Quantigene plex 2.0 reagentsystem

Manufactured by Panomics

Sourced in United States

The QuantiGene Plex 2.0 Reagent System is a laboratory equipment used for gene expression analysis. It is designed to quantify multiple target RNAs simultaneously from a single sample.

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Lab products found in correlation

Penicillin, by Thermo Fisher Scientific (1 mentions) Streptomycin, by Thermo Fisher Scientific (1 mentions) Emodin, by Merck Group (1 mentions) Fluo 4 calcium assaykit, by Thermo Fisher Scientific (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Phosphate buffered saline (pbs), by Thermo Fisher Scientific (1 mentions) Indomethacin, by Merck Group (1 mentions) Griess reagent, by Merck Group (1 mentions) Poly 1 c, by Merck Group (1 mentions) Lysis buffer, by Bio-Rad (1 mentions)

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QuantiGene Plex 2.0

3 protocols using quantigene plex 2.0 reagentsystem

Multiplex Cytokine and RNA Analysis

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DMEM, FBS, penicillin, streptomycin, PBS, and other tissue culture reagents were
purchased from Gibco BRL (Grand Island, NY, USA). Emodin, poly I:C, indomethacin, Griess
reagent, and all other chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA).
The multiplex bead-based cytokine assay kits used for the determination of cytokine
concentration were purchased from Millipore (Billerica, MA, USA). The Fluo-4 calcium assay
kit was purchased from Molecular Probes (Eugene, OR, USA). QuantiGene Plex 2.0 Reagent
System for direct quantification of multiple RNA targets was purchased from Panomics
(Redwood City, CA, USA).

Kim Y.J., Lee J.Y., Kim H.J., Kim D.H., Lee T.H., Kang M.S., Choi Y.K., Lee H.L., Kim J., An H.J, & Park W. (2017). INHIBITORY EFFECT OF EMODIN ON RAW 264.7 ACTIVATED WITH DOUBLE STRANDED RNA ANALOGUE POLY I:C. African Journal of Traditional, Complementary, and Alternative Medicines, 14(3), 157-166.

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Quantifying STAT1 mRNA in RAW 264.7 cells

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At the end of 24 h incubation with PIC and/or oroxylin A, RAW 264.7 mouse macrophages were lysed using lysis buffer (Bio-Rad Laboratories, Inc.). To simultaneously quantify multiple RNA targets directly from cell lysate, a QuantiGene Plex 2.0 Reagent System (Panomics, Inc., Redwood City, CA, USA) based on branched DNA signal amplification technology with xMAP beads was used according to manufacturer's instructions (15 (link)) and mRNA expression of STAT1 (GenBank no. NM_009283) was determined. Data were normalized against the control, GAPDH (GenBank no. NM_001001303).

LEE J.Y, & PARK W. (2016). Anti-inflammatory effects of oroxylin A on RAW 264.7 mouse macrophages induced with polyinosinic-polycytidylic acid. Experimental and Therapeutic Medicine, 12(1), 151-156.

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Screening Mammalian Secreted Peptides for BAT Activation

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A protein library containing more than 5000 mammalian secreted peptides^{21 (link)} was used. Murine BAT preadipocytes were partially differentiated using 5 µM dexamethasone, 0.25 mM isobutylmethylxanthine (IBMX), and 0.125 mM indomethacin for 48 h. The cells were then treated with test media containing proteins in Dulbecco’s modified Earle’s medium (DMEM) with 10% fetal bovine serum (FBS) for a further 24 h. Ucp1 mRNA induction was assayed following the 24-h treatment. Ucp1 mRNA induction was assayed using a QuantiGene® Plex 2.0 reagent system (Panomics, Fremont, CA).

Shamsi F., Xue R., Huang T.L., Lundh M., Liu Y., Leiria L.O., Lynes M.D., Kempf E., Wang C.H., Sugimoto S., Nigro P., Landgraf K., Schulz T., Li Y., Emanuelli B., Kothakota S., Williams L.T., Jessen N., Pedersen S.B., Böttcher Y., Blüher M., Körner A., Goodyear L.J., Mohammadi M., Kahn C.R, & Tseng Y.H. (2020). FGF6 and FGF9 regulate UCP1 expression independent of brown adipogenesis. Nature Communications, 11, 1421.

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