., Aqdas M., Sung M.H., Hoffmann V., Liu C., Gorospe M., Harrington L., Ferrucci L, & Liu Y. (2022). Aberrant expression and localization of the RAP1 shelterin protein contribute to age-related phenotypes. PLOS Genetics, 18(11), e1010506.
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2

Immunolabeling of CNS Cell Types

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For immunolabeling of CNS cell-type-specific markers, cells cultured in 4-well glass chamber slides were fixed with 3.7% paraformaldehyde/PBS, permeabilized using 0.5% Triton X-100, immunolabeled and cell nuclei stained with DAPI. Primary antibodies were directed to GFAP (Millipore; Billerica, MA, USA; catalog AB5804), Iba1 (Wako; Richmond, VA, USA; catalog 019-19741) and MAP2 (Abcam; Cambridge, MA, USA; catalog ab32454), all used at a 1:200 dilution. Primary antibodies were visualized with an Alexa Fluor® 488 dye-conjugated secondary antibody (Invitrogen; catalog A-11034) used at a 1:500 dilution. Samples were imaged using a Zeiss LSM 700 laser scanning confocal microscope. Images were collected with ZEN 2009 Light Edition software (Carl Zeiss) and edited using Adobe Photoshop software (Adobe Systems Incorporated).
Xu R., El-Hage N, & Dever S.M. (2015). Fluorescently-labeled RNA packaging into HIV-1 particles: Direct examination of infectivity across central nervous system cell types. Journal of virological methods, 224, 20-29.
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