Bs 1206r

Manufactured by Bioss Antibodies

Sourced in China

The Bs-1206R is a benchtop centrifuge capable of up to 6,000 rpm and 4,000 x g. It features a compact design and can accommodate rotors for tubes ranging from 1.5 mL to 50 mL.

Automatically generated - may contain errors

Lab products found in correlation

System geldoc xr imagelab, by Bio-Rad (1 mentions) Ecl kit, by Boster Bio (1 mentions) Phospho akt ser473, by Cell Signaling Technology (1 mentions) Bca protein concentration assay kit, by Boster Bio (1 mentions) Ar0102 100, by Boster Bio (1 mentions) Ar1183, by Boster Bio (1 mentions) Bs 1544r, by Bioss Antibodies (1 mentions) Ab6672, by Abcam (1 mentions) Ab78486, by Abcam (1 mentions) Ab51983, by Abcam (1 mentions) Bs 1313r, by Bioss Antibodies (1 mentions) Ab1793, by Abcam (1 mentions) Anti ki67 rabbit monoclonal antibody, by Cell Signaling Technology (1 mentions) Cellsens standard imaging software, by Olympus (1 mentions) Ab53281, by Abcam (1 mentions) Ab182136, by Abcam (1 mentions) Ab53715, by Abcam (1 mentions) Anti gal 1 antibody, by Cell Signaling Technology (1 mentions) Goat anti rabbit igg sc 2357, by Santa Cruz Biotechnology (1 mentions) Anti cd34 ab81289, by Abcam (1 mentions)

4 protocols using bs 1206r

Spinal Cord Protein Expression Analysis

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Fresh lumbar spinal cord tissue was extracted and put into RIPA lysate (AR0102-100, Boster), adding protease inhibitor PMSF(AR1178, Boster) and phosphatase inhibitor (AR1183, Boster). BCA protein concentration assay kit (AR0146, Boster) was used to determine the protein concentration.
The total proteins from the spinal cord were separated by electrophoresis using 10% SDS–polyacrylamide gel, then transferred onto PVDF membranes. The membranes were blocked with 5% non-fat milk for 2 h at room temperature, then incubated overnight at 4 °C with the following antibodies: Rabbit Anti-SHH Polyclonal Antibody (bs-1544R, Bioss), Rabbit Anti-Gli-1 Polyclonal Antibody (bs-1206R, Bioss), Phospho-AKT (Ser473, Cell Signaling Technology), AKT polyclonal antibody (AP0095, Bioworld) or β-actin (AP0060, Bioworld) antibodies. After washing, the membranes were incubated with Goat anti-Rabbit IgG (BA1054, Boster) for 1 h at room temperature. The blots were visualized by the chemiluminescence-based detection kit (ECL Kit, Boster). Densitometry analysis was performed with a System Gel Doc XR + IMAGE LAB (Bio-Rad, USA) and quantified with NIH Image software (Image J).

Qi Y., Yang C., Zhao H., Deng Z., Xu J., Liang W., Sun Z, & Nieland J.D. (2022). Neuroprotective Effect of Sonic Hedgehog Mediated PI3K/AKT Pathway in Amyotrophic Lateral Sclerosis Model Mice. Molecular Neurobiology, 59(11), 6971-6982.

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Comprehensive Molecular Profiling of Parotid Tissue

Cited 1 time

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Total RNA extraction, reverse transcription, and quantitative PCR (qPCR) were performed as reported [44 (link),45 (link)]. Primers for swine GAPDH, C1qA, TNF-α, IFN-γ, IL-4, IL-6, P53, AIF1, ADGRE 1, ITGAX, HGF, and ARG 1 were designed using Primer3 software (http://bioinfo.ut.ee/primer3/) and listed in Supplementary Table S1. For protein extraction, fresh parotid samples were homogenized in T-PER reagent containing protease inhibitors (Pierce, WA, USA). The concentration of protein was quantified using BCA, and the loading quantity was 25 μg per lane. Western blotting was done using primary antibodies against AQP5 (1:5000, ab78486), Ptch1 (1:5000, ab51983), TNF-α (1:5000, ab1793), IL-6 (1:200, ab6672), P53 (1:500, ab154036) (these 5 antibodies are from Abcam, Boston, MA, USA), Gli1 (1:1000, bs-1206R), F4/80 (1:1000, bs-7058R), AIF1 (1:1000, bs-1363R), and VEGF (1:100, bs-1313R) (these 4 antibodies are from Bioss, Woburn, MA, USA). All PCR and Western blot analyses were repeated at least three times.

