PCR reactions contained 1× Sigma Taq buffer, 2 mM deoxyribonucleotide triphosphate (dNTP) mix, 2 mM MgCl2, 1 µM forward and reverse primers (Supplemental Table 3), 0.1 µL Sigma Taq DNA polymerase, and 4 µL DNA in a total volume of 20 µL. Reactions were thermocycled for 2 min at 95°C followed by 35 cycles for 30 sec at 95°C, 30 sec at 55°C and 5 min at 72°C, and finally for 5 min at 72°C. Products were resolved on a 0.7% agarose gel.
Sigma taq dna polymerase
Manufactured by Merck Group
Sigma Taq DNA polymerase is a thermostable DNA polymerase enzyme derived from the bacterium Thermus aquaticus. It is used for the amplification of DNA sequences through the Polymerase Chain Reaction (PCR) technique.
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Lab products found in correlation
2 protocols using sigma taq dna polymerase
1
Optimized PCR Amplification Protocol
2
Genotyping of MYH7b Null Mice
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The isolated genomic DNA was analyzed using Sigma Taq DNA Polymerase from Thermus aquaticus (product number D4545). The following primer pairs were used for genotyping (mMYH7b_forward 5′ TGC TCT TAT GTC TGG AGG TGT and mMYH7b_reverse 5′ GGT AGC AGG CTG ATT CTC C or polyA‐reverse 5′ TCC ACT AGT TCT AGA GCG GC). Thermocycling conditions were: 95°C for 15 seconds followed by 33 cycles: 95°C for 15 seconds, 58°C for 15 seconds, 72°C for 30 seconds followed by a final extension at 72°C for 10 minutes. Polymerase chain reaction (PCR) products from each reaction were analyzed by 1% agarose gel. MYH7b null mice were identified by the presence of a band at 218 bp, and wild‐type mice were identified by the presence of a band at 242 bp.
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