The whole genome of the M. purpureus CSU-M183 strain was sequenced using SMRT sequencing technology of PacBioRS II, and the sequencing quality was improved using Illumina NGS platform. The sequencing library was constructed using the TruSeqTM Nano DNA LT Sample Prep Kit–Set A (Illumina, USA) and amplified using the TruSeq PE Cluster Kit (Illumina, USA).
The quality of the assembled genome and annotated geneset were assessed first using the Benchmarking Universal Single-Copy Orthologs (version 3.1.0; BUSCO) with the fungi_odb9 dataset [33 (link)].
For raw data polymerase reads after PacBioRS II sequencing, subreads were obtained by removing the low-quality or unknown reads, adapters and duplications. The filtered reads were assembled de novo using the Hierarchical Genome Assembly Process (HGAP) algorithm version 2.0 [34 (link)].
For genome assembly, the default parameters of HGAP2 were used (Minimum Subread Length = 500, Minimum Polymerase Read Quality = 0.80, Minimum Polymerase Read Length = 100, Overlapper Error Rate = 0.06, Overlapper Min Length = 40) with input genome size as 30 Mb.
The quality of the assembled genome and annotated geneset were assessed first using the Benchmarking Universal Single-Copy Orthologs (version 3.1.0; BUSCO) with the fungi_odb9 dataset [33 (link)].
For raw data polymerase reads after PacBioRS II sequencing, subreads were obtained by removing the low-quality or unknown reads, adapters and duplications. The filtered reads were assembled de novo using the Hierarchical Genome Assembly Process (HGAP) algorithm version 2.0 [34 (link)].
For genome assembly, the default parameters of HGAP2 were used (Minimum Subread Length = 500, Minimum Polymerase Read Quality = 0.80, Minimum Polymerase Read Length = 100, Overlapper Error Rate = 0.06, Overlapper Min Length = 40) with input genome size as 30 Mb.