Hu L., Du C., Yang Z., Yang Y., Zhu Z., Shan Z., Zhang C., Wang S, & Liu F. (2021). Transient Activation of Hedgehog Signaling Inhibits Cellular Senescence and Inflammation in Radiated Swine Salivary Glands through Preserving Resident Macrophages. International Journal of Molecular Sciences, 22(24), 13493.

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Immunohistochemical Analysis of Prostate Cancer

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Immunohistochemical staining was performed on TMA and formalin‐fixed, paraffin‐embedded tissues from PC‐3 cell line xenografts. Before staining, sections were dried for 1 hour at 60℃ and deparaffinized in xylene for 30 minutes, after which they were rehydrated by serial incubations in alcohol. Heat‐induced antigen retrieval was performed in citrate buffer at pH 6.0. Sections were subsequently blocked with goat serum and incubated with primary antibody [anti‐β₂‐AR rabbit monoclonal antibody (ab182136; Abcam), anti‐Ki‐67 rabbit monoclonal antibody (9027; Cell Signaling), anti‐Shh rabbit monoclonal antibody (ab53281; Abcam), anti‐Gli1 rabbit polyclonal antibody (bs‐1206R; Bioss, China) or anti‐Ptch1 rabbit polyclonal antibody (ab53715; Abcam) overnight at 4°C (details were shown in Table 1), followed by Histostain‐SP (Streptavidin‐Peroxidase) kit reagent (SP0023; Beijing Biosynthesis Biotec, China), according to the manufacturer's instructions. The diaminobenzidine chromogen (ZSGB Biotec, China) was used for coloration. Slides were finally counterstained with hematoxylin. Negative controls were obtained after the omission of the primary antibody. Stained sections were examined and photographed on a bright‐field microscope (BX53; Olympus) connected to cellSens standard imaging software (Olympus).

Zhang M., Wang Q., Sun X., Yin Q., Chen J., Xu L, & Xu C. (2020). β2‐adrenergic receptor signaling drives prostate cancer progression by targeting the Sonic hedgehog‐Gli1 signaling activation. The Prostate, 80(15), 1328-1340.

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Molecular Mechanisms of GANT61-Mediated Inhibition

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GANT61 was purchased from Sigma Biotechnology (St. Louis, MO, USA). The anti- GAL-1 antibody was purchased from Cell Signaling Technology (13888S and 12936S, Danvers, MA, USA), the anti-GLI1 was purchased from Abcam (ab217326, Cambridge, UK) and Bioss (bs-1206R, Beijing, China), anti-MMP14 (ab78738), anti-MMP2(ab92536), anti-LAMC2 (ab210959 and ab274376) and anti-CD34(ab81289) antibodies were purchased from Abcam (Cambridge, UK), and the anti-GAPDH antibody(sc-47724), horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG(sc-516102) and goat anti-rabbit IgG(sc-2357) were provided by Santa Cruz Biotechnology (Santa Cruz, CA, USA). The PAS staining kit was provided by Leagene Biotechnology Co, Ltd (Beijing, People’s Republic of China). The MTT assay kit and dimethyl sulfoxide (DMSO) were provided by Sigma Biotechnology (St. Louis, MO, USA).

You X., Wu J., Wang Y., Liu Q., Cheng Z., Zhao X., Liu G., Huang C., Dai J., Zhou Y., Chen D, & Chong Y. (2020). Galectin-1 promotes vasculogenic mimicry in gastric adenocarcinoma via the Hedgehog/GLI signaling pathway. Aging (Albany NY), 12(21), 21837-21853.

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