Vacutainer plus plastic serum and sst tubes

Manufactured by BD

The BD Vacutainer Plus Plastic Serum and SST Tubes are laboratory blood collection tubes used for the collection, transportation, and processing of blood samples. These tubes are designed to separate serum or plasma from the cellular components of the blood sample.

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Lab products found in correlation

Mirneasy mini kit, by Qiagen (1 mentions)

Close spelling variants of this product

BD Vacutainer Plus Plastic Serum Serum vacutainer tubes BD Plus-Plastic tubes BD Vacutainer SST SST tubes Plastic tubes Vacutainer tubes Serum vacutainers BD Vacutainer Plus Serum tube

4 protocols using vacutainer plus plastic serum and sst tubes

Serum Sampling and Storage Protocol

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Blood samples were collected in BD Vacutainer Plus Plastic Serum and SST Tubes, which are coated with silicone and micronized silica particles to accelerate clotting. Samples were then centrifuged at 1500g for 15 min at room temperature, and supernatants were aliquoted as serum samples and immediately stored at –70 ^oC until measurement.

Yanagisawa S., Papaioannou A.I., Papaporfyriou A., Baker J.R., Vuppusetty C., Loukides S., Barnes P.J, & Ito K. (2017). Decreased Serum Sirtuin-1 in COPD. Chest, 152(2), 343-352.

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Blood Sample Preparation for Biomarker Analysis

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Blood samples were collected in BD Vacutainer Plus Plastic Serum and SST Tubes, which are coated with silicone and micronized silica particles to accelerate clotting. Samples were then centrifuged at 2500 g for 15 min at room temperature, and serum supernatants were aliquoted and immediately stored at −80 °C until measurement.

Tsilogianni Z., Baker J.R., Papaporfyriou A., Papaioannou A.I., Papathanasiou E., Koulouris N.G., Daly L., Ito K., Hillas G., Papiris S., Bakakos P, & Loukides S. (2020). Sirtuin 1: Endocan and Sestrin 2 in Different Biological Samples in Patients with Asthma. Does Severity Make the Difference?. Journal of Clinical Medicine, 9(2), 473.

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Serum RNA Isolation Protocol

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Fasting serum sample (from 5 ml of blood) was obtained by standard venepuncture using VacutainerPlus Plastic Serum and SST Tubes (Becton-Dickinson, Franklin, lakes, NJ). The separation of the serum was performed by centrifugation at 4,000 rpm for 10 min, followed by 12000 rpm for 15 min to completely remove cell debris. Total RNA was isolated from 0.25 ml of serum using Qiagen miRNeasy Mini kit (Qiagen, Valencia, CA) for RNA collection and purification according to the manufacturer's protocol. Total RNA was controlled using Nanodrop 2000 UV spectrophotometer and stored at -80°C until use.

Prabu P., Rome S., Sathishkumar C., Aravind S., Mahalingam B., Shanthirani C.S., Gastebois C., Villard A., Mohan V, & Balasubramanyam M. (2015). Circulating MiRNAs of ‘Asian Indian Phenotype’ Identified in Subjects with Impaired Glucose Tolerance and Patients with Type 2 Diabetes. PLoS ONE, 10(5), e0128372.

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Obese Pre-Diabetic Asian Indian Cohort

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Data and samples from sixty Asian Indian obese pre-diabetic patients were selected from one of the intervention studies of the Madras Diabetes Research Foundation (Chennai, India). They belong to the D-CLIP study (Diabetes Community Lifestyle Improvement Program, Clinicaltrial.gov NCT01283308) [16 (link), 20 (link)] designed to test the effectiveness of stepwise diabetes program [16 (link)]. None of them were on medications at the start of the program. At baseline state, questionnaires permitted to measure habits, quality of life and food frequency of the participants. Anthropometric measurements including height, weight and waist circumstance were obtained using standardized techniques [5 (link)]. For this study, fasting serum samples from 5ml of blood were obtained at basal state, 4 months and one year by standard venepuncture using Vacutainer Plus Plastic Serum and SST Tubes (Becton-Dickinson, Franklin, lakes, NJ). Metabolic parameters were measured as previously detailed [5 (link)]. IL-6, adiponectin, MCP, TNF-alpha, Irisin and BDNF were quantified with ELISA tests [20 (link)]. At baseline, metabolic parameters did not differ significantly between the 2 groups of pre-diabetic individuals, except for the age (S1–S3 Tables).

Paramasivam P., Meugnier E., Gokulakrishnan K., Ranjini H., Staimez L.R., Weber M.B., Narayan K.M., Vidal H., Tandon N., Prabhakaran D., Mohan A.R., Mohan V., Rome S, & Balasubramanyam M. (2022). Blood-derived miRNA levels are not correlated with metabolic or anthropometric parameters in obese pre-diabetic subjects but with systemic inflammation. PLoS ONE, 17(2), e0263479.

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