Linear ion trap (LIT) and triple quadrupole (QQQ) scans were acquired on a triple quadrupole-linear ion trap mass spectrometer (Q-TRAP), API 4500 Q-TRAP UPLC/MS/MS system, equipped with an ESI Turbo Ion-Spray interface, operating in positive and negative ion mode and controlled by Analyst 1.6.3 software (AB Sciex). Instrument tuning and mass calibration were performed with 10 and 100 μmol/L polypropylene glycol solutions in QQQ and LIT modes, respectively. Triple quadrupole scans were acquired as metabolic response modifier experiments with collision gas (nitrogen) set to 5 psi. Declustering potential (DP) and collision energy (CE) for individual multiple reactions monitoring (MRM) transitions were done with further DP and CE optimization. A specific set of MRM transitions were monitored for each period according to the metabolites eluted within this period.
Api 4500 q trap uplc ms ms system
Manufactured by AB Sciex
Sourced in United States
The AB Sciex API 4500 Q TRAP UPLC/MS/MS System is a high-performance liquid chromatography-tandem mass spectrometry (UPLC/MS/MS) system. It is designed for the analysis of a wide range of molecules, providing high sensitivity and selectivity. The system combines the UPLC separation technology with the Q TRAP mass spectrometry platform, which includes a triple quadrupole and a linear ion trap. This combination enables the system to perform both quantitative and qualitative analyses with a high level of precision and accuracy.
Automatically generated - may contain errors
10 protocols using api 4500 q trap uplc ms ms system
1
Quantitative mass spectrometry analysis
2
QTRAP Mass Spectrometry Protocol for Metabolite Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Linear ion trap (LIT) and triple quadrupole (QQQ) scans were acquired on a triple quadrupole-linear ion trap mass spectrometer (QTRAP, API 4500 QTRAP UPLC-MS/MS system) equipped with an ESI turbo ion-spray interface, operating in positive and negative ion modes and controlled by the Analyst 1.6.3 software (AB Sciex, Framingham, MA, USA). The ESI source operation parameters were as follows: ion source, turbo spray; source temperature 550 ℃; and ion spray voltage (IS) 5500 V (positive ion mode)/-4500 V (negative ion mode). In addition, the ion source gas I (GSI), gas II (GSII) and curtain gas (CUR) were set at 55.0 psi, 60.0 psi and 25.0 psi, respectively, and the collision-activated dissociation (CAD) was set at high. Instrument tuning and mass calibration were performed using 10 and 100 µmol/L polpolypropylene glycol solutions in QQQ and LIT modes, respectively. QQQ scans were acquired as (MRM) experiments with a collision gas (nitrogen) pressure of 5 psi. Declustering potential (DP) and collision energy (CE) were performed for individual MRM transitions with further DP and CE optimization. A specific set of MRM transitions were monitored for each period according to the metabolites eluted within this period [20 (link)].
3
Quantitative Mass Spectrometry Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Linear ion trap (LIT) and triple quadrupole (QQQ) scans were acquired on a triple quadrupole-linear ion trap mass spectrometer (Q TRAP), API 4500 Q TRAP UPLC/MS/MS System, equipped with an ESI Turbo Ion-Spray interface, operating in positive and negative ion mode and controlled by Analyst 1.6.3 software (AB Sciex). The main parameters were as follows: the temperature of electrospray ionization was set to 550 °C; the voltages of the ion sprays were 5500 V and −4500 V in positive and negative ion modes, respectively; the curtain gas (CUR) was 30 psi, and the collision-activated dissociation (CAD) was high. Each ion pair was scanned under the conditions of the optimum declustering potential (DP) and collision energy (CE) in the QQQ system [48 (link)]. Instrument tuning and mass calibration were performed with 10 and 100 μ mol/L polypropylene glycol solutions in QQQ and LIT modes, respectively. QQQ scans were acquired as MRM experiments with collision gas (nitrogen) set to 5 psi. DP and CE for individual MRM transitions were performed with further DP and CE optimization. A specific set of MRM transitions was monitored for each period according to the metabolites eluted within this period.
