C57bl 6j trem2em2adiuj j

Manufactured by Jackson ImmunoResearch

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C57BL/6J-Trem2em2Adiuj/J is a mouse strain with a mutation in the Trem2 gene. This mouse strain is commonly used in biomedical research.

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Lab products found in correlation

C57bl 6j, by Jackson ImmunoResearch (2 mentions) Electroforce 3200, by TA Instruments (1 mentions) C57bl 6j bl6, by Jackson ImmunoResearch (1 mentions) B6 fvb tg aldh1l1 egfp rpl10a jd130htz j, by Jackson ImmunoResearch (1 mentions) C57bl 6 tg ubc gfp 30scha j, by Jackson ImmunoResearch (1 mentions)

Close spelling variants of this product

C57BL/6J

4 protocols using c57bl 6j trem2em2adiuj j

Comparative Study of C57BL/6J Mice

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WT C57BL/6J (Jackson Labs #000664) and C57BL/6J-Trem2^em2Adiuj/J (Jackson Labs #027197) mice were procured from Jackson Laboratory and housed at MD Anderson Department of Veterinary Medicine and Surgery under IACUC-approved protocol #00001504-RN02. Mice used for experiments were sex and age matched.

Peshoff M.M., Gupta P., Trivedi R., Oberai S., Chakrapani P., Dang M., Milam N., Maynard M.E., Vaillant B.D., Huse J.T., Wang L., Clise-Dwyer K, & Bhat K.P. (2023). Triggering receptor expressed on myeloid cells 2 (TREM2) regulates phagocytosis in glioblastoma. bioRxiv.

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Trem2 Conditional Knockout in AD Mice

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Trem2^-/- (Trem2 KO) mice were purchased from Jackson Laboratory, USA (C57BL/6J-Trem2^em2Adiuj/J). Trem2^flox/flox mice (C57BL/6J-Trem2^{em2(flox)Smoc}, Shanghai Model Organisms Center Inc., China) were mated with Ctsk-Cre knock-in mice (C57BL/6J-Ctsk^{em1(2A-Cre-Wpre-pA)Smoc}, Shanghai Model Organisms Center) in which the Cre recombinase gene was specifically expressed in osteoclasts. Ctsk-Cre^+/−, Trem2^flox/flox (Trem2 cKO) and Ctsk-Cre^−/−, Trem2^flox/flox (normal littermates) mice were generated by mating Ctsk-Cre^−/−, Trem2^flox/flox male mice with Ctsk-Cre^+/−, Trem2^flox/+ female mice. Heterozygous Tg2576 mice (APP^+/-) overexpressing the human Swedish APP mutation (B6; SJL-Tg(APPSWE)2576Kha, Taconic Biosciences, Germany) on a mixed C57BL/6×SJL background were crossed with Ctsk-Cre^+/-, Trem2^flox/flox mice to generate three groups of mice: APP^-/-, Ctsk-Cre^-/-, Trem2^flox/flox (normal littermates); APP^+/-, Ctsk-Cre^-/-, Trem2^flox/flox (Tg2576); APP^+/-, Ctsk-Cre^+/-, Trem2^flox/flox (Tg2576×Trem2 cKO). In the current study, 2-month-old male mice were used for animal experiments with at least three biological repeats in each group. The mice were maintained in accordance with the National Institutes of Health's Guide for the Care and Use of Laboratory Animals, and the study protocols were approved by The Animal Care and Use Committee of Tongji University.

Weng Y., Wang H., Li L., Feng Y., Xu S, & Wang Z. (2020). Trem2 mediated Syk-dependent ROS amplification is essential for osteoclastogenesis in periodontitis microenvironment. Redox Biology, 40, 101849.

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ACL Injury Model in Mice

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10-week-old male C57Bl/6J (BL6) (Jackson Laboratory Bar Harbor, ME, USA; Stock No: 000664) or Trem2^-/- mice (C57BL/6J-Trem2^em2Adiuj/J; Jackson Laboratory Bar Harbor, ME, USA; Stock No: 027197) were subjected to an ACL injury using a non-invasive single dynamic tibial compressive overload model previously described by Christiansen et al. (21 (link)). Briefly, the mouse knee was placed between two vertically aligned plates positioned using an electromagnetic material testing system (ElectroForce 3200, TA Instruments, New Castle, DE, USA) and a compressive force was applied (21 (link)). ACL rupture occurred after a total compressive force, between 12N-18N, was administered. For pain relief, buprenorphine (0.01 mg/kg) and sterile saline were administered intraperitoneally immediately post-injury. All animal experiments were approved by the Lawrence Livermore National Laboratory and University of California, Davis Institutional Animal Care and Use Committee and conformed to the Guide for the care and use of laboratory animals.

Sebastian A., Hum N.R., McCool J.L., Wilson S.P., Murugesh D.K., Martin K.A., Rios-Arce N.D., Amiri B., Christiansen B.A, & Loots G.G. (2022). Single-cell RNA-Seq reveals changes in immune landscape in post-traumatic osteoarthritis. Frontiers in Immunology, 13, 938075.

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Transgenic Mouse Strains for Alzheimer's Research

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Wild-type C57BL/6J, B6;FVB-Tg(Aldh1l1-EGFP/Rpl10a)JD130Htz/J, C57BL/6J-Trem2em2Adiuj/J, and C57BL/6-Tg(UBC-GFP)30Scha/J mice were purchased from The Jackson Laboratory. 5xFAD mice^{1 (link)} were purchased from the Jackson Laboratory (MMRRC) and backcrossed onto the C57BL/6J background more than 10 generations before being crossed with other strains. Il3^−/− mice on the C57BL/6J background were bred in-house^{2 (link),3} and crossed with 5xFAD mice. For RNAseq studies, Trem2^−/− mice^{4 (link)} on the C57BL/6J background were generated at Washington University School of Medicine, bred in-house, and crossed with 5xFAD mice. Age- and sex-matched animals were used. If sex of the animals is not specifically indicated, groups were sex balanced. Where appropriate, animals were randomly assigned to interventions. All mice were group housed under standard conditions with free access to food and water. All animal protocols were approved by the Animal Review Committee at the Massachusetts General Hospital (protocol no. 2011N000035 and 2015N000044) and were in compliance with relevant ethical regulations.

McAlpine C.S., Park J., Griciuc A., Kim E., Choi S.H., Iwamoto Y., Kiss M.G., Christie K.A., Vinegoni C., Poller W.C., Mindur J.E., Chan C.T., He S., Janssen H., Wong L.P., Downey J., Singh S., Anzai A., Kahles F., Jorfi M., Feruglio P.F., Sadreyev R.I., Weissleder R., Kleinstiver B.P., Nahrendorf M., Tanzi R.E, & Swirski F.K. (2021). Astrocyte-derived interleukin-3 reprograms microglia and limits Alzheimer’s disease. Nature, 595(7869), 701-706.

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