DNA damage was assessed using the OxiSelect DNA Double Strand Break (DSB) staining kit (Cell BioLabs, San Diego, CA). N9 microglia were plated in black-walled 96-well plates at a density of 10,000 cells/well. The following day the cells were exposed to 0, 50, or 100 mJ/cm2∙min for 6 hours using the 96-LED device with half of the wells for each light dosage receiving etoposide (100 uM; Cell BioLabs) during the last 1 hour to serve as positive controls. Cells were then immediately fixed and stained as per kit instructions. Fluorescent staining was visualized on a Nikon TS-100 microscope equipped with a B-2E/C filter cube (Nikon, Melville, NY) with illumination provided by a 120 W light source (XCite 120 lamp; Lumen Dynamics, Mississauga, ON, Canada). Images were captured using a Nikon DS-L1 camera system; illumination intensity and exposure time were kept constant across all images.
Xcite 120 lamp
Manufactured by Excelitas
Sourced in Canada, Japan
The XCite 120 lamp is a high-performance illumination source designed for a wide range of laboratory applications. It features a powerful 120W metal halide lamp that provides bright, stable, and uniform illumination. The lamp is suitable for use with various microscopy and imaging techniques.
Automatically generated - may contain errors
2 protocols using xcite 120 lamp
1
Quantifying DNA Damage in Microglia
2
Fluorescence Microscopy Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The microscopic studies were performed using an inverted Olympus IX 71S8F-3 fluorescence microscope (Olympus, Tokyo, Japan) equipped with a mercury arc lamp, X-cite 120 Lamp (120 W; Lumen Dynamics, Mississauga, Canada) for illumination. In this experiment, an excitation filter of 545–580 nm and an emission filter of 610 nm were used. The images were obtained using a Camera Olympus DP73 (Olympus) and processed by Stream Basic1.7 software (Olympus Soft Imaging Solutions GmbH, Münster, Germany) using a resolution of 1,600×1,200 pixels.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!