4
Metabolite Profiling by Q TRAP LC-MS/MS
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
LIT and triple quadrupole (QQQ) scans were acquired on a triple quadrupole-linear ion trap mass spectrometer (Q TRAP), API 4500 Q TRAP UPLC/MS/MS System, equipped with an ESI Turbo Ion-Spray interface, operating in positive and negative ion mode and controlled by Analyst 1.6.3 software (AB Sciex). The ESI source operation parameters were as follows: an ion source, turbo spray; source temperature 550 °C; ion spray voltage (IS) 5500 V (positive ion mode)/− 4500 V (negative ion mode); ion source gas I (GSI), gas II (GSII), curtain gas (CUR) were set at 50, 60, and 30.0 psi, respectively; the collision gas (CAD) was high. Instrument tuning and mass calibration were performed with 10 and 100 μmol L− 1 polypropylene glycol solutions in QQQ and LIT modes, respectively. QQQ scans were acquired as MRM (multiple reaction monitoring) experiments with collision gas (nitrogen) set at 5 psi. DP and CE for individual MRM transitions were done with further optimization of the two parameters. Specific sets of MRM transitions were monitored for each period according to the metabolites eluted within the period.
5
Optimized ESI-Q TRAP-MS/MS Settings
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The ESI-Q TRAP-MS/MS settings were as reported earlier [121 ]. Briefly, LIT and triple quadrupole (QQQ) scans were acquired on a triple quadrupole-linear ion trap mass spectrometer (Q TRAP), API 4500 Q TRAP UPLC/MS/MS System, equipped with an ESI Turbo Ion-Spray interface, operating in positive and negative ion mode and controlled by Analyst 1.6.3 software (AB Sciex). The ESI source operation parameters were as follows: ion source, turbo spray; source temperature 550 ℃; ion spray voltage (IS) 5500 V (positive ion mode)/ -4500 V (negative ion mode); ion source gas I (GSI), gas II (GSII), curtain gas (CUR) were set at 50, 60, and 30.0 psi, respectively; the collision gas (CAD) was high. Instrument tuning and mass calibration were performed with 10 and 100 μmol/L polypropylene glycol solutions in QQQ and LIT modes, respectively. QQQ scans were acquired as MRM experiments with collision gas (nitrogen) set to 5 psi. Declustering potential (DP) and collision energy (CE) for individual MRM transitions was done with further DP and CE optimization. A specific set of MRM transitions were monitored for each period according to the metabolites eluted within this period.
6
Targeted Metabolite Profiling by UHPLC-MS/MS
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
LIT and triple quadrupole (QQQ) scans were acquired on a triple quadrupole-linear ion trap mass spectrometer (Q TRAP), API 4500 Q TRAP UPLC/MS/MS System, equipped with an ESI Turbo Ion-Spray interface, operating in positive and negative ion mode and controlled by Analyst 1.6.3 software (AB Sciex). The ESI source operation parameters were as follows: ion source, turbo spray; source temperature 550℃; ion spray voltage (IS) 5500 V (positive ion mode)/-4500 V (negative ion mode); ion source gas I (GSI), gas II(GSII), curtain gas (CUR) were set at 50, 60, and 30.0 psi, respectively; the collision gas(CAD) was high. Instrument tuning and mass calibration were performed with 10 and 100 µmol/L polypropylene glycol solutions in QQQ and LIT modes, respectively. QQQ scans were acquired as MRM experiments with collision gas (nitrogen) set to 5 psi. DP and CE for individual MRM transitions was done with further DP and CE optimization. A specific set of MRM transitions were monitored for each period according to the metabolites eluted within this period.
7
Quantitative Analysis of Metabolites
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
LIT and triple quadrupole (QQQ) scans were acquired on a triple quadrupole-linear ion trap mass spectrometer (Q TRAP), API 4500 Q TRAP UPLC/MS/MS system, equipped with an ESI turbo ion spray interface, operating in positive and negative ion mode and controlled by Analyst 1.6.3 software (AB Sciex). The ESI source operation parameters were as follows: ion source, turbo spray; source temperature 550 °C; ion spray voltage (IS) 5500 V (positive ion mode)/-4500 V (negative ion mode); ion source gas I (GSI), gas II(GSII), and curtain gas (CUR) set at 50, 60, and 30.0 psi, respectively; and the collision gas (CAD) was high. Instrument tuning and mass calibration were performed with 10 and 100 μmol/L polypropylene glycol solutions in QQQ and LIT modes, respectively. QQQ scans were acquired as MRM experiments with collision gas (nitrogen) set to 5 psi. DP and CE for individual MRM transitions were done with further DP and CE optimization. A specific set of MRM transitions were monitored for each period according to the metabolites eluted within this period. Metabolites with fold change ≥2 or fold change ≤0.05 were assigned as DCMs.
8
Quantitative Metabolite Analysis by Q TRAP MS
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
LIT and triple quadruple (QQQ) scans were acquired on a triple quadruple-linear ion trap mass spectrometer (Q TRAP), API 4500 Q TRAP UPLC/MS/MS System, equipped with an ESI Turbo Ion-Spray interface, operating in positive and negative ion mode and controlled by Analyst 1.6.3 software (AB Sciex). The ESI source operation parameters were as follows: ion source, turbo spray; source temperature 550℃; ion spray voltage (IS) 5500 V (positive ion mode)/-4500 V (negative ion mode); ion source gas I (GSI), gas II (GSII), curtain gas (CUR) were set at 50, 60, and 30.0 psi, respectively; the collision gas (CAD) was high. Instrument tuning and mass calibration were performed with 10 and 100 μmol/L polypropylene glycol solutions in QQQ and LIT modes, respectively. QQQ scans were acquired as MRM experiments with collision gas (nitrogen) set to 5 psi. DP and CE for individual MRM transitions were done with further DP and CE optimization. A specific set of MRM transitions were monitored for each period according to the metabolites eluted within this period.
9
Quantitative Mass Spectrometry Metabolite Profiling
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
LIT and triple quadrupole (QQQ) scans were acquired on a triple quadrupole-linear ion trap mass spectrometer (Q TRAP), API 4500 Q TRAP UPLC/MS/MS System, equipped with an ESI Turbo Ion-Spray interface, operating in positive and negative ion mode and controlled by Analyst 1.6.3 software (AB Sciex). The ESI source operation parameters were as follows: ion source, turbo spray; source temperature 550 °C; ion spray voltage (IS) 5500 V (positive ion mode)/−4500 V (negative ion mode); ion source gas I (GSI), gas II (GSII), and curtain gas (CUR) were set at 50, 60, and 30.0 psi, respectively; the collision gas (CAD) was high. Instrument tuning and mass calibration were performed with 10 and 100 μmol/L polypropylene glycol solutions in the QQQ and LIT modes, respectively. QQQ scans were acquired as MRM experiments with the collision gas (nitrogen) set to 5 psi. DP and CE for individual MRM transitions were performed with further DP and CE optimization. A specific set of MRM transitions was monitored for each period according to the metabolites eluted within this period.
10
Metabolic Profiling of Frozen Samples
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The frozen sample was crushed using a mixer mill (MM 400, Retsch, Germany) with a zirconia bead for 1.5 min at 30 Hz. The resulting powder was weighed and 100 mg was extracted overnight with 1.2 ml 70% aqueous methanol at 4°C. Following centrifugation at 12,000 rpm for 10 min, the extracts were filtered (SCAA-104, 0.22 μm pore size; ANPEL, Shanghai, China) before UPLC-MS/MS analysis. The sample extracts were analyzed using an UPLC-ESI-MS/MS system (UPLC, Shim-pack UFLC SHIMADZU CBM30A system, www.shimadzu.com.cn/ ; MS, Applied Biosystems 4500 Q TRAP, www.appliedbiosystems.com.cn/ ). LIT and triple quadrupole (QQQ) scans were acquired on a triple quadrupole-linear ion trap mass spectrometer (Q-TRAP), API 4500 Q-TRAP UPLC/MS/MS System, equipped with an ESI Turbo Ion-Spray interface, operating in positive and negative ion mode and controlled by Analyst 1.6.3 software (AB Sciex). The criteria for determining differential metabolites were: Fold change ≥2 or ≤0.5, and Variable Importance in Projection (VIP) value ≥1.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!