Cubilin

Manufactured by Santa Cruz Biotechnology

Cubilin is a multi-ligand receptor involved in the reabsorption of various molecules in the proximal renal tubules. It functions as a component of the albumin-binding complex responsible for the endocytic uptake of albumin and other proteins filtered through the glomerulus.

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Lab products found in correlation

Megalin, by Santa Cruz Biotechnology (2 mentions) Ab131487, by Abcam (1 mentions) B 1035, by Vector Laboratories (1 mentions) Villin 1, by Cell Signaling Technology (1 mentions) Sp 2001, by Vector Laboratories (1 mentions) Polycystin 1, by Santa Cruz Biotechnology (1 mentions) Laminin, by Merck Group (1 mentions) B 1325, by Vector Laboratories (1 mentions)

2 protocols using cubilin

Renal Tissue Analysis Using Multimodal Imaging

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(1) Immunohistochemistry (IHC). Renal tissues were immediately fixed in 4% formalin and subsequently embedded in paraffin. The expression of IRS-1 (ab131487, abcam), pSer IRS-1 (AF3272, Affinity), megalin (Santa Cruz, Sc-25470), and cubilin (Santa Cruz, Sc-23644) in renal tubules and interstitium were detected by IHC.
(2) Light Microscope. Renal tissues were fixed in 4% paraformaldehyde and embedded in paraffin. Tissue slices were cut at 4 μm thickness, dewaxed in xylene, rehydrated in decreasing concentrations of ethanol in water and stained by H&E, and then examined by a light microscope.
(3) Transmission Electron Microscope. Renal tissues were immediately placed in fixative (2.5% glutaraldehyde and 1% osmium tetraoxide), dehydrated using graded alcohol and epoxypropane, and then embedded in Epon 812. Ultrathin sections (50 μm±) were cut using an ELICA ULTRACUT-R ultramicrotome and stained with uranyl acetate and lead citrate. The sections were examined using a HITACHI-7500 transmission electron microscope.

Zhang Y., Yang S., Cui X., Yang J., Zheng M., Jia J., Han F., Yang X., Wang J., Guo Z., Chang B, & Chang B. (2019). Hyperinsulinemia Can Cause Kidney Disease in the IGT Stage of OLETF Rats via the INS/IRS-1/PI3-K/Akt Signaling Pathway. Journal of Diabetes Research, 2019, 4709715.

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Comprehensive Kidney Tissue Imaging Protocols

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For immunohistochemistry (IHC), kidney sections were pretreated to quench endogenous peroxidase (3.0% hydrogen peroxide) and endogenous biotin (SP-2001, Vector Labs), and stained with biotinylated LTL (B-1325, Vector Labs) and DBA (B-1035, Vector Labs) following standard IHC protocol. For immunofluorescence staining, mouse kidneys were fixed in 4% PFA followed by incubation in 30% sucrose overnight at 4°C, embedded in OCT compound (Tissue-Tek), and cryosectioned at 10 μm. Frozen sections were permeabilized with 0.1% PBS- Triton X-100 for 10 min and blocked in 5% goat serum for 1 hour. Primary antibodies were incubated overnight at 4°C followed by secondary antibodies incubated at room temperature for 1 hour. Following primary antibodies were used: WT1 (sc-192, Santa Cruz), Polycystin-1 (sc-130554, Santa Cruz), Laminin (L9393, Sigma), GFP (GFP-1020, Aves), Villin-1 (2369, Cell Signaling), JAG1 (sc-8303, Santa Cruz), PAX2 (71-6000, Thermo Fisher), megalin (sc-16478, Santa Cruz), cubilin (sc-20609, Santa Cruz). Tissue sections were mounted in media containing DAPI and imaged by a Zeiss confocal microscope.

Rasouly H.M., Kumar S., Chan S., Pisarek-Horowitz A., Sharma R., Xi Q.J., Nishizaki Y., Higashi Y., Salant D.J., Maas R.L, & Lu W. (2016). Loss of Zeb2 in mesenchyme-derived nephrons causes primary glomerulocystic disease. Kidney international, 90(6), 1262-1273.

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