Three CRC tissues, the matched paracancerous normal intestinal mucosal tissues, and CRC cells derived from a primary tumor (SW480) and lymph node metastasis (SW620) from the same patient were used for the lncRNA expression profiling analysis. In brief, we first extracted the total RNA CRC tissues and cells. Then, the RNA was synthesized into double‐stranded cDNA. LncRNA Expression Microarray (Arraystar) was used to label, purify, and hybridize the cDNA. An Agilent Microarray Scanner (Agilent p/n G2565BA) was used to scan slides after hybridization and washing. Finally, we extracted the data using Agilent Feature Extraction Software. We use Student's t test to calculate a p value. The differentially expressed genes threshold was set as a p value ≤0.05 and a fold change ≥2.0. The microarray data in this study have been uploaded to the National Center for Biotechnology Information (NCBI) Gene Expression Omnibus (GEO). The GEO Series accession number is GSE184903.
Lncrna expression microarray
Manufactured by Arraystar
Sourced in United States
The LncRNA Expression Microarray is a laboratory instrument designed for the detection and analysis of long non-coding RNA (lncRNA) expression levels. It provides a comprehensive platform for researchers to study the role of lncRNAs in various biological processes and diseases.
Automatically generated - may contain errors
Lab products found in correlation
Trizol reagent, by Thermo Fisher Scientific (2 mentions)
Microarray scanner, by Agilent Technologies (1 mentions)
Agilent feature extraction software, by Agilent Technologies (1 mentions)
Ethylene diamine tetraacetic acid (edta), by Thermo Fisher Scientific (1 mentions)
Genespring gx v12, by Agilent Technologies (1 mentions)
Rneasy mini kit, by Qiagen (1 mentions)
Trizol kit, by Thermo Fisher Scientific (1 mentions)
Superscript double stranded cdna synthesis kit, by Thermo Fisher Scientific (1 mentions)
Axon genepix 4000b scanner, by Molecular Devices (1 mentions)
Nimblescan software, by Roche (1 mentions)
4 protocols using lncrna expression microarray
1
lncRNA Expression Profiling in Colorectal Cancer
2
LncRNA Expression Microarray Analysis of ALD-Treated Mast Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Performed as previously described by Zhang et al (29 (link)), with 10−6 M ALD-treated MCs being used as the experimental cells. An equal amount of solvent (DMEM+10% FCS) was added to the solvent control group. Cells were cultured in 6-well plates for 24 h. MCs were washed with PBS three times, then 200 µl EDTA (Gibco; Thermo Fisher Scientific, Inc.) was added to each well followed by 750 µl TRIzol® reagent (Invitrogen; Thermo Fisher Scientific, Inc.). The cells were stored at −80°C and sent to the LncRNA Expression Microarray (Arraystar, Rockville, MD, USA).
3
Profiling Differential lncRNA Expression in NPC
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Total RNA was extracted from three NPC tissues and three normal nasopharyngeal epithelial tissues using TRIzol Reagent (Invitrogen) and purified by an RNeasy Mini Kit (Qiagen, Valencia, CA). cDNA was synthesized, labeled, and hybridized to the LncRNA Expression Microarray (Arraystar, Rockville, MD). After washing, slides were analyzed on the lncRNAs microarray. Differentially expressed lncRNAs were identified with thresholds of P < 0.05 and fold change ≥2.0 using GeneSpring GX v12.1 software package (Agilent Technologies, Santa Clara, CA, USA).
4
Profiling lncRNA Expression in Rat Kidney I/R
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Total RNA of kidney tissues in rats from the sham and I/R groups was extracted by a TRIzol kit (Invitrogen Inc., Carlsbad, CA, USA). Double strand cDNA was synthesized by SuperScript Doublestranded cDNA synthesis kit (Invitrogen), and labeled and hybridized to an lncRNA expression microarray (12 × 135K, Arraystar Inc., Rockville, MD, USA). After hybridization and washing, processed slides were scanned by an Axon GenePix 4000B scanner (Molecular Devices Inc., Sunnyvale, CA, USA). Raw data were extracted as pair les using NimbleScan software (Version 2.5; Roche). The threshold for up-and downregulated genes was set as fold change > = 1.5 and p value < = 0.05. All the above works were completed by Shanghai Sensichip Hightech Co., Ltd. (Shanghai, China). Hierarchical cluster analysis was done by Shanghai Novel Bioinformatics Company (Shanghai, China).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!