Anti ck8

Paraffin-embedded tissues sectioning, H&E staining, and IHC were completed, as described previously (40 (link)). Tissue sections were incubated with the following primary antibodies overnight at 4°C: anti-CK8 (1:200; Developmental Studies Hybridoma Bank, Iowa City, IA, USA); anti-Pax8 (1:200; Proteintech, Chicago, IL, USA); anti-Ki67 and anti-OVGP1 (1:100; Abcam, Cambridge, MA, USA). DAB staining was used to detect protein expression in tissue sections. Stained sections were analyzed by a pathologist (Dr. Jian-Jun Wei, Northwestern University). Slides were imaged on a Nikon Eclipse E600 microscope using the 40× objective.

Cryosections were stained with the following primary antibodies: anti-ACTA2 (1:500; Sigma-Aldrich, F3777 FITC-conjugated or C6198 Cy3-conjugated), anti-vimentin (1:500; Cell Signaling, 5741), anti-calponin (1:500; Abcam, ab46794), anti-collagen type I alpha 2 (1:500; Rockland, 600-401-103-0.1), anti-β-GAL (1:1000; Thermo Fisher Scientific, PA1-21477), anti-EpCAM (1:200; eBioscience, 14-5791-82), anti-GFP/YFP (1:1000; Nacalai Tesque, 0440484), anti-CK5 (1:2000; Covance, PRB-160P), and anti-CK8 (1:500; Developmental Studies Hybridoma Bank, TROMA-1). Secondary antibodies for double labeling were donkey anti-rabbit IgG (H+L) Alexa Fluor 555 (Invitrogen, A-31572), Alexa Fluor 488 (Invitrogen, A-21206) or Alexa Fluor 647 (Invitrogen, A-31573), and goat anti-rat IgG (H+L) Alexa Fluor 555 (Invitrogen, A-21434), all used at 1:1000. Nuclei were counterstained with DAPI.