C myc antibody sc 40

GluR1 antibody (SC-55509), GluR2 antibody (SC-517265), STAT3 antibody (SC-482), pY705-STAT3 antibody (SC-7993), c-Myc antibody (SC-40), and HA-probe antibody (SC-7392) were from Santa Cruz Biotechnology (Santa Cruz). Acetylated-lysine antibody (9441), acK685-STAT3 antibody (2523), pS727-STAT3 antibody (9136), P70/85 S6 kinase antibody (9202), pERK1/2 antibody (9106), and ERK1/2 antibody (9102) were from Cell Signaling Technology (Boston). H3 antibody (ab1791) and VDAC1/porin antibody (ab14734) were from Abcam. Flag antibody (F9291) and tubulin antibody (T2200) were from Sigma.

PML antibodies were purchased from Santa Cruz (sc-966) for immunofluorescent staining, or from Bethyl (A301–167A) for immunoblotting. c-Myc antibody (sc-40) and Sox2 antibody (sc-17320) were purchased from Santa Cruz. PARP antibody (9542) and cleaved Caspase-3 antibody (9661S) were from Cell Signaling. Glut1 antibody was from Thermo Scientific (PA1–37782). Arsenic trioxide was purchased from Sigma (202673–5G) and the stock solution was prepared at 100 mM as instructed by manufacturer.

Two-week-old seedlings under control conditions or after various treatments were analyzed for gene expression using quantitative real time PCR analysis. Total RNA was extracted using a TRIzol Kit (TaKaRa), and a PrimeScript^TM RT reagent Kit (Perfect Real Time, TaKaRa) was used for reverse transcription. Real-time PCR was performed in an ABI 7500 system with the SYBR^® Premix DimerEraser^TM (Perfect Real Time, TaKaRa), each reaction consisted of 20 ng of cDNA, 0.1 μM primers and 10 μL 2x SYBR Premix ExTaq in a final volume of 20 μL. The reactive cycle used 95°C for 30 s, then 40 cycles at 95°C for 5 s and 60°C for 34 s. The gene expression quantity was calculated using the relative 2^-ΔΔCT method as previously reported (Li et al., 2013 (link)). The relative expression level was normalized to that of ACT2 internal control. Primers used for gene expression analysis are listed in Supplementary Table S1. Assessment of NAC072 and ABF3 protein levels was carried out using western blot (WB) with Flag (F3165, Sigma) or c-Myc antibody (sc-40, Santa Cruz) as previously described (Sakuraba et al., 2015 (link)). The β-glucuronidase (GUS) activity of the RD29A and RD29B promoters was previously described (Woo et al., 2004 (link)). The presented data are the average of three technical replicates, and experiments were biologically repeated at least three times with similar results.