Camkiiα cre b6 cg tg camk2a cre t29 1stl j

All procedures were approved by the SingHealth Institutional Animal Care and Use Committee. Mecp2-floxed (B6.129P2-Mecp2^tm1Bird/J) and CamKIIα-cre (B6.Cg-Tg(Camk2a-cre)T29-1Stl/J) mice were purchased from Jackson Laboratories. The males were removed from the breeding cages near the end of the gestation period. The subsequent individually-housed dams were kept with their delivered pups until weaning at postnatal day 21 (P21). Weaned pups were housed in cages with up to five same-sex littermates, with food and water provided ad libitum. All animals were maintained on a 12-h light/dark cycle, with lights on at 0700 h and lights off at 1900 h.
Lactating dams were provided with drinking water supplemented with 25 mM choline and 50 mM saccharin, or with 50 mM saccharin only (vehicle). There were two experimental supplementation periods: from the day of birth of the litter (P1) until weaning (P21) (early supplementation), and from 6 weeks to 9 weeks of age (late supplementation).

C57BL/6J and Ca2+/calmodulin‐dependent protein kinase II alpha (CaMKIIα)‐Cre (B6.Cg‐Tg(Camk2a‐cre)T29‐1Stl/J) were obtained from the Jackson Laboratory. Aldh2‐Flox (C57BL/6‐Aldh2em1(flox)Smoc) line was obtained from the Shanghai Model Organisms. Mouse colonies were maintained and bred in the Animal and Plant Care Facility of HKUST and the Laboratory Animal Services Centre of CUHK. All animal experimental protocols were approved both by the Animal Ethics Committees at both HKUST and CUHK, and their care was in accord with both the institutional and Hong Kong guidelines.

The floxed-Cyfip2 and Cyfip2^+/− mice used in this study have been previously described (Han et al., 2015 (link); Zhang et al., 2018 (link), 2019a (link); Lee S. H. et al., 2020 (link)). The CaMKIIα-Cre [B6.Cg-Tg(Camk2a-cre)T29-1Stl/J] and Thy1-YFP [B6.Cg-Tg(Thy1-YFP)HJrs/J] mice were obtained from the Jackson Laboratory. The mice were bred and maintained on a C57BL/6J background according to the Korea University College of Medicine Research Requirements. All procedures were approved by the Committee on Animal Research at Korea University College of Medicine (KOREA-2018-0174). The mice were fed ad libitum and housed under a 12 h light-dark cycle. All experiments were performed with an adult (8 to 10-week old) male Cyfip2 mice and their littermate controls.