Genechip mogene 2.0 st array

RNA was extracted from control or Hnf6/Oc2 double-mutant spinal cords. The tissue was manually dissociated in Tripur isolation reagent (Roche, 11 667 165 001). After dissociation, chloroform (Merck Millipore, 1 02445 1000) was added to the sample, incubated at room temperature for 10 min and centrifugated for 10 min at 4°C. The aqueous phase was collected and the RNA was precipitated with isopropanol (VWR, 20880.310) and centrifugated for 15 min at 4°C. The pellet was washed in ethanol (Biosolve, 06250502) and centrifugated for 10 min at 4°C. The dried pellet was resuspended in RNAse free water. The integrity of the RNA was assessed using an Agilent RNA 6000 Nano assay procedure. For microarray analyzes, the RNA was converted in single-strand cDNA, labeled using the GeneChip^® WT PLUS Reagent Kit (Affymetrix) and hybridized on the GeneChip^® MoGene 2.0 ST array (Affymetrix, 90 2118) using Affymetrix devices: Genechip^® Fluidics Station 450, Genechip^® Hybridization oven 640, Affymetrix Genechip^® scanner and the Expression Consol software. The analyzes were performed using the R software. Microarray data have been deposited in the GEO repository (accession number: GSE117871).