Four-week-old male nude mice purchased from HFK BioScience (Beijing, China) were housed under specific pathogen-free (SPF) conditions, and then bred as per Institutional Laboratory Guidelines for Animal Care. BEL-7402-control and BEL-7402-overexpress miR-631 cells (1 × 106) were suspended with 100 μl DMEM and injected subcutaneously into the flanks of nude mice. After 4 weeks, we anatomized the mice and removed the tumors, cut the tumors into small tissues of approximately 1 mm3, then transplanted them into the livers of nude mice (six mice per group) (13 (link)). Some 7 weeks later, liver tissues of the nude mice were dissected and fixed. All animal experiments complied with the ARRIVE (Animal Research: Reporting of In Vivo Experiments) guidelines.
Nude mice
Manufactured by Beijing Huafukang Biotechnology
Sourced in China
Nude mice are laboratory-bred mice that lack a functional immune system, specifically the thymus gland. This genetic mutation results in the mice being devoid of T cells, making them highly susceptible to infections and useful for research purposes where a compromised immune system is required.
Automatically generated - may contain errors
Lab products found in correlation
Balb c mice, by Beijing Huafukang Biotechnology (3 mentions)
Nod scid mice, by Beijing Huafukang Biotechnology (1 mentions)
C57bl 6j mice, by Beijing Huafukang Biotechnology (1 mentions)
Wistar rats, by Beijing Huafukang Biotechnology (1 mentions)
C57bl 6 mice, by Beijing Huafukang Biotechnology (1 mentions)
Dehydrated alcohol, by Thermo Fisher Scientific (1 mentions)
Acetonitrile, by Thermo Fisher Scientific (1 mentions)
Methanol, by Thermo Fisher Scientific (1 mentions)
Oleic acid, by Lipoid (1 mentions)
Iwp 2, by Merck Group (1 mentions)
16 protocols using nude mice
1
Tumor Implantation in Nude Mice
2
Investigating Circulating RNA in Nude Mice
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In this experiment, 20 nude mice (male, 4 weeks old) (both Beijing Huafukang Biotechnology Co., Ltd., Beijing, China) were selected and raised in sterile laminar flow animal rooms (SPF grade). Lentivirus plasmids of short hairpin RNA (shRNA) against circ-10720 (sh-circ-10720) and negative control (sh-NC) were constructed by Genepharma (Shanghai, China). Cells were infected with lentiviral particles of sh-circ-10720 or sh-NC, and then, nude mice were injected with 2 × 106 cells into the subcutaneous area of the right back. The date and time of cell inoculation were recorded, and each nude mouse was marked. After 7 days, the longest diameter (length) and shortest diameter (width) of the tumors were measured with a ruler sterilized by UV radiation. After that, tumor volume was calculated: π/6 × (length × width)2. After 4 weeks, mouse euthanasia was done with CO2, and tumors were weighed [35 (link)].
3
Xenograft Tumor Growth Monitoring
4
Ethical Animal Care Protocols
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Animal care and use were conducted according to the Third Military Medical University (Army Medical University) for the Care and Use of Laboratory Animals. BALB/C mice and nude mice (6–8 weeks old), ranging from 20 to 25 g, were purchased from BEIJING HFK BIOSCIENCE CO.LTD.
5
Establishing HeLa Tumor Model in Nude Mice
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Nude mice weighing 20 g were purchased from HFK Bioscience Co. (Beijing, China). All the animal studies were performed in compliance with guidelines set by the Animal Care Committee at Tongji Medical College. To establish the HeLa tumor model, 1 × 106 cells were inoculated subcutaneously into the right front flanks of male BALB/c Nude mice. Tumor growth was measured using a caliper, and the tumor volume was calculated using the following formula: volume = ((tumor length) × (tumor width)2)/2.
6
Wistar Rat and Nude Mouse Study
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Twelve male Wistar rats weighting 180 ± 20 g and 20 nude mice weighing 20–25 g were obtained from Beijing HFK Bioscience Co. Ltd. (Beijing, China). All animal-use procedures complies with relevant regulations. Rats were housed in accordance with the following conditions: the interval of illumination is 12 hours, the indoor temperature is basically constant, about 25 °C, the relative humidity was 45.0–60.0%. The rats were fasted for 12.0 h but had free access to water prior to administration.
7
Nude Mice Xenograft Model for Tumor Studies
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Four to five‐week‐old female nude mice were purchased from Beijing Huafukang Bioscience (Beijing, China). To construct the xenograft mouse models, 5 × 106 HCT116, C116 and A116 cells were subcutaneously injected into the dorsal area of the right flank of the mice separately. Tumour sizes were monitored every 3 days. After 27 days, mice were sacrificed for subsequent experiments. The in vivo study received approval from the Animal Welfare and Ethics Committee of Army Medical University (No. AMUWEC20173938).
8
Xenograft and Metastasis Assay in Nude Mice
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Nude mice (6 weeks old) were purchased from Beijing Huafukang Bioscience Co. INC and maintained in micro-isolator cages in SPF laboratory animal room. All animals were used in accordance with institute guidelines and the experiments were approved by the Use Committee for Animal Care of the institute. For subcutaneous inoculation, cells re-suspended in PBS at a concentration of 2 × 107 cells/mL were injected into 8-week old Nude mice. The tumors were measured every 3 days after appearance and the tumor volumes were calculated by the formula (length×width)2/2. For metastatic assay, cells with overexpression or knockdown of DTL and corresponding controls were re-suspended in PBS at a concentration of 1 × 107 cells/mL. Cell suspension (0.1 mL) was injected into tail veins of Nude mice. The Nude mice were sacrificed by anesthesia with chloral hydrate.
9
Nude Mice Xenograft Tumor Assay
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Nude mice (6–7 weeks old, n = 10) were purchased from Beijing HFK Bioscience Co. Ltd. (Beijing, China). The animal studies were approved by Tianjin Institute of Urology, Tianjin, China. Subcutaneous tumor growth assays were performed with 22RV1 shSCR (control) and shARHGEF2 stable cell lines (2 × 106 shSCR cells injected to 5 mice separately, 2 × 106 shARHGEF2 cells injected to another 5 mice separately). At the end point, all mice were sacrificed, and the tumors were harvested under standard, institutionally approved processes.
10
Nude Mice Xenograft Tumor Assay
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Nude mice (6–7 weeks old, n = 10) were purchased from Beijing HFK Bioscience Co. Ltd. (Beijing, China). The animal studies were approved by Tianjin Institute of Urology, Tianjin, China. Subcutaneous tumor growth assays were performed with 22RV1 shSCR (control) and shARHGEF2 stable cell lines (2 × 106 shSCR cells injected to 5 mice separately, 2 × 106 shARHGEF2 cells injected to another 5 mice separately). At the end point, all mice were sacrificed, and the tumors were harvested under standard, institutionally approved processes.
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