Dapi stain

Cdc42 and RhoA biosensors were kind gifts from Dr. Louis Hodgson (Albert Einstein College of Medicine). RhoA/Rac1/Cdc42 Activation Assay Combo Kit was purchased from Cell BioLabs (Sand Diego, CA, USA). QCM Gelatin Invadopodia Assay was obtained from Millipore (Massachusetts, USA). Primary antibodies against Cdc42, StarD13, actin, vinculin, Arp2, cortactin (Mouse and Rabbit) and TKS5 antibodies were obtained from Abcam (Cambridge, UK). Primary mouse anti-TKS4 was obtained from Merck Millipore. Primary mouse anti-StarD13 and rabbit anti-Cdc42 were obtained from Santa Cruz Biotech. HRP-conjugated secondary antibodies were obtained from Promega (Wisconsin, USA). Fluorescent secondary antibodies Alexa Fluor 488-green and Alexa Fluor 594-red as well as Rhodamine-phalloidin stain were purchased from Invitrogen (Massachusetts, USA). DAPI stain, and cell proliferation reagent were acquired from Roche Diagnostics (Roche Ltd, Mannheim, Germany). Hiperfect transfection reagent, luciferase and human Flexi Tubes siRNA for luciferase, StarD13, Cdc42 and RhoA were obtained from Qiagen (Hilden, Germany). Lipofectamine LTX was from Waltham (Massachusetts, USA) and crystal violet was from SCP Science (Quebec, Canada).

Ethylene diamine tetraacetic acid (EDTA), ascorbic acid, sodium glycerophosphate, dexamethasone, cetylpyridinium bromide, alizarin red S and Triton-X100 were obtained from Sigma-Aldrich (USA). Foetal bovine serum (FBS), αMEM medium, non-essential amino acid (NEAA), β-mercaptoethanol, L-glutamax and penicillin/streptavidin were purchased from Gibco (USA). Cell culture plates and Matrigel were bought from Corning (USA). Methanol, absolute ethanol, chloroform, hydrochloric acid and isopropanol were obtained from BCIGC (China). Bovine serum albumin (BSA), phosphate buffer, N-hydroxysulfosuccinimide sodium salt (NHSS) and 1-ethyl-3-(3-dimethylamino propyl) carbodiimide (EDC) were purchased from Aladdin (China). BCIP/NBT alkaline phosphatase colouring kit and ALP quantitative detection kit was acquired from CWBIO (China). SYBR Green I and TRIzol were bought from Takara (Japan). Paraformaldehyde was obtained from Solarbio (China). Quartz crystal microbalance chips were obtained from HRbio (China). Cell counting kit-8 (CCK8) was purchased from Dojindo (Japan). RevertAid™ First Stand cDNA Synthesis Kit was gained from Thermo (USA). Cell cycle assay reagent was obtained from KoradBio (China). E8 medium was acquired from Cellapy (China). DAPI stain was purchased from Roche (Switzerland). Carboxyl functionalized QCM chips were provided by Dongwei BiologicalTechnology Co., LTD (